Person:
Bonnin Arias, Cristina Natalia

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First Name
Cristina Natalia
Last Name
Bonnin Arias
URI
https://hdl.handle.net/20.500.14352/79903
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
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Author: Sánchez Ramos, Celia × End date: 2012 × Start date: 2012 × Item Type: Publication × Has files: true × Subject: 617.7-001.15 ×

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    Effects of Light‐emitting Diode Radiations on Human Retinal Pigment Epithelial Cells in vitro
    (Photochemistry and Photobiology, 2012) Chamorro, Eva; Muñoz de Luna, Javier; Bonnin Arias, Cristina Natalia; Pérez Carrasco, María Jesús; Vázquez Molini, Daniel; Sánchez Ramos, Celia
    Human visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiations on human retinal pigment epithelial cells (HRPEpiC). They were exposed to three light-darkness (12 h/12 h) cycles, using blue-468 nm, green-525 nm, red-616 nm and white light. Cellular viability of HRPEpiC was evaluated by labeling all nuclei with DAPI; Production of reactive oxygen species (ROS) was determined by H2DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2AX histone activation, and apoptosis was evaluated by caspases-3,-7 activation. It is shown that LED radiations decrease 75-99% cellular viability, and increase 66-89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light-darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPEpiC.
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    Riesgos personales producidos por LEDs utilizados en dispositivos de uso cotidiano
    (Seguridad y medio ambiente (Fundación MAPFRE), 2012) Chamorro Gutiérrez, Eva; Bonnin Arias, Cristina Natalia; Lobato Rincón, Luis Lucio; Navarro Valls, Juan José; Ramirez Mercado, Guillermo; Navarro Blanco, Carolina; Sánchez Ramos, Celia
    El uso de fuentes de iluminación LED (diodos emisores de luz) está creciendo de manera exponencial tanto en el campo de la iluminación ambiente como en dispositivos de uso personal y doméstico como smartphones, pantallas de ordenador, electrodomésticos, etc. Sin embargo, el principal problema que plantean los LEDs que emiten luz blanca radica en su alto contenido de radiaciones de la banda del azul, que son dañinas para el sistema visual. En este proyecto se ha diseñado un dispositivo de iluminación formado por diodos LED de diferentes características espectrales para comprobar si producen daño en la retina, sobre todo en células del epitelio pigmentario. Los experimentos han demostrado que la exposición a la luz aumenta el porcentaje de muerte celular inducida por la luz para todas las fuentes de luz LED, especialmente en las células expuestas a luz azul y blanca, en las que se produjo un aumento de la muerte celular respecto al control del 92% y 94% respectivamente. El estudio concluye que la exposición a altas intensidades de luz LED durante ciclos de luz/oscuridad produce daños en las células de la retina.