Person: Rebollada Merino, Agustín Miguel
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First Name
Agustín Miguel
Last Name
Rebollada Merino
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Medicina y Cirugía Animal
Area
Identifiers
5 results
Search Results
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Item An Outbreak of Aeromonas salmonicida in Juvenile Siberian Sturgeons (Acipenser baerii)(Animals, 2023) Vázquez Fernández, Esther; Chinchilla Rodríguez, Blanca; Rebollada Merino, Agustín Miguel; Domínguez Rodríguez, Lucas José; Rodríguez Bertos, Antonio ManuelAeromonas salmonicida is one of the major threats to world aquaculture, causing fish furunculosis and high mortality rates in cultured fish, particularly salmonids. Although Aeromonas spp. is a thoroughly studied pathogen, little is known regarding aeromoniasis in sturgeons. After a mortality outbreak, four juvenile sturgeons (Acipenser baerii) were submitted for autopsy and tissue samples were collected for histopathological and microbiological studies. The external examination revealed size heterogenicity, skin hyperpigmentation and reduced body condition of sturgeons. Within the abdominal cavity, mild hepatomegaly and splenomegaly were observed, as well as generalized organic congestion. Histology revealed severe multifocal haemorrhagic and ulcerative dermatitis, mainly localized in the dorsal and latero-ventral areas of fish. The histological study also showed moderate to severe inflammation of gills and organic lesions compatible with septicaemia. Bacterial isolates were identified as Aeromonas salmonicida subsp. salmonicida using MALDI-TOF MS and PCR. Overall, the lesions first described here are consistent with those previously reported in other cultured fish species and contribute to a better understanding of the pathogenesis of Aeromonas salmonicida subsp. salmonicida in the Siberian sturgeon, aside from providing new diagnostic tools for bacterial diseases impacting the fast-growing industry of caviar.Item First detection of Flavobacterium psychrophilum in juvenile Siberian sturgeons (Acipenser baerii) and description of the pathological findings(Journal of fish diseases, 2023) Chinchilla Rodríguez, Blanca; Vázquez Fernández, Esther; Rebollada Merino, Agustín Miguel; Pérez Sancho, Marta; Domínguez Rodríguez, Lucas José; Rodríguez Bertos, Antonio ManuelFlavobacterium psychrophilum affects many cultured fish species and is considered one of the most important bacterial pathogens causing substantial economic losses in salmonid aquaculture worldwide. Here, F. psychrophilum was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nested PCR as the aetiological agent causing mortality in diseased juvenile Siberian sturgeons (Acipenser baerii) reared on a freshwater fish farm. Diseased sturgeons were lethargic and displayed dark skin pigmentation, increased mucus production and the presence of skin ulcerations and haemorrhages specially on the ventral side and the base of fins. The histological examination of fish revealed proliferative branchitis, ulcerative and necrotizing dermatitis and myositis, lymphoid tissue atrophy, liver and kidney degeneration and thrombosis. To the best of our knowledge, this is the first report describing the infection of Siberian sturgeons by F. psychrophilum. The detection of F. psychrophilum in diseased Siberian sturgeons and the description of the pathological findings observed during the outbreak may contribute to a better understanding of the bacterium pathogenicity and the range of fish species susceptible to infection.Item Forensic investigations of religious rituals involving poultry: a case report(Journal of Comparative Pathology, 2023) Vázquez Fernández, Esther; Rebollada Merino, Agustín Miguel; Chinchilla Rodríguez, Blanca; Porras González, Néstor; Rodríguez Bertos, Antonio ManuelAnimal victims of human cruelty are receiving increasing attention from the press and society. Veterinary pathologists and civic authorities have a duty not only to elucidate the cause, method and manner of death but also to address the motivation behind a case. Poultry are commonly used as offering to gods in Santería rituals (ebós). Only a few cases have been reported in scientific journals, in contrast with the number of cases described in the media. Here, a hen and a cockerel (Gallus domesticus) were submitted to the VISAVET Health Surveillance Centre for autopsy, which comprised macroscopic and histological studies and microbiological testing for Chlamydia psittaci, avian orthoavulavirus 1 (Newcastle disease virus), West Nile virus and avian influenza virus. The carcasses were in an active decay stage of decomposition, containing larvae of Lucilia eximia. Both animals had been decapitated and haemorrhages in the cutting region were observed histologically. Post-mortem burns were also observed. To the best of the authors’ knowledge, this is the first post-mortem description of lesions in a domestic avian species due to religious rituals. European Union and Spanish forensic practitioners should be aware that these ritual sacrifices occur in some European countries. Moreover, future European and national legislation updates should consider this type of animal abuse.Item Immunopathology of early and advanced epididymis lesions caused by Brucella ovis in rams(Veterinary Immunology and Immunopathology, 2023) Rebollada Merino, Agustín Miguel; García-Seco Romero, María Teresa; Chinchilla Rodríguez, Blanca; Pérez Sancho, Marta; Domínguez Rodríguez, Lucas José; Rodríguez Bertos, Antonio ManuelOvine brucellosis is an infectious disease that causes alterations in the reproductive tract in ram and abortion in ewes. Their negative economic impact in ovine production warrants a thorough understanding the interactions between B. ovis and the host. Here, epididymis lesions of rams infected by B. ovis were histopathologically staged into early and advanced. Expression by immunohistochemistry of Brucella antigens, inflammatory cell markers (CD3, CD79αcy) and cytokines (IFN-γ, TNF-α, TGF-β1) was assessed in both stages. Early lesions were characterized by epithelial changes, interstitial inflammation, and mild fibrosis; whereas advanced lesions displayed caseous granulomas containing numerous macrophages, multinucleated giant cells, lymphocytes, and plasma cells. Expression of Brucella antigens were observed in both stages. The cellular response in B. ovis lesions were predominantly of T-cells (CD3+) whereas low numbers of B-cells and plasma cells (CD79αcy+) were present in both early and advanced lesions. IFN-γ was expressed by lymphocytes in early lesions suggesting that the adaptive immune response against B. ovis is initiated by Th1 cells, this response was also preserved in advanced stages. Expression of TNF-α was observed in neutrophils of epithelial microabscesses and intraepithelial T-cells of early lesions suggesting a promotion of neutrophil phagocytosis triggered by TNF-α. On the other hand, advanced lesions showed a reduction of