Person:
Cuesta Martínez, Ángel

Profile Picture
First Name
Ángel
Last Name
Cuesta Martínez
URI
https://hdl.handle.net/20.500.14352/77488
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Bioquímica y Biología Molecular
Area
Bioquímica y Biología Molecular
Identifiers
UCM identifierORCIDScopus Author IDWeb of Science ResearcherIDDialnet ID

Filters

20111
Reset filters

Settings

Author: Sánchez-Martín, David × Subject: 577.2 ×

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    The Multicompartmental p32/gClqR as a New Target for Antibody-based Tumor Targeting Strategies
    (Journal of Biological Chemistry, 2011) Sánchez-Martín, David; Fogal, Valentina; Ruoslahti, Erkki; Álvarez-Vallina, Luis; Cuesta Martínez, Ángel
    Tumor-associated cell surface antigens and tumor-associated vascular markers have been used as a target for cancer intervention strategies. However, both types of targets have limitations due to accessibility, low and/or heterogeneous expression, and presence of tumor-associated serum antigen. It has been previously reported that a mitochondrial/cell surface protein, p32/gC1qR, is the receptor for a tumor-homing peptide, LyP-1, which specifically recognizes an epitope in tumor cells, tumor lymphatics, and tumor-associated macrophages/myeloid cells. Using antibody phage technology, we have generated an anti-p32 human monoclonal antibody (2.15). The 2.15 antibody, expressed in single-chain fragment variable and in trimerbody format, was then characterized in vivo using mice grafted subcutaneously with MDA-MB-231 human breast cancers cells, revealing a highly selective tumor uptake. The intratumoral distribution of the antibody was consistent with the expression pattern of p32 in the surface of some clusters of cells. These results demonstrate the potential of p32 for antibody-based tumor targeting strategies and the utility of the 2.15 antibody as targeting moiety for the selective delivery of imaging and therapeutic agents to tumors.