Person:
Bonnin Arias, Cristina Natalia

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First Name
Cristina Natalia
Last Name
Bonnin Arias
URI
https://hdl.handle.net/20.500.14352/79903
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
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Author: Bonnin Arias, Cristina Natalia × Author: García Calavia, Marta × Author: Guerrera, María Cristina × Item Type: Publication ×

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Now showing 1 - 2 of 2
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    Light regulates the expression of the BDNF/TrkB system in the adult Zebrafish retina
    (Microscopy Research and Technique, 2012) Guerrera, María Cristina; García Calavia, Marta; Chamorro Gutiérrez, Eva; Montalbano, Giuseppe; López Velasco, Salvador; López Muñiz, Alfonso Joaquín; Germanà, Antonino; Vega Álvarez, José Antonio; Sánchez Ramos, Celia; Bonnin Arias, Cristina Natalia
    The retina of the adult zebrafish express brain‐derived neurotrophic factor (BDNF) and its signaling receptor TrkB. This functional system is involved in the biology of the vertebrate retina and its expression is regulated by light. This study was designed to investigate the effects of cyclic (12 h light/12 h darkness) or continuous (24 h) exposure during 10 days to white light, white‐blue light, and blue light, as well as of darkness, on the expression of BDNF and TrkB in the retina. BDNF and TrkB were assessed in the retina of adult zebrafish using quantitative real‐time polymerase chain reaction and immunohistochemistry. Exposure to white, white‐blue, and blue light causes a decrease of BDNF mRNA and of BDNF immunostaining, independently of the pattern of light exposition. Conversely, in the same experimental conditions, the expression of TrkB mRNA was upregulated and TrkB immunostaining increased. Exposition to darkness diminished BDNF and TrkB mRNAs, and abolished the immunostaining for BDNF but not modified that for TrkB. These results demonstrate the regulation of BDNF and TrkB by light in the retina of adult zebrafish and might contribute to explain some aspects of the complex pathophysiology of light‐induced retinopathies.
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    Expression of TRPV4 in the zebrafish retina during development
    (Microscopy Research and Technique, 2012) Guerrera, María Cristina; García Calavia, Marta; Laurà, Rosaria; Germanà, Antonino; Vega Álvarez, José Antonio; Sánchez Ramos, Celia; Bonnin Arias, Cristina Natalia
    The transient receptor potential (TRP) channels are involved in sensing mechanical/physical stimuli such as temperature, light, pressure, as well as chemical stimuli. Some TRP channels are present in the vertebrate retina, and the occurrence of the multifunctional channel TRP vanilloid 4 (TRPV4) has been reported in adult zebrafish. Here, we investigate the expression and distribution of TRPV4 in the retina of zebrafish during development using polymerase chain reaction (PCR), Western blot, and immunohistochemistry from 3 days post fertilization (dpf) until 100 dpf. TRPV4 was detected at the mRNA and protein levels in the eye of zebrafish at all ages sampled. Immunohistochemistry revealed the presence of TRPV4 in a population of the retinal cells identified as amacrine cells on the basis of their morphology and localization within the retina, as well as the co‐localization of TRPV4 with calretinin. TRPV4 was first (3 dpf) found in the soma of cells localized in the inner nuclear and ganglion cell layers, and thereafter (10 dpf) also in the inner plexiform layer. The adult pattern of TRPV4 expression was achieved by 40 dpf the expression being restricted to the soma of some cells in the inner nuclear layer and ganglion cell layers. These data demonstrate the occurrence and developmental changes in the expression and localization of TRPV4 in the retina of zebrafish, and suggest a role of TRPV4 in the visual processing.