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Gómez Gómez, Beatriz

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Beatriz
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Gómez Gómez
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https://hdl.handle.net/20.500.14352/82513
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Universidad Complutense de Madrid
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2021 - 20247
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Author: Moreno Martín, Gustavo × Has files: true ×

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    Project number: 202
    ODS con mucha ciencia: Hacia una enseñanza STEM y más allá
    (2022) Gracia Lor, Emma; Blanco Asenjo, Miriam; Espada Bernabé, Elena; Fernández Bautista, Tamara; Gómez Castro, Emilio; Gómez Mejía, Esther; Gómez Gómez, Beatriz; Lorente Arévalo, Álvaro; Moreno Martín, Gustavo; Muñoz Olivas, María Riansares; Muñoz San Martín, Cristina; Oro Carretero, Paloma de; Pérez Corona, María Teresa; Romero Sánchez, Iván; Sacristán Navarro, Iván; Vicente Zurdo, David
    Memoria del proyecto de innovación titulado "ODS con mucha ciencia: Hacia una enseñanza STEM y más allá" de la convocatoria 2021-2022. El proyecto se ha centrado en fomentar el interés de los estudiantes de ESO y Bachillerato por la ciencia, proporcionándoles herramientas para que desarrollen competencias STEM a través de charlas de divulgación, talleres experimentales y sesiones de mentoría.
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    Aprendizaje-Servicio para trasladar los resultados desarrollo sostenible: del laboratorio a la sociedad
    (2021) Gracia Lor, Emma; Gómez Mejía, Esther; Moreno Martín, Gustavo; Gómez Gómez, Beatriz; Vicente Zurdo, David; Pérez Corona, María Teresa; Muñoz Olivas, María Riansares; Gómez Castro, Emilio
    El objetivo general planteado en este proyecto ha sido comunicar y concienciar a los estudiantes de secundaria y bachillerato sobre la necesidad de un desarrollo sostenible, con el fin de que sean capaces de comprender e identificar las problemáticas asociadas a los objetivos planteados en la Agenda 2030 y asociarlas con actividades propias de la vida cotidiana. De esta manera, los alumnos podrán adquirir herramientas de gran utilidad para el desarrollo de su futura vida personal y profesional. Para lograr dichos objetivos se han llevado a cabo una serie de actividades (charlas de divulgación científica, debates, uso de la aplicación Kahoot) en diferentes centros educativos no universitarios, en concreto, en tres institutos de educación secundaria y bachillerato (IES) y en un centro de educación de personas adultas (CEPA). Los temas tratados se encuadran dentro de los Objetivos de Desarrollo Sostenible (ODS) de la Agenda 2030, concretamente los ODS 2, 3 y 12.
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    Project number: 283
    La divulgación científica como herramienta para promover el desarrollo sostenible: de la universidad a la sociedad
    (2021) Gracia Lor, Emma; Muñoz Olivas, María Riansares; Pérez Corona, María Teresa; Gómez Castro, Emilio; Gómez Mejía, Esther; Moreno Martín, Gustavo; Vicente Zurdo, David; Gómez Gómez, Beatriz
    Memoria del proyecto de innovación titulado "La divulgación científica como herramienta para promover el desarrollo sostenible: de la universidad a la sociedad" de la convocatoria 2020-2021. El proyecto se ha centrado en promover una educación de calidad mediante la transferencia de conocimientos científicos relacionados con los Objetivos de Desarrollo Sostenible 2, 3 y 12 de la Agenda 2030 y el desarrollo de pensamiento crítico científico-divulgativo tanto del alumnado procedente de centros educativos de secundaria y bachillerato como de los estudiantes de universidad.
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    Evaluation of the transformation of selenite and selenium nanoparticles to seleno-amino acids produced by Escherichia coli and Staphylococcus aureus by using liquid chromatography -inductively coupled plasma mass spectrometry and single-particle- inductively coupled plasma mass spectrometry and different sample treatments
    (Spectrochimica Acta Part B: Atomic Spectroscopy, 2023) Moreno Martín, Gustavo; Espada-Bernabé, Elena; Gómez Gómez, Beatriz; León González, María Eugenia De; Madrid Albarrán, María Yolanda
    Due to the scarce knowledge about the impact of selenium nanoparticles (SeNPs) on bacterial populations, the main objective of this work was focused on evaluating the transformations of SeNPs and selenite in Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). For this purpose, an analytical methodology based on bacteria cell wall disruption, carbamidomethylation, enzymatic hydrolysis and high-performance liquid chromatography couple to inductively coupled plasma mass spectrometry (HPLC-ICP-MS) and HPLC- electrospray tandem mass spectrometry (HPLC-ESI-MS/MS) measurements was developed, the latter for unambiguous identification of selenium species. Once bacteria were cultured in the presence of chitosan modified SeNPs (Ch-SeNPs) and selenite at 0, 1 and 2 mg L􀀀 1 Se for 24 h, an enzymatic disruption of the bacterial cell wall using lysozym followed by enzymatic hydrolysis with protease was applied. The use of lysozyme to extract selenium speciesprovided a better efficiency in the total selenium content (higher than 96%), compared to a mechanical disruption of the bacterial cell wall. Analysis of the extracts by anionic exchange HPLC-ICP-MS showed a strong influence of incubation time with protease (24, 48 and 72 h) on selenium chromatographic profile. The results showed that selenocysteine (SeCys) was the only Se species identified in both bacteria representing an 80% of total selenium accumulated. The confirmation of the identity of this Se species was carried out after performing a carbamidomethylation process, prior to enzymatic hydrolysis, and analyzing the extract by reversed phase HPLCESI- MS/MS. These analyses confirmed the presence of SeCys, and no relevant differences were found between the metabolic pathway of both forms of selenium. Moreover, the growth of both bacterial species in the presence of selenite resulted in the formation of biogenic SeNPs. Characterization by TEM before and after their separation from the culture medium showed spherical and monodispersed nanoparticles with an average size (155 ± 19) nm and (172 ± 20) nm for E. coli and S. aureus, respectively. Analysis by spICP-MS showed no significant differences in size with respect to TEM after considering the ionization efficiency of Se.
