Person: Bonnin Arias, Cristina Natalia
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First Name
Cristina Natalia
Last Name
Bonnin Arias
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
Identifiers
7 results
Search Results
Now showing 1 - 7 of 7
Item Acid-Sensing ion channels 2 and 4 (Asic 2 Saic 4) are regulated by light in the zebrafish retina(2012) Sánchez Ramos, Celia; Germaná, A.; Bonnin Arias, Cristina Natalia; Chamorro Gutiérrez, E.; Navarro Valls, J.J.; García Ortega, M.; Pérez Carrasco, María Jesús; Vega, J.A.Purpose: Acid-sensing ion channels (ASICs) are H+- gated cation channels that monitor deviations from the physiological values of extracellular pH. ASIC genes in zebrafish (zASICs) are expressed in the central nervous system and the retina. pH variations in the retina are thought to be involved in the fine-tunning of visual perception and in the adaptation of the retinal responses to different light-induced retinal degeneration. This study examines the effects of continuous light or darkness exposure in the mRNA levels and cell distribution of ASIC2 and ASIC4 in the retina of adult zebrafish.Item New Optical Filter Plate for Use as Eye Protection by Welders(Welding journal, 2013) Langa Moraga, Antonio; Bonnin Arias, Cristina Natalia; Chamorro, Eva; Pérez Carrasco, María Jesús; Sánchez Ramos, CeliaPeople whose work tasks involve the use of welding torches are at special risk of suffering eye injuries due to the emission of visible, short-wavelength radiation. Current legislation requires that a company provide its employees with protection against the harmful radiation produced by welding equipment. Often, however, a worker will be reluctant to use protective goggles since these markedly cut visibility and can consequently lead to errors or even burns. This practice of avoiding the use of protection makes them susceptible to suffer irreversible severe retinal damage leading to partial or complete loss of vision. In this paper, we propose the use of a new photoprotective filter in the form of safety goggles that seeks to improve the compromised vision produced by conventional filters. We compare a series of visual function variables in 36 adults, aged 30 to 58 years, using the new optical filter and a conventional filter used for welding protection. Our findings suggest that the filter proposed provides optimal protection against the harmful effects of short-wavelength radiation while minimizing the reduced vision effects of conventional filters used for this purpose.Item Effects of Light‐emitting Diode Radiations on Human Retinal Pigment Epithelial Cells in vitro(Photochemistry and Photobiology, 2012) Chamorro, Eva; Muñoz de Luna, Javier; Bonnin Arias, Cristina Natalia; Pérez Carrasco, María Jesús; Vázquez Molini, Daniel; Sánchez Ramos, CeliaHuman visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiations on human retinal pigment epithelial cells (HRPEpiC). They were exposed to three light-darkness (12 h/12 h) cycles, using blue-468 nm, green-525 nm, red-616 nm and white light. Cellular viability of HRPEpiC was evaluated by labeling all nuclei with DAPI; Production of reactive oxygen species (ROS) was determined by H2DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2AX histone activation, and apoptosis was evaluated by caspases-3,-7 activation. It is shown that LED radiations decrease 75-99% cellular viability, and increase 66-89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light-darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPEpiC.Item Differences in the myosis response to increased light intensity using the x-480 filter in persons over 65 years old.(2009) Sánchez Ramos, Celia; Díaz de Cerio, E.R.; Bonnin Arias, Cristina Natalia; Díaz Serrano, Y.; Vinas Pena, M.; Molina Gómez, C.; Pérez Carrasco, María JesúsPURPOSE: To assess the effect of the X-480 filter (Cutoff 480nm) on mesopic and scotopic pupil diameters in persons older than 65 years, we examined 196 eyes of 98 subjects (52 men/46 women) aged 65 to 101 years.Item Changes in the spatial resolution and in the color contrast perception due to interjected face protector screens in the emergency situations in mesopic conditions.