Person:
Bonnin Arias, Cristina Natalia

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First Name
Cristina Natalia
Last Name
Bonnin Arias
URI
https://hdl.handle.net/20.500.14352/79903
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Óptica y Optometría
Department
Optometría y Visión
Area
Optica
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2012 - 20192
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Author: Sánchez Ramos, Celia × Item Type: Publication × Subject: 617.7-001.15 × Subject: 617.735-001.15 ×

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    Effects of Light‐emitting Diode Radiations on Human Retinal Pigment Epithelial Cells in vitro
    (Photochemistry and Photobiology, 2012) Chamorro, Eva; Muñoz de Luna, Javier; Bonnin Arias, Cristina Natalia; Pérez Carrasco, María Jesús; Vázquez Molini, Daniel; Sánchez Ramos, Celia
    Human visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiations on human retinal pigment epithelial cells (HRPEpiC). They were exposed to three light-darkness (12 h/12 h) cycles, using blue-468 nm, green-525 nm, red-616 nm and white light. Cellular viability of HRPEpiC was evaluated by labeling all nuclei with DAPI; Production of reactive oxygen species (ROS) was determined by H2DCFDA staining; mitochondrial membrane potential was quantified by TMRM staining; DNA damage was determined by H2AX histone activation, and apoptosis was evaluated by caspases-3,-7 activation. It is shown that LED radiations decrease 75-99% cellular viability, and increase 66-89% cellular apoptosis. They also increase ROS production and DNA damage. Fluorescence intensity of apoptosis was 3.7% in nonirradiated cells and 88.8%, 86.1%, 83.9% and 65.5% in cells exposed to white, blue, green or red light, respectively. This study indicates three light-darkness (12 h/12 h) cycles of exposure to LED lighting affect in vitro HRPEpiC.
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    Efecto de la luz: Alternativas de protección ante la luz azul
    (Franja visual, 2019) Bonnin Arias, Cristina Natalia; Gutiérrez Jorrín, Sara.; Rodríguez Alonso, Xabier; Sánchez Ramos, Celia
    La luz azul comprende la radiación luminosa entre 380 y 500 nm y constituye la banda más energética del espectro visible. La luz azul, además de ser emitida por el sol con altas intensidades, es la base de la luz LED emitida por las pantallas de todo tipo de dispositivos electrónicos utilizados actualmente (smartphones, tablets, ordenadores…). Esta iluminación LED fomenta el cansancio visual, dificultando la visión, produciendo resequedad en los ojos y dolores de cabeza, llegando, incluso, a alterar los ciclos circadianos. Además, numerosas investigaciones han demostrado que, dentro del espectro visible, la luz azul es la más dañina para la retina,pudiendo desembocar, finalmente, en una degeneración macular precoz.