Person: Gutiérrez Cepeda, Luna
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First Name
Luna
Last Name
Gutiérrez Cepeda
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Medicina y Cirugía Animal
Area
Medicina y Cirugía Animal
Identifiers
4 results
Search Results
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Item Comparative Kinematic Analysis of Hurdle Clearance Technique in Dogs: A Preliminary Report(Animals, 2020) Miró, F.; López, P.; Vilar, J.M.; Galisteo, A.M.; Vivo, J.; Garrido-Castro, J.L.; Gutiérrez Cepeda, LunaAlthough the jumping characteristics of agility dogs have been examined in recent years, there is currently a lack of data related to the suspension phase. The purpose of the present study was to investigate the biomechanics of the suspension phase of the agility jump and to analyze the kinematic differences in dogs with different jumping abilities. Two groups of dogs of the same height category (large dogs) competing at different skill levels and assessed as excellent jumpers (n = 4) and less-skilled jumpers (n = 3), respectively, were analyzed and statistically compared. Excellent jumpers showed longer and faster jumps with flatter jump trajectories than less-skilled jumpers. In less-skilled jumpers, the distance in front of the hurdle was notably greater than the distance behind it, while the difference between these two distances was less in excellent jumpers. Length and duration of the jump, maximal height of the jumping trajectory, take-off and landing distances to the hurdle, time of occurrence of maximal jump height, and time of change in back orientation essentially defines the suspension phase of the agility jump. This study presents preliminary evidence that the kinematic characteristics of hurdle clearance are different in excellent jumper dogs and in less-skilled jumper dogs.Item Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation(Animals (based), 2022) Bláquez Sarro, Juan Carlos; Gutiérrez Cepeda, Luna; Crespo Castejón, Francisco; Serres Dalmau, María ConsolacionThe Purebred Spanish Horse, according to our clinical experience, is characterized by having a high number of stallions that do not meet the international commercial recommendations forequine-sperm cryopreservation. This means that artificial insemination with frozen semen from these stallions is less widespread than in other breeds. In this study, we investigated if the incorporation ofsingle-layer colloidal centrifugation prior to cryopreservation in clinical conditions could increase the number of ejaculates of Purebred Spanish stallions suitable for this processing, observing the influence of centrifugation and freezing extender protocol on post-thawed sperm motility. Using colloidal centrifugation, the percentage of ejaculates available to be frozen was increased from 35% (6/17) to71% (12/17), doubling the number of samples that could have been subjected to cryopreservation. We only found significant differences in linearity (LIN) and lateral head displacement (ALH) after5 min of incubation at 37 ◦C between colloidal and simple centrifugation processing techniques. No significant differences were found between the two different colloidal protocols in any of the variables considered. Colloidal centrifugation allowed us to obtain, from worse fresh-quality ejaculates, thawed sperm doses with similar quality to that of good-quality ejaculates. BotuCrio® produced, in general higher motility parameters and its characteristics than the other extenders analyzed, with significant differences found in comparison to Inra-Freeze® and Lac-Edta in both total (MOT) and progressive motility (PMOT) when using colloidal centrifugation and only in PMOT when applying simple centrifugation. Colloidal centrifugation optimized the efficiency of cryopreservation, as it allowed usto increase the number of ejaculates of Purebred Spanish Horses suitable to be frozen. Including these semen processing techniques in the freeze test could help to optimize equine-sperm cryopreservation protocols, especially when dealing with individuals or breeds for which initially low sperm quality prevents or limits their inclusion in sperm cryopreservation programsItem Electromyographic and ultrasonographic evaluation of neuromuscular electrical stimulation training on the equine rectus abdominis muscle(Comparative Exercise Physiology, 2019) Hernández-Fernández, Tatiana; Gutiérrez Cepeda, Luna; López-Sanromán, Javier; Manso Díaz, Gabriel; Cediel Algovia, RafaelThe current study aimed to determine the effects of neuromuscular electrical stimulation (NMES) on equine rectus abdominis using surface electromyography (sEMG) and ultrasonographic muscle thickness evaluation. Five horses were trained with NMES for 12 weeks; muscle thickness and sEMG evaluations were obtained before and after the training period. Three different tests were carried out for sEMG evaluations: Test A tried to elicit a voluntary maximal isometric contraction (VMIC); Test B used NMES to elicit a muscular contraction; and Test C used 1 ms repetitive electrical impulses to elicit a series of M-waves. Muscle strength was evaluated from the root mean square (RMS; Tests A and B) and peak to peak (PP; Test C) values obtained from the sEMG data. Measures related to amplitude (RMSa; RMSm; RMSa; PP) were normalised with their pre-training values for every muscle prior to statistical analysis, leading to values as a proportion of the initial value. The evaluation of muscle fatigue was performed using a Fourier test analysis of the frequency range, obtaining the median frequency (MF) for all tests. Muscle thickness (MT) was measured by ultrasound of left and right sides of the rectus abdominis. Data were analysed using non parametric test of Wilcoxon (Test A RMSa; Test A RMSm; Test B RMSa; Test B RMSm; Test C PP; MT; P<0.05) and t-test (Test A MF; Test B MF; Test C MF; P<0.05). Statistical differences were observed between baseline and trained horses. Muscle force increase following NMES training in Test B (RMSa 2.50±0.69; RMSm 2.59±0.76) and Test C (PP 5.20±1.76). Fatigue of the rectus abdominis decreased in Test A (168.33±55.19 vs 232.63±44.15 Hz) and Test C MF (187.93±20.76 vs 236.98±52.39 Hz), but not in Test B (363.98±45.48 vs 327.95±50.84 Hz). The difference in muscle thickness between the two groups was not significant (10.96±0.64; 11.78±0.79 mm). The results suggest that NMES training could be used as an effective method to increase muscle force and fatigue resistance of the rectus abdominis muscle in the horse.Item The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA(Acta Veterinaria Scandinávica, 2012) Gutiérrez Cepeda, Luna; Fernández, Alvaro; Crespo Castejón, Francisco; Ramírez, Miguel Ángel; Gosálvez Berenguer, Jaime; Serres Dalmau, María ConsolacionVariability among stallions in terms of semen cryopreservation quality renders it difficult to arrive at a standardized cryopreservation method. Different extenders and processing techniques (such us colloidal centrifugation) are used in order to optimize post-thaw sperm quality. Sperm chromatin integrity analysis is an effective tool for assessing such quality. The aim of the present study was to compare the effect of two single layer colloidal centrifugation protocols (prior to cryopreservation) in combination with three commercial freezing extenders on the post-thaw chromatin integrity of equine sperm samples at different post-thaw incubation (37°C) times (i.e., their DNA fragmentation dynamics). Results Post-thaw DNA fragmentation levels in semen samples subjected to either of the colloidal centrifugation protocols were significantly lower (p<0.05) immediately after thawing and after 4 h of incubation at 37°C compared to samples that underwent standard (control) centrifugation. The use of InraFreeze® extender was associated with significantly less DNA fragmentation than the use of Botu-Crio® extender at 6 h of incubation, and than the use of either Botu-Crio® or Gent® extender at 24 h of incubation (p<0.05). Conclusions These results suggest that single layer colloidal centrifugation performed with extended or raw semen prior to cryopreservation reduces DNA fragmentation during the first four hours after thawing. Further studies are needed to determine the influence of freezing extenders on equine sperm DNA fragmentation dynamics.