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    Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro models
    (Food Chemistry, 2023) Espada-Bernabé, Elena; Moreno Martín, Gustavo; Gómez Gómez, Beatriz; Madrid Albarrán, María Yolanda
    Potential risk of nanoparticles present in food additives should be assessed. Although food-grade additive E171 (TiO2) has been banned by European Union due to the potential occurrence of nanometric TiO2, it is still present in stock products and permitted in other countries. TiO2 (nano)particles from the outer coating of colored chocolate candies were determined by TEM (Transmission Electron Microscopy), ICP-MS (Inductively Coupled Plasma Mass Spectrometry) and spICP-MS (Single-Particle-ICP-MS). Total titanium content was in the range of 1219 ± 83 μg/g, except for brown and white candies. Percentage of TiO2 particles bellow 100 nm was under 25% regardless the color, with most frequent size between 120 and 160 nm. In vitro gastrointestinal assays reveal differences in bioaccessibility percentages between whole candy (14%) or aqueous extracts of the coating of candy (37%). More than 90% of bioaccessible titanium was found in particulate form. Caco-2 cells viability decreased around 65% after 24 h exposed to intestinal fraction.
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    Characterization of AgNPs and AuNPs in sewage sludge by single particle inductively coupled plasma-mass spectrometry
    (Talanta, 2022) Moreno Martín, Gustavo; Gómez Gómez, Beatriz; León González, María Eugenia De; Madrid Albarrán, María Yolanda
    This study develops for the first time an analytical method for the characterization of silver and gold nanoparticles in sewage sludge. The evaluation of the effect of temperature, extracting agent and centrifugation speed and time on the extraction yield was carried out through a multifactorial analysis of variance which allows us to select 289 g, 5 min and 20 mM sodium pyrophosphate tetrabasic as optimal extraction conditions. Under these conditions, the analysis of the extract by single particle inductively coupled plasma-mass spectrometry provided recovery percentages of 70 ± 2% and 56 ± 1% for silver and gold nanoparticles, respectively. Moreover, the complementary results obtained upon analysis of these extracts by transmission electron microscopy and single particle inductively coupled plasma-mass spectrometry showed that the developed method did not modify the original size and shape of these nanoparticles during the extraction procedure. Size detection limits of 23 nm and 16 nm as well as number concentration limits of 3.12 × 109 particles kg−1 and 1.38 × 109 particles kg−1 were obtained for silver and gold nanoparticles, respectively. Moreover, a stability study of silver and gold nanoparticles in sewage sludge for 12 months showed differences between the two nanoparticle types. Although the sizes were not affected during the 12 months, silver nanoparticles underwent an oxidation process from 6 months onwards, which was reflected in an increase in the percentage of ionic silver from 14 ± 1% at 6 months to 24 ± 2% at 12 months. The developed methodology represents a simple, reliable and fast tool for detecting, quantifying and assessing the stability of nanoparticles in an important environmental sample such as sewage sludge.
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    Project number: PIMCD315/23-24
    Wooclap-eurekas: participar y aprender tiene recompensa (WE-PARE)
    (2024) Rodríguez Rodríguez, Elena; Sánchez-Paniagua López, Marta; Moreno Guzmán, María; Ojeda Fernández, Irene; Mateos-Aparicio Cediel, Inmaculada; García Alonso, María Alejandra; Pérez Rodríguez, María Luisa; Raggio Quílez, José; Moreno Martín, Gustavo; Gómez Gómez, Beatriz; Sanz Landaluze, Jon; Rueda Rodríguez, María del Carmen; Rupérez García, Alicia; Gallo Orive, Álvaro; Jiménez de la Peña Armada, Rocío Teresa; Rodríguez Moreno, María Pilar
    En el Espacio Europeo de Educación Superior (EEES) se incluye la necesidad de impulsar una docencia más eficiente, en la que se refuerce la capacidad de trabajo autónomo del estudiantado y en la que se fomente su participación activa. Durante el curso 2023/24 se ha realizado un proyecto de innovación docente (PID-WE-PARE), cuyo objetivo principal ha sido fomentar el aprendizaje activo y colaborativo y la mejora de la participación del alumnado en el aula mediante varias estrategias, entre las que se encuentra el uso de Wooclap. En el proyecto se han incluido 825 estudiantes de 3 Grados diferentes (Farmacia, Ciencia y Tecnología de Alimentos-CYTA- y Química) de la Universidad Complutense de Madrid. Se han incluido 7 asignaturas diferentes, 3 de Farmacia, 2 de CYTA y 2 de Química. Se han llevado a cabo diferentes actividades, siendo el uso de Wooclap común en todas las asignaturas. Esta herramienta es una plataforma online gratuita e integrada en Moodle, que permite realizar presentaciones interactivas y plantear preguntas que los estudiantes contestan mediante dispositivos electrónicos, de forma síncrona o asíncrona. Los resultados obtenidos en los Wooclap y otras actividades realizadas (tareas de ejercicios numéricos o casos prácticos a través del campus virtual, socratives, escapes rooms virtuales, etc.) se han usado para otorgar puntos, denominados “eurekas”, a los estudiantes, que al final de curso se han canjeado por diferentes recompensas (material de papelería, visitas a laboratorios y centros de investigación, etc.). Para conocer la opinión de los estudiantes con respecto al proyecto realizado, se pasó una encuesta tipo Likert.