Person:
Carracedo Rodríguez, Juan Gonzalo

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First Name
Juan Gonzalo
Last Name
Carracedo Rodríguez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
Identifiers
UCM identifierORCIDScopus Author IDDialnet IDGoogle Scholar ID

Search Results

Now showing 1 - 10 of 24
  • Publication
    Dual-Mode Gold Nanoparticle-Based Method for Early Detection of Acanthamoeba
    (MDPI, 2022-11-28) Pastrana, Cristina; Guerreiro, Joana Rafaela L.; Elumalai, Monisha; Carpena Torres, Carlos; Crooke, Almudena; Carracedo Rodríguez, Juan Gonzalo; Prado, Marta; Huete Toral, Fernando
    Acanthamoeba keratitis is an aggressive and rapidly progressing ocular pathology whose main risk factor is the use of contact lenses. An early and differential diagnosis is considered the main factor to prevent the progression and improve the prognosis of the pathology. However, current diagnosis techniques require time, complex and costly materials making an early diagnosis challenging. Thus, there is a need for fast, accessible, and accurate methods for Acanthamoeba detection by practitioners for timely and suitable treatment and even for contact lens user as preventive diagnosis. Here, we developed a dual-mode colorimetric-based method for fast, visual, and accurate detection of Acanthamoeba using gold nanoparticles (AuNPs). For this strategy, AuNPs were functionalized with thiolated probes and the presence of target Acanthamoeba genomic sequences, produce a colorimetric change from red to purple. This approach allows the detection of 0.02 and 0.009 μM of the unamplified Acanthamoeba genome by the naked eye in less than 20 min and by color analysis using a smartphone. Additionally, real samples were successfully analyzed showing the potential of the technology considering the lack of point-of-care tools that are mostly needed.
  • Publication
    PhDAY 2020 -FOO (Facultad de Óptica y Optometría)
    (Facultad de Óptica y Optometría (UCM), 2020) Carpena Torres, Carlos; Pintor, Jesús; Pérez de Lara, María Jesús; Toral, Fernando; Crooke, Almudena; Pastrana, Cristina; Carracedo Rodríguez, Juan Gonzalo; Cayuela López, Ana; Sorzano Sánchez, Óscar; Charbel, Carla; Garzón Jiménez, Nuria; Carballo Álvarez, Jesús; Diz Arias, Elena; Fernández Jiménez, Elena; Peral Cerdá, Assumpta; Gómez Pedrero, José Antonio; Durán Prieto, Elena; López Alonso, José Manuel; Fernández Torres, Miguel Ángel; Guzmán Aránguez, Ana Isabel; Gómez Manzanares, Ángela; Vázquez Moliní, Daniel; Martínez Antón, Juan Carlos; Bernárdez Vilaboa, Ricardo; Mayorga Pinilla, Santiago; Álvarez Fernández-Balbuena, Antonio; Benítez, AntoJ.; El Youssfi, Asmae Igalla; León Álvarez, Alejandro; Palomo Álvarez, Catalina; LLedó Mayans, Victoria Eugenia; Awad Alkozi, Hanan; Sánchez Naves, Juan; Martínez Alberquilla, Irene; García Montero, María; Ruiz Alcocer, Javier; Madrid Costa, David; Martínez Florentín, Gema; Papas, Eric B.; Medrano Muñoz, Sandra Milena; Molina, Nancy; Jurado, Sandra; Oliveiros López, Juan; Platero Alvarado, Nadiuska Cristine; Garrido Mercado, Rafaela; Pérez Garmendia, Carlos; Antona Peñalba, Beatriz; Barrio de Santos, Ana Rosa; González Pérez, Mariano; Pérez Garmendia, Carlos; Serramito Blanco, María; Privado Aroco, Ana; Almalki, Wael; Bodas Romero, Julia; Ouzzani, Mohamed; Paune, Jaume; Calderón García, Raquel; Pitarch Velasco, Aida; Cebrián, José Luis; Sánchez Pérez, Isabel; García Rojo, Marta María; Bonnin Arias, Cristina; Sánchez Ramos, Celia; Gutiérrez Jorrín, Sara Carmen; Rodríguez Alonso, Xabier; Laucirica Sáenz, Gorka; Arranz Márquez, Esther; Alonso Castellanos, Miriam; Teus Guezala, Miguel Ángel; Hernández Verdejo, José Luis; Mármol Errasti, Esther; Martín García, Beatriz; Arriola Villalobos, Pedro; Gómez de Liaño, Rosario; Mínguez Caro, N; Orduña Azcona, Javier; Navarro Gil, Francisco Javier; Huete Toral, Fernando; Rodríguez Pomar, Candela; Pastrana Robles, Cristina; Martínez Águila, Alejandro; Martín Gil, Alba; Tomé de la Torre, Miguel Ángel
    Por cuarto año consecutivo los doctorandos de la Facultad de Óptica y Optometría de la Universidad Complutense de Madrid cuentan con un congreso propio organizado por y para ellos, el 4º PhDAY- FOO. Se trata de un congreso gratuito abierto en la que estos jóvenes científicos podrán presentar sus investigaciones al resto de sus compañeros predoctorales y a toda la comunidad universitaria que quiera disfrutar de este evento. Apunta en tu agenda: el 15 de octubre de 2020. En esta ocasión será un Congreso On-line para evitar que la incertidumbre asociada a la pandemia Covid-19 pudiera condicionar su celebración.