(2010) Bonnin Arias, Cristina Natalia; Pérez Carrasco, María Jesús; Chamorro Gutiérrez, E.; Navarro Blanco, Carolina; Gomez Vadillo, T.; Sánchez Ramos, CeliaPURPOSE: Assess the changes in the visual capabilities related to protector masks that are mandatory in emergency situations. The study was carried out in mesopic conditions. METHODS: 36 adults were assessed. Everyone was from both genders, ranging 18 to 49 years old, with an average of 30 +- 8 years old. Face protectors screens have been utilized on Individual Protection Kits for emergency situations. Every measure was conducted by the same examiner and into the same laboratory in mesopic conditions. Visual cuity was evaluated by means of the Sloan´s test at 100% and 5% of contrast in logarithmic units. The contrast Sensitivity with the CVS1000 test and the colour perception with Ishihara´s test (each test was accomplished by the manufacturer´s recommendations). RESULTS: The capacity for distinguishing details in the assessment carried out without a protector screen was of -0.05 +- 0.05 logMAR, and with a protector screen was of 0.00+-0.11 logMAR being the difference statistically significant. Contrast Sensitivty values are lower when a protector filter is interjected, with p-valor 0.0013. Regarding coloru perception significative differences were detected after protector screen application and withdrawal. CONCLUSION: In mesopic ocnditions, spatial resolution, contrast sensitivity and colour perception are signifcantly more decreased due to face protector needed for emergency situations.Item Analysis of intra-observer´s repeatability in retinopathy´s classification of patients with, both, clear and yellow IOL´S implantation.(2009) Pérez Carrasco, María Jesús; Sánchez Ramos, Celia; Vinas Pena, M.; Bonnin Arias, Cristina Natalia; Forlan, A.; San Miguel, M.; Ramírez, G.PURPOSE: to discriminate between different moderate retinopathies according to the clinical Age-Related Maculopathy Staging System (CARMS) using for it retinographies. Also to analyze de intra-observer`s reliability. METHODS: 808 retinographies were made to 101 subjects (both gender). Every patient had suffered cataract surgery with clear and yellow IOL´s implantation, 55 retinographies were classified by using CARMS system, which categorizes the images depending on the Drusen´s presence or absense, retinal pigment epithelium irregularities, geographic atrophy, pigment epithelium detachment and choroidal neovascularisation. The 55 retinographies categorizing was made by 2 experts in a blind, independent way. The method followed consisted in 2 different sequences with every of the 3 evaluated systems. The goal of the doubled-classification-method, with changing order no randomised, was to reject the influence of the evaluation of the previous image in the following one (Velo´s effect). Inter-observer´s repetability was checked, because of Kappa index was calculated, as an agreement parameter between both Experts. RESULTS: Intra-observer´s reliability analysis was the following: CARMS concondance obtained was 87.27% for observer A and 95.45% for the observer B. The expected concordance was 57.36% and 57044% for A and B respectively. Kappa index was 0.7016 and 008932 for A and B respectively. Drusen`s concordance obtained for observer A eas 87.27% and 98.18% for observer B. The expeted values were 59050% and 61.16% for A and B respectively. Kappa index was 0.6857 and 0.9532 for A and B respectively. Pigmentation´s concordance obteined was 87027% and 92.73%, for A and B observers. The expected concordance was 55021% and 53.72% for A and B and Kappa indexes were 0.7159 and 0.8429 for both. The reliability measured by Kappa index followed the rule: 0.80-1.00 (Excellent), 0.60-0.80 (Good), 0.40-0.60 (Moderate), 0.20-0.40 (Low), <0.20 (Bad). CONCLUSIONS: Kappa´s indexes obteined for the intra-observer analysis show a good reliability, between Godd and Excellent. A higher discrepancy in the firs retinas, which were classified, was observed. This means that a previous trainins is necessary.Item Short-term variations in macular thickness in subjects implanted with a clear vs yellow intraocular lens.(2011) Chamorro Gutiérrez, E.; Bonnin Arias, Cristina Natalia; Aguirre Vila Coro, V.; Pérez Carrasco, María Jesús; Álvarez Rementeria, L.; Armandá maresca, F.; Sánchez Ramos, CeliaPURPOSE: To examine by optical coherence tomography (OCT) macular thickness variations produced over time in psudophakic subjects with a clear introcular lens (IOL) in one eye and a yellow IOL in the follow eye. MATERIAL AND METHODS: Macular thickness measurements were obtained in 17 subjects over the age of 65 years who had undergone cataract surgery in both eyes and had been implanted with different IOLs (clear and yellow) in two separate surgeries. Macular thickness was determined using the instruments StratusOCT and CirrusOCT (Carl Zeiss Meditec, Inc.) in 3 sessions each separated by a 2-years gap. RESULTS: Significant differences in macular thicness measurements were detected for the two types of IOL. After 4 years of follow-up macular thicknesses were significantly greater in the eyes implanted with a yellow IOL than in the fellow eyes with a clear IOL. CONCLUSIONS: Our findings indicate that the use fo a blue-light filtering IOL protects the eye from or delays retinal thinning.