Person:
Hernández Sánchez, María

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First Name
María
Last Name
Hernández Sánchez
URI
https://hdl.handle.net/20.500.14352/86320
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Bioquímica y Biología Molecular
Area
Bioquímica y Biología Molecular
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    High-Throughput CRISPR Screening in Hematological Neoplasms
    (Cancers, 2022) Ancos-Pintado, Raquel; Bragado-García, Irene; Morales, María Luz; García-Vicente, Roberto; Arroyo-Barea, Andrés; Rodríguez-García, Alba; Martínez López, Joaquín; Linares Gómez, María; Hernández Sánchez, María
    CRISPR is becoming an indispensable tool in biological research, revolutionizing diverse fields of medical research and biotechnology. In the last few years, several CRISPR-based genome-targeting tools have been translated for the study of hematological neoplasms. However, there is a lack of reviews focused on the wide uses of this technology in hematology. Therefore, in this review, we summarize the main CRISPR-based approaches of high throughput screenings applied to this field. Here we explain several libraries and algorithms for analysis of CRISPR screens used in hematology, accompanied by the most relevant databases. Moreover, we focus on (1) the identification of novel modulator genes of drug resistance and efficacy, which could anticipate relapses in patients and (2) new therapeutic targets and synthetic lethal interactions. We also discuss the approaches to uncover novel biomarkers of malignant transformations and immune evasion mechanisms. We explain the current literature in the most common lymphoid and myeloid neoplasms using this tool. Then, we conclude with future directions, highlighting the importance of further gene candidate validation and the integration and harmonization of the data from CRISPR screening approaches.