  • Publication
    Melatonin-Eluting Contact Lenses Effect on Tear Volume: In Vitro and In Vivo Experiments
    (MDPI, 2022-05) Serramito Blanco, María; Pereira da Mota, Ana Filipa; Carpena Torres, Carlos; Huete Toral, Fernando; Álvarez Lorenzo, Carmen Isabel; Carracedo Rodríguez, Juan Gonzalo
    Background: The purpose of this study was to synthesize melatonin-eluting contact lenses (CLs) and evaluate both the ocular kinetics of the released melatonin and its effect on tear volume and intraocular pressure. Methods: In vitro, melatonin-eluting CLs were synthesized by using non-functionalized (HEMA) and functionalized (HEMA/APMA) monomers. In vivo, a short-term prospective and randomized study was performed on 15 rabbits divided into two groups: 12 rabbits wearing functionalized CLs and 3 rabbits without CLs as a control. The melatonin levels in tears, aqueous humor, vitreous body and retina, tear volume, and intraocular pressure were measured for 8 h. Results: In vitro, both monomers did not show differences in terms of melatonin loading and release (p ≥ 0.05). In vivo, the melatonin concentration was elevated in tears and aqueous humor after 2 and 4 h of wearing CLs, respectively (p < 0.05). Additionally, the CLs increased tear volume for 2 h (p < 0.05). Conclusions: The melatonin-eluting CLs released their content over the ocular surface for at least 2 h, which was associated with a secretagogue effect on tear volume. However, the increased amount of melatonin found in the aqueous humor had no effect on intraocular pressure.
  • Publication
    Efficacy of artificial tears based on an extract of artemia salina containing dinucleotides in a rabbit dry eye model
    (MDPI, 2021-11-05) Carpena Torres, Carlos; Pintor, Jesús; Huete Toral, Fernando; Martín Gil, Alba; Rodríguez Pomar, Candela; Martínez Águila, Alejandro; Carracedo Rodríguez, Juan Gonzalo
    Background: Artemia salina is a brine shrimp containing high concentrations of dinu-cleotides, molecules with properties for dry eye treatment. For this reason, the purpose of the study was to evaluate the effect of the artificial tears based on an extract of Artemia salina in a rabbit dry eye model. Methods: A prospective and randomized study was carried out. Twenty rabbits were divided into 4 groups (n = 5, each group): healthy rabbits, dry eye rabbits, dry eye rabbits treated with hypromellose (HPMC), and dry eye rabbits treated with Artemia salina. Dry eye was induced by the topical instillation of 0.2% benzalkonium chloride. The measurements were performed before and after the treatment for 5 consecutive days. Results: The topical instillation of artificial tears containing Artemia salina showed beneficial effects on tear secretion, tear break-up time, corneal staining, the density of Goblet cells, heigh of mucin cloud secreted by these cells, and mRNA levels of IL-1β and MMP9 in conjunctival cells. Compared with the HPMC, there was a statistically significant improvement (p < 0.05) with the Artemia salina in all the variables under study, except for the conjunc-tival hyperemia, density of Goblet cells, and mRNA levels of IL-6. Conclusions: The potential of artificial tears based on Artemia salina as a secretagogue agent for dry eye treatment was confirmed, opening the door for future clinical trials and studies to extrapolate the findings for dry eye patients. © 2021 by the authors. Licensee MDPI, Basel, Switzerland
  • Publication
    Oxidative Stress in the Anterior Ocular Diseases: Diagnostic and Treatment
