Person:
Goyache Goñi, Joaquín

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First Name
Joaquín
Last Name
Goyache Goñi
URI
https://hdl.handle.net/20.500.14352/80624
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
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2011 - 20203
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Author: García Benzaquén, Nerea × Subject: 636.09 ×

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Now showing 1 - 3 of 3
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    Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
    (Frontiers in Veterinary Science, 2020) García Benzaquén, Nerea; Marta Hernández; Maialen Gutierrez-Boada; Antonio Valero; Navarro Gómez, Alejandro; Milagros Muñoz-Chimeno; Fernández Manzano, Álvaro; Franco Matías Escobar; Martínez Alares, Irene; Bárcena Asensio, María Carmen; González Domínguez, Sergio; Ana Avellón; Jose M. Eiros; Gislaine Fongaro; Domínguez Rodríguez, Lucas José; Goyache Goñi, Joaquín; David Rodríguez-Lázaro
    Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription realtime PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.
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    Management of an outbreak of brucellosis due to B. melitensis in dairy cattle in Spain
    (Research in Veterinary Science, 2011) Álvarez Sánchez, Julio; Sáez, Jose Luis; García Benzaquén, Nerea; Serrat, Carles; Pérez Sancho, Marta; González Domínguez, Sergio; Ortega, Maria Jesús; Gou, Josep; Carbajo, Lucio; Garrido, Fulgencio; Goyache Goñi, Joaquín; Domínguez Rodríguez, Lucas José
    Brucella melitensis is a major human and animal pathogen, with a wide host range that includes all domestic ruminant species, although small ruminants are its preferred hosts. Outbreaks in cattle due to B. melitensis have become a worldwide emerging problem particularly difficult to control due to the lack of knowledge on the epidemiology in this host species and of an effective vaccine. However, combination of molecular tools and strict biosecurity measures can help to solve these difficulties and eventually eradicate the disease from infected herds. In the present report, management of an outbreak in Spain involving four farms, more than 2000 cattle and several human cases is described. Application of Multiple Locus VNTR Analysis (MLVA) allowed identifying the most likely source of infection. Stamping out and test-and-slaughter strategies were applied, proving their usefulness to control the outbreak depending on infection level, and without the need of other alternative measures.
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    Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples
    (Research in Veterinary Science, 2013) Pérez Sancho, Marta; García-Seco Romero, María Teresa; Arrogante, L; García, N; García Benzaquén, Nerea; Martínez Alares, Irene; Díez Guerrier, Alberto Antoine; Perales, A; Goyache Goñi, Joaquín; Domínguez Rodríguez, Lucas José; Álvarez Sánchez, Julio
    DNA-based methods have emerged as an additional tool for Brucella infection-confirmation at a herd level. However, their implementation may require the use of specialized equipment. In this context the recently developed loop-mediated isothermal amplification (LAMP) technique may constitute an additional and cost-effective tool for rapid and specific DNA detection, especially in low income areas. In the present study the usefulness of a newly developed LAMP assay aiming at the multicopy-IS711 sequence was assessed on a variety of clinical samples (n = 81 from abortions and ewes; cattle, n = 3; swine, n = 4) that were analyzed in parallel using real-time PCR and bacteriology. Although overall sensitivities obtained with the three methods were comparable (p > 0.05), our results highlighted the complementarity between bacteriology and molecular-based methods for increased sensitivity. Significant differences (p < 0.05) were observed with all techniques depending on the nature of the sample. Our results demonstrate the potential of the IS711-LAMP technique for direct Brucella detection.