Person:
Bonnin Arias, Cristina Natalia

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First Name
Cristina Natalia
Last Name
Bonnin Arias
URI
https://hdl.handle.net/20.500.14352/79903
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
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Author: Bonnin Arias, Cristina Natalia × Author: García Calavia, Marta × Item Type: Publication × Subject: 576:611.843 ×

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    Expression of TRPV4 in the zebrafish retina during development
    (Microscopy Research and Technique, 2012) Guerrera, María Cristina; García Calavia, Marta; Laurà, Rosaria; Germanà, Antonino; Vega Álvarez, José Antonio; Sánchez Ramos, Celia; Bonnin Arias, Cristina Natalia
    The transient receptor potential (TRP) channels are involved in sensing mechanical/physical stimuli such as temperature, light, pressure, as well as chemical stimuli. Some TRP channels are present in the vertebrate retina, and the occurrence of the multifunctional channel TRP vanilloid 4 (TRPV4) has been reported in adult zebrafish. Here, we investigate the expression and distribution of TRPV4 in the retina of zebrafish during development using polymerase chain reaction (PCR), Western blot, and immunohistochemistry from 3 days post fertilization (dpf) until 100 dpf. TRPV4 was detected at the mRNA and protein levels in the eye of zebrafish at all ages sampled. Immunohistochemistry revealed the presence of TRPV4 in a population of the retinal cells identified as amacrine cells on the basis of their morphology and localization within the retina, as well as the co‐localization of TRPV4 with calretinin. TRPV4 was first (3 dpf) found in the soma of cells localized in the inner nuclear and ganglion cell layers, and thereafter (10 dpf) also in the inner plexiform layer. The adult pattern of TRPV4 expression was achieved by 40 dpf the expression being restricted to the soma of some cells in the inner nuclear layer and ganglion cell layers. These data demonstrate the occurrence and developmental changes in the expression and localization of TRPV4 in the retina of zebrafish, and suggest a role of TRPV4 in the visual processing.