    (MDPI, 2023-01-20) Dammak, Azza; Pastrana, Cristina; Martín Gil, Alba; Carpena Torres, Carlos; Peral Cerdá, Assumpta; Simovart, Mirjam; Alarma-Estrany, Pilar; Huete Toral, Fernando; Carracedo Rodríguez, Juan Gonzalo
    The eye is a metabolically active structure, constantly exposed to solar radiations making its structure vulnerable to the high burden of reactive oxygen species (ROS), presenting many molecular interactions. The biomolecular cascade modification is caused especially in diseases of the ocular surface, cornea, conjunctiva, uvea, and lens. In fact, the injury in the anterior segment of the eye takes its origin from the perturbation of the pro-oxidant/antioxidant balance and leads to increased oxidative damage, especially when the first line of antioxidant defence weakens with age. Furthermore, oxidative stress is related to mitochondrial dysfunction, DNA damage, lipid peroxidation, protein modification, apoptosis, and inflammation, which are involved in anterior ocular disease progression such as dry eye, keratoconus, uveitis, and cataract. The different pathologies are interconnected through various mechanisms such as inflammation, oxidative stress making the diagnostics more relevant in early stages. The end point of the molecular pathway is the release of different antioxidant biomarkers offering the potential of predictive diagnostics of the pathology. In this review, we have analysed the oxidative stress and inflammatory processes in the front of the eye to provide a better understanding of the pathomechanism, the importance of biomarkers for the diagnosis of eye diseases, and the recent treatment of anterior ocular diseases.
  • Publication
    Influence of Circadian Rhythm in the Eye: Significance of Melatonin in Glaucoma
    (MDPI, 2021-02-24) Martínez Águila, Alejandro; Martín Gil, Alba; Carpena Torres, Carlos; Pastrana, Cristina; Carracedo Rodríguez, Juan Gonzalo
    Circadian rhythm and the molecules involved in it, such as melanopsin and melatonin, play an important role in the eye to regulate the homeostasis and even to treat some ocular conditions. As a result, many ocular pathologies like dry eye, corneal wound healing, cataracts, myopia, retinal diseases, and glaucoma are affected by this cycle. This review will summarize the current scientific literature about the influence of circadian patterns on the eye, focusing on its relationship with increased intraocular pressure (IOP) fluctuations and glaucoma. Regarding treatments, two ways should be studied: the first one, to analyze if some treatments could improve their effect on the ocular disease when their posology is established in function of circadian patterns, and the second one, to evaluate new drugs to treat eye pathologies related to the circadian rhythm, as it has been stated with melatonin or its analogs, that not only could be used as the main treatment but as coadjutant, improving the circadian pattern or its antioxidant and antiangiogenic properties.
  • Publication
    Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride
    (Hindawi Publishing Corporation, 2020-05-30) Carpena Torres, Carlos; Pintor, Jesús; Pérez de Lara, María Jesús; Huete Toral, Fernando; Crooke, Almudena; Pastrana, Cristina; Carracedo Rodríguez, Juan Gonzalo
    Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia (P < 0.001), corneal staining (P < 0.001), conjunctival hyperemia (P < 0.001), and levels of IL-6 mRNA (P = 0.005) compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia (P < 0.001), tear breakup time (P = 0.007), tear osmolarity (P < 0.001), density of goblet cells (P < 0.001), and height of mucin cloud (P < 0.001). Conclusions. )e topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).
