Person:
Raggio Quílez, José

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First Name
José
Last Name
Raggio Quílez
URI
https://hdl.handle.net/20.500.14352/77615
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Farmacología, Farmacognosia y Botánica
Area
Botánica
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20101
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Author: Crespo De Las Casas, Ana María × Subject: 615.212 ×

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    HPLC isolation of antioxidant constituents from Xanthoparmelia spp
    (Journal of Pharmaceutical and Biomedical Analysis, 2010) Amo De Paz, Guillermo; Raggio Quílez, José; Gómez-Serranillos Cuadrado, María Pilar; Palomino Ruiz-Poveda, Olga María; González Burgos, Elena María; Carretero Accame, María Emilia; Crespo De Las Casas, Ana María
    A chromatographic method is described for the purification and characterization of secondary lichen substances with biological activity. A simple reversed-phase high-performance liquid chromatography method with gradient elution has been developed that allows the determination and isolation of salazinic, usnic and stictic acids from lichen samples in a single run and the quantification of every acid in the tested extracts. The antioxidant activity of both the isolated compounds and the respective lichen belonging to Xanthoparmelia genus was determined by the Oxygen Radical Absorbance Capacity (ORAC) assay; their effect as free radical scavengers, effect on cell survival by the 3(4,5-dimethyltiazol-2-yl)-2,5-diphenyltetrazolium reduction assay and 2′,7′-dichlorofluorescin diacetate method were tested on U373 MG human astrocytome cell line. Both lichens extracts and all isolated compounds protected U373 MG cells from hydrogen peroxide-induced damage, suggesting that they could act as antioxidant agents in those neurodegenerative disorders associated with oxidative damage, such as Alzheimer's disease and Parkinson's disease.