Person: Sevane Fernández, Natalia
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First Name
Natalia
Last Name
Sevane Fernández
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Producción Animal
Area
Producción Animal
Identifiers
16 results
Search Results
Now showing 1 - 10 of 16
Item Conservation priorities of Iberoamerican pig breeds and their ancestors based on microsatellite information(Heredity, 2016) Cortés Gardyn, Óscar; Martínez, A. M.; Cañón Ferreras, Francisco Javier; Sevane Fernández, Natalia; Gama, L. T.; Ginja, C.; Landi, V.; Zaragoza, P.; Carolino, N.; Vicente, A.; Sponenberg, P.; Delgado, J. V.; BioPig ConsortiumCriollo pig breeds are descendants from pigs brought to the American continent starting with Columbus second trip in 1493. Pigs currently play a key role in social economy and community cultural identity in Latin America. The aim of this study was to establish conservation priorities among a comprehensive group of Criollo pig breeds based on a set of 24 microsatellite markers and using different criteria. Spain and Portugal pig breeds, wild boar populations of different European geographic origins and commercial pig breeds were included in the analysis as potential genetic influences in the development of Criollo pig breeds. Different methods, differing in the weight given to within- and between-breed genetic variability, were used in order to estimate the contribution of each breed to global genetic diversity. As expected, the partial contribution to total heterozygosity gave high priority to Criollo pig breeds, whereas Weitzman procedures prioritized Iberian Peninsula breeds. With the combined within- and between-breed approaches, different conservation priorities were achieved. The Core Set methodologies highly prioritized Criollo pig breeds (Cr. Boliviano, Cr. Pacifico, Cr. Cubano and Cr. Guadalupe). However, weighing the between- and within-breed components with FST and 1-FST, respectively, resulted in higher contributions of Iberian breeds. In spite of the different conservation priorities according to the methodology used, other factors in addition to genetic information also need to be considered in conservation programmes, such as the economic, cultural or historical value of the breeds involved.Item New single nucleotide polymorphisms in Alectoris identified using chicken genome information allow Alectoris introgression detection(Molecular Ecology Resources, 2010) Sevane Fernández, Natalia; Cortés Gardyn, Óscar; García D; Cañón Ferreras, Francisco Javier; Dunner Boxberger, Helene SusanaUsing the chicken genome, 114 polymorphisms (109 SNPs and 5 INDELs) were identified in the Alectoris genus by polymerase chain reaction–single strand conformation polymorphism. Using these, a panel of SNPs is described, which allows easy detection of introgression of Alectoris chukar in wild Alectoris rufa populations, when used with a primer extension protocol. The selected polymorphisms were genotyped and their allelic frequencies estimated on 98 A. rufa partridges sampled from nonrestocking Spanish areas, and 63 A. chukar partridges from Greek and Spanish farms. Power calculations to determine an optimum subset of markers for a given significance level were performed.Item Syringohydromyelia in Dogs: The Genomic Component Underlying a Complex Neurological Disease(Animals, 2022) Andrino García, Sandra; Lorenzo, Valentina; Dunner Boxberger, Helene Susana; Contreras Alcaide, Elisabeth; Cañón Ferreras, Francisco Javier; Sevane Fernández, NataliaSyringohydromyelia (SHM) is a neurological disorder characterized by the appearance of fluid-containing cavities within the spinal cord. Although SHM is thought to be under multigenic control, the molecular basis of this disease remains poorly defined. A genome-wide association study (GWAS) was carried out comparing the whole genome sequences (WGS) from 12 dogs with SHM and 2 panels of 26 dogs (either older than 5 years and showing the absence of SHM or belonging to breeds not susceptible to SHM) to identify candidate genes associated with the development of SHM. Seven candidate genes were identified. Of these, five genes were determined to be involved in bone development (PLXNA2, HHAT, MBOAT2, ITGAV) and calcium homeostasis (HPCAL1). Although further validation is needed at the transcript level, it is worth highlighting the association of a possible pathogenic variant which generated a new intronic branch-site sequence in PLXNA2 (T/C, CFA7:7043294). Considering previous studies in dogs that show SHM related to craniocervical junction (CCJ) malformations, these genes can be considered good candidates for the development of this disease. This report dissects the genomic component of SHM in dogs, which paves the way for further research on this complex disease found both in canine and human species.Item A novel missense variant in endothelin‐2 ( EDN2 ) causes a growth and respiratory lethal syndrome in bovine(Animal Genetics, 2022) Eusebi, Paulina G.; Cortés Gardyn, Óscar; Contreras, Elisabeth; Cañón Ferreras, Francisco Javier; Dunner Boxberger, Helene Susana; Sevane Fernández, NataliaThe high level of fragmentation of the Spanish Lidia cattle breed, divided into lineages called ‘castas’ and into herds within lineages based on reproductive isolation, increases the risk of homozygosity and the outbreak of recessive genetic defects. Since 2004, an increasing number of calves have been identified in a Lidia herd with signs of severe growth retardation, respiratory alterations and juvenile lethality, which constitutes a novel inherited syndrome in cattle and was subsequently termed growth and respiratory lethal syndrome. We performed a genome-wide association study on a cohort of 13 affected calves and 24 putative non-carrier parents, mapping the disease to a wide 6 cM region on bovine chromosome 3 (p < 10−7). Whole genome re-sequencing of three affected calves and three putative