  • Publication
    A Comparison between Automated Subjective Refraction and Traditional Subjective Refraction in Keratoconus Patients
    (Lippincott Williams & Wilkins, 2021-06) Carracedo Rodríguez, Juan Gonzalo; Carpena Torres, Carlos; Pastrana, Cristina; Privado Aroco, Ana; Serramito Blanco, María; Espinosa Vidal, Teresa M.; Rodríguez Lafora, María
    SIGNIFICANCE: The performance of the Eye Refract (Luneau Technology, Chartres, France), a new instrument to perform aberrometry-based automated subjective refraction, has been previously evaluated in healthy subjects. However, its clinical implications in other ocular conditions are still unknown. PURPOSE: The purpose of this study was to evaluate the agreement between the Eye Refract and the traditional subjective refraction, as the criterion standard, in keratoconus patients with and without intracorneal ring segments (ICRSs). METHODS: A total of 50 eyes of 50 keratoconus patients were evaluated, dividing the sample into 2 groups: 27 eyes without ICRS (37.78 ± 9.35 years) and 23 eyes with ICRS (39.26 ± 13.62 years). An optometrist conducted the refraction with the Eye Refract, and another different optometrist conducted the traditional subjective refraction on the same day. Spherical equivalent (M), cylindrical vectors (J0 and J45), and corrected distance visual acuity were compared between both methods of refraction. In addition, Bland-Altman analysis was performed to assess the agreement between both methods of refraction. RESULTS: There were no statistically significant differences (P ≥ .05) between the Eye Refract and the traditional subjective refraction for all the variables under study in either group. Without ICRS, the mean difference and 95% limits of agreement (upper, lower) were −0.20 (+1.50, −1.89) D for M, −0.14 (+1.40, −1.68) D for J0, and +0.05 (+1.23, −1.14) D for J45. With ICRS, these values worsened to −0.62 (+3.89, −5.12) D for M, +0.06 (+2.46, −2.34) D for J0, and −0.02 (+2.23, −2.28) D for J45. CONCLUSIONS: The Eye Refract seems to offer similar results compared with the traditional subjective refraction in keratoconus patients not implanted with ICRS. However, some patients could show abnormal measurements, especially those with ICRS, who should be treated with caution in clinical practice.
  • Publication
    Contact Lenses as Drug Delivery System for Glaucoma: A Review
    (MDPI, 2020-07-27) Peral Cerdá, Assumpta; Martínez Águila, Alejandro; Pastrana, Cristina; Huete Toral, Fernando; Carpena Torres, Carlos; Carracedo Rodríguez, Juan Gonzalo
    Glaucoma is an optical neuropathy associated to a progressive degeneration of retinal ganglion cells with visual field loss and is the main cause of irreversible blindness in the world. The treatment has the aim to reduce intraocular pressure. The first therapy option is to instill drugs on the ocular surface. The main limitation of this is the reduced time of the drug staying on the cornea. This means that high doses are required to ensure its therapeutic effect. A drug-loaded contact lens can diffuse into the post lens tear film in a constant and prolonged flow, resulting in an increased retention of the drug on the surface of the cornea for up to 30 min and thus providing a higher drug bioavailability, increasing the therapeutic efficacy, reducing the amount of administered drug, and thereby provoking fewer adverse events. Several different systems of drug delivery have been studied in recent decades; ranging from more simple methods of impregnating the lenses, such as soaking, to more complex ones, such as molecular imprinting have been proposed. Moreover, different drugs, from those already commercially available to new substances such as melatonin have been studied to improve the glaucoma treatment efficacy. This review describes the role of contact lenses as an innovative drug delivery system to treat glaucoma.
  • Publication
    Changes in visual quality with soft contact lenses after the instillation of hyaluronic acid eye drops
    (Elsevier, 2021-05-26) Carpena Torres, Carlos; Pastrana, Cristina; Rodríguez Pomar, Candela; Serramito Blanco, María; Batres Valderas, Laura; Carracedo Rodríguez, Juan Gonzalo
    Purpose: To evaluate the changes in visual function and anterior surface aberrations during soft contact lens (SCL) wear after the instillation of hyaluronic acid (HA) eye drops with different viscosity. Methods: A prospective, randomized, and participant-masked study was performed. Twenty healthy participants (25.4 ± 2.6 years) were evaluated. Hydrogel (Ocufilcon D) and silicone-hydrogel (Somofilcon A) SCL were randomly assigned to both eyes of the same participant. Visual function in terms of high- and low-contrast corrected distance visual acuity (CDVA) and anterior contact lens surface aberrations (RMS HOA) were measured before and after the instillation, at different times, of different eye drops: saline (control) and 0.1%, 0.2%, and 0.3% HA. Results: Compared with the saline solution, during hydrogel SCL wear, there was an improvement (P < 0.05) in high-contrast CDVA after 3 and 10 min with 0.1% HA, and after 5 and 20 min with 0.2% HA. During silicone�hydrogel SCL wear, there was a deterioration (P < 0.05) in high-contrast CDVA after 1 and 30 min with 0.3% HA. Additionally, during silicone-hydrogel SCL wear, there was also a deterioration (P < 0.05) in low-contrast CDVA after 5 and 20 min with 0.3% HA. In terms of RMS HOA, there were no clinically relevant changes with both SCL. Conclusions: The instillation of HA eye drops could have a different effect on visual quality depending on their concentration of HA, the contact lens material, its surface ionicity, or other physicochemical properties that should be studied in future studies.