Person: Cruz López, Fátima
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First Name
Fátima
Last Name
Cruz López
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Area
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4 results
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Now showing 1 - 4 of 4
- Project number: 219
Item Infequus: plataforma de enfermedades infecciosas equinas(2019) Juan Ferré, Lucía De; Briones Dieste, Víctor; Bezos Garrido, Javier; Fores Jackson, Paloma; Camino Gutiérrez, Eliazar; Buendía Andrés, Aranzazu; Dorrego Rodríguez, Abel; Cruz López, Fátima; González Domínguez, Sergio; Romero Martínez, Beatriz; García Benzaquén, Nerea; Bárcena Asensio, Carmen; Mazariegos Martínez-Peñalver, María; Ancochea Nodal, Carlos; Hernández Carrillo, Javier; Pérez Sancho, Marta; Pérez Sancho, MartaDesarrollo de la herramienta de formación online Infequus, que ofrecerá a los alumnos y profesionales información actualizada sobre el diagnóstico y control de las enfermedades infecciosas en la especie equina. Item Neuropathological lesions in intravenous BCG-stimulated K18-hACE2 mice challenged with SARS-CoV-2(Veterinary Research, 2024) Sánchez Morales, Lidia; Porras, Néstor; García-Seco Romero, María Teresa; Pérez Sancho, Marta; Cruz López, Fátima; Chinchilla, Blanca; Barroso Arévalo, Sandra; Diaz Frutos, Marta; Buendía, Aránzazu; Moreno, Inmaculada; Briones Dieste, Víctor; Risalde, María de los Ángeles; de la Fuente, José; Juste, Ramón; Garrido, Joseba; Balseiro, Ana; Gortázar, Christian; Rodríguez Bertos, Antonio Manuel; Domínguez, Mercedes; Domínguez Rodríguez, Lucas JoséIn the wake of the COVID-19 pandemic caused by SARS-CoV-2, questions emerged about the potential effects of Bacillus Calmette-Guérin (BCG) vaccine on the immune response to SARS-CoV-2 infection, including the neurodegenerative diseases it may contribute to. To explore this, an experimental study was carried out in BCG-stimulated and non-stimulated k18-hACE2 mice challenged with SARS-CoV-2. Viral loads in tissues determined by RT-qPCR, histopathology in brain and lungs, immunohistochemical study in brain (IHC) as well as mortality rates, clinical signs and plasma inflammatory and coagulation biomarkers were assessed. Our results showed BCG-SARS-CoV-2 challenged mice presented higher viral loads in the brain and an increased frequency of neuroinvasion, with the greatest differences observed between groups at 3–4 days post-infection (dpi). Histopathological examination showed a higher severity of brain lesions in BCG-SARS-CoV-2 challenged mice, mainly consisting of neuroinflammation, increased glial cell population and neuronal degeneration, from 5 dpi onwards. This group also presented higher interstitial pneumonia and vascular thrombosis in lungs (3–4 dpi), BCG-SARS-CoV-2 mice showed higher values for TNF-α and D-dimer values, while iNOS values were higher in SARS-CoV-2 mice at 3–4 dpi. Results presented in this study indicate that BCG stimulation could have intensified the inflammatory and neurodegenerative lesions promoting virus neuroinvasion and dissemination in this experimental model. Although k18-hACE2 mice show higher hACE2 expression and neurodissemination, this study suggests that, although the benefits of BCG on enhancing heterologous protection against pathogens and tumour cells have been broadly demonstrated, potential adverse outcomes due to the non-specific effects of BCG should be considered.- Project number: 371
Item Infequus 2.0: Plataforma de enfermedades infecciosas en équidos(2022) Juan Ferré, Lucía De; Cruz López, Fátima; Álvarez Sánchez, Julio; Ancochea Nodal, Carlos; Bezos Garrido, Javier; Briones Dieste, Víctor; García Benzaquén, Nerea; González Domínguez, Sergio; Hernández Carrillo, Javier; Pérez Sancho, Marta; Rodríguez Bertos, Antonio Manuel; Romero Martínez, Beatriz; Santiago Llorente, Isabel; Domínguez Rodríguez, Lucas José Item Neuropathological lesions in intravenous BCG-stimulated K18-hACE2 mice challenged with SARS-CoV-2(Veterinary Research, 2024) Sánchez Morales, Lidia; Porras González, Néstor; García-Seco Romero, María Teresa; Pérez Sancho, Marta; Cruz López, Fátima; Chinchilla Rodríguez, Blanca; Barroso Arévalo, Sandra; Díaz Frutos, Marta; Buendía Andrés, Aránzazu; Moreno, Inmaculada; Briones Dieste, Víctor; Risalde, María A.; De la Fuente, José; Juste, Ramón; Garrido, Joseba; Balseiro, Ana; Gortázar, Christian; Rodríguez Bertos, Antonio Manuel; Domínguez, Mercedes; Domínguez Rodríguez, Lucas JoséIn the wake of the COVID-19 pandemic caused by SARS-CoV-2, questions emerged about the potential effects of Bacillus Calmette-Guérin (BCG) vaccine on the immune response to SARS-CoV-2 infection, including the neurodegenerative diseases it may contribute to. To explore this, an experimental study was carried out in BCG-stimulated and non-stimulated k18-hACE2 mice challenged with SARS-CoV-2. Viral loads in tissues determined by RT-qPCR, histopathology in brain and lungs, immunohistochemical study in brain (IHC) as well as mortality rates, clinical signs and plasma inflammatory and coagulation biomarkers were assessed. Our results showed BCG-SARS-CoV-2 challenged mice presented higher viral loads in the brain and an increased frequency of neuroinvasion, with the greatest differences observed between groups at 3–4 days post-infection (dpi). Histopathological examination showed a higher severity of brain lesions in BCG-SARS-CoV-2 challenged mice, mainly consisting of neuroinflammation, increased glial cell population and neuronal degeneration, from 5 dpi onwards. This group also presented higher interstitial pneumonia and vascular thrombosis in lungs (3–4 dpi), BCG-SARS-CoV-2 mice showed higher values for TNF-α and D-dimer values, while iNOS values were higher in SARS-CoV-2 mice at 3–4 dpi. Results presented in this study indicate that BCG stimulation could have intensified the inflammatory and neurodegenerative lesions promoting virus neuroinvasion and dissemination in this experimental model. Although k18-hACE2 mice show higher hACE2 expression and neurodissemination, this study suggests that, although the benefits of BCG on enhancing heterologous protection against pathogens and tumour cells have been broadly demonstrated, potential adverse outcomes due to the non-specific effects of BCG should be considered.