non-carrier parents identified a novel missense variant (c.149G>A|p.Cys50Tyr) in exon 2 of the endothelin 2 (EDN2) gene. Bioinformatic analyses of p.Cys50Tyr effects predicted them to be damaging for both the structure and the function of the edn2 protein, and to create a new site of splicing that may also affect the pattern of pre-mRNA splicing and exon definition. Sanger sequencing of this variant on the rest of the sample set confirmed the segregation pattern obtained with whole genome re-sequencing. The identification of the causative variant and the development of a diagnostic genetic test enable the efficient design of matings to keep the effective population size as high as possible, as well as providing insights into the first EDN2-associated hereditary disease in cattle or other species.Item SNPs included in candidate genes involved in muscle, lipid and energy metabolism behave like neutral markers(Animal Production Science, 2015) Sevane Fernández, Natalia; Cañón Ferreras, Francisco Javier; Williams, John L.; Levéziel, Hubert; Valentini, Alessio; Dunner Boxberger, Helene Susana; GemQual ConsortiumStudies of population structure and diversity in cattle have provided insights into the origins of breeds, their history and evolution, and allow the identification of global livestock diversity hotspots, which is important for conservation of diversity. Genetic diversity, genetic relationship, population structure, and the presence of hotspots of genetic diversity among 15 European bovine breeds from five countries were assessed using 435 single nucleotide polymorphisms (SNP) markers identified in candidate genes for muscle, lipid and energy metabolism, thus providing the opportunity to compare the breed relationships obtained using putatively functional markers with previous data using neutral loci. Individuals belonging to 11 breeds tended to be clearly assigned to a single cluster when the number of pre-defined populations reached a maximum in the likelihood of the data at K = 12, whereas Asturiana de los Valles, Danish Red, Simmental, and Avileña-Negra Ibérica displayed a greater degree of admixture, which may be explained by their diverse ancestry. Although overall results were in agreement with those reported by studies based on neutral genetic variations, some additional breed relationship information emerged using markers in candidate functional loci, including the relationship between the Asturiana de los Valles and Piedmontese, and Danish Red and Charolais breeds. This study indicates that the analysed loci have not been main targets for selection or for adaptation processes, but also that SNP within candidate genes related with beef characteristics and performance may provide a slightly new perspective on past breeding and may also help in the development of strategies for the rational conservation of livestock diversity.Item Polymorphisms in ten candidate genes are associated with conformational and locomotive traits in Spanish Purebreed horses(Journal of Applied Genetics, 2016) Sevane Fernández, Natalia; Dunner Boxberger, Helene Susana; Boado, Ana; Cañón Ferreras, Francisco JavierThe Spanish Purebred horses, also known as Andalusian horses, compete to the highest standards in international dressage events. Gait and conformation could be used as early selection criteria to detect young horses with promising dressage ability. Although the genetic background of equine size variation has been recently uncovered, the genetic basis of horse conformational and locomotive traits is not known, hampered by the complex genetic architecture underlying quantitative traits and the lack of phenotypic data. The aim of this study was to validate the loci associated with size in 144 Spanish Purebred horses, and to seek novel associations between loci previously associated with the development of osteochondrosis (OC) lesions and 20 conformational and locomotive traits. Ten loci were associated with different conformational and locomotive traits (LCORL/NCAPG, HMGA2, USP31, MECR, COL24A1, MGP, FAM184B, PTH1R, KLF3 and SGK1), and the LCORL/NCAPG association with size in the Spanish Purebred horse was validated. Except for HMGA2, all polymorphisms seem to influence both the prevalence of OC lesions and morphological characters, supporting the link between conformation and OC. Also, the implication of most genes in either immune and inflammatory responses and cellular growth, or ossification processes, reinforces the role that these mechanisms have in the aetiology of OC, as well as their reflection on the general conformation of the individual. These polymorphisms could be used in marker-assisted selection (MAS) programmes to improve desirable conformational traits, but taking into account their possible detrimental effect on OC prevalence.Item Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens(Plos One, 2014) Sevane Fernández, Natalia; Bialade, Federica; Velasco Villar, Susana; Rebolé Garrigós, Almudena; Rodríguez Membibre, María Luisa; Ortiz Vera, Luis Tomás; Cañón Ferreras, Francisco Javier; Dunner Boxberger, Helene Susana; te Pas Marinus FWInclusion of prebiotics in the diet is known to be advantageous, with positive influences both on health and growth. The current study investigated the differences in the hepatic transcriptome profiles between chickens supplemented with inulin (a storage carbohydrate found in many plants) and controls. Liver is a major metabolic organ and has been previously reported to be involved in the modification of the lipid metabolism in chickens fed with inulin. A nutrigenomic approach through the analysis of liver RNA hybridized to the Affymetrix GeneChip Chicken Genome Array identified 148 differentially expressed genes among both groups: 104 up-regulated (≥1.4-fold) and 44 down-regulated (≤0.6-fold). Quantitative real-time PCR analysis validated the microarray expression results for five out of seven genes tested. The functional annotation analyses revealed a number of genes, processes and pathways with putative involvement in chicken growth and performance, while reinforcing the immune status of animals, and fostering the production of long chain fatty acids in broilers supplemented with 5 g of inulin kg−1 diet. As far as we are aware, this is the first report of a microarray based gene expression study on the effect of dietary inulin supplementation, supporting further research on the use of this prebiotic on chicken diets as a useful alternative to antibiotics for improving performance and general immunity in poultry farming, along with a healthier meat lipid profile.Item Muscle lipid composition in bulls from 15 European breeds(Livestock Science, 2014) Sevane Fernández, Natalia; Nute G; Sañudo C; Cortés Gardyn, Óscar; Cañón Ferreras, Francisco Javier; Williams JL; Dunner Boxberger, Helene SusanaCattle meat provides essential nutrients necessary for a balanced diet and health preservation. Besides nutritional quality, consumers' preferences are related to specific attributes such as tenderness, taste and flavour. The present study characterizes the fatty acid composition of beef, which is an important factor in both nutritional and quality values, in 15 European cattle breeds fed a similar diet and reared in five countries (United Kingdom, Denmark, France, Italy and Spain). The effect of possible slight differences on diet composition which might have occurred between countries were included in the breed effect which confounds country, diet, slaughter house and slaughter day as all individuals of a same breed were managed simultaneously. The wide range of breeds studied and the significant differences on lipid profile described here provide a broad characterization of beef meat, which allows giving a better response to the variety of consumers' preferences. Regarding meat health benefits, the groups that stand out are: the double-muscled animals, which displayed lower total fat, lower proportion of saturated (SFA) and monounsaturated (MUFA) fatty acids, and a higher proportion of polyunsaturated (PUFA) fatty acids; and Limousin and Charolais breeds with a significantly higher conversion of 18:3n-3 PUFA to the long chain 22:6n-3 PUFA.Item Candidate gene analysis of osteochondrosis in Spanish Purebred horses(Animal Genetics, 2016) Sevane Fernández, Natalia; Dunner Boxberger, Helene Susana; Boado, A.; Cañón Ferreras, Francisco JavierEquine osteochondrosis (OC) is a frequent developmental orthopaedic disease with high economic impact on the equine industry and may lead to premature retirement of the animal as a result of chronic pain and lameness. The genetic background of OC includes different genes affecting several locations; however, these genetic associations have been tested in only one or few populations, lacking the validation in others. The aim of this study was to identify the genetic determinants of OC in the Spanish Purebred horse breed. For that purpose, we used a candidate gene approach to study the association between loci previously implicated in the onset and development of OC in other breeds and different OC locations using radiographic data from 144 individuals belonging to the Spanish Purebred horse breed. Of the 48 polymorphisms analysed, three single nucleotide polymorphisms (SNPs) located in the FAF1, FCN3 and COL1A2 genes were found to be associated with different locations of OC lesions. These data contribute insights into the complex gene networks underlying the multifactorial disease OC, and the associated SNPs could be used in a marker-assisted selection strategy to improve horse health, welfare and competitive lifespan.Item Association of genes involved in carcass and meat quality traits in 15 European bovine breeds(Livestock Science, 2013) Dunner Boxberger, Helene Susana; Sevane Fernández, Natalia; García D; Cortés Gardyn, Óscar; Valentini A; Williams JL; Mangin B; Cañón Ferreras, Francisco Javier; Levéziel HVariations in meat quality traits are under complex genetic control and improvement has been hampered by the difficulty in their measurement. Several QTL have been reported for different meat quality related traits, but few genes have been described which explain large amounts of the phenotypic variation. The use of single nucleotide polymorphism (SNP) marker panels with predictive value for carcass traits have been evaluated for cattle and SNP are commercially available even though their predictive accuracy may be low in different breeds. To identify new molecular markers for meat quality, an association study was performed in 15 breeds of cattle using 389 SNP belonging to 206 candidate genes known to be involved in muscle development, metabolism and structure. Fifty-four SNP belonging to 20 different genes were found associated with different growth, carcass and meat quality traits. Some of them were novel associations and other were replications of known associations. Among the former, the gene-network associated with the calpain/calpastatin system was shown to be associated with meat texture, although small effects are found for the examined polymorphisms. Novel associations also included SNP in AANAT which was associated with collagen (P=0.006), CAST with fatty acid muscle composition (P=0.00003), CYP1A1 with juiciness (P=0.0005), DGAT2 with physical traits (P=0.0009) and lipid content (P=0.01) in muscle, MADH3 with the myofibrilar fragmentation index (MFI) (P=0.01), NEB with weight (P=0.00009), PCSK1 with juiciness (P=0.002), PLOD3 with carcass performance (P=0.0009) and fatty acids (P=0.04), and PGAM2 and VIM with post-mortem maturation (P=0.00008 and 0.000005, respectively). These data provide a starting point to investigate the complex gene-networks underlying economically important traits which are of importance to the beef industry for the improvement of production efficiency and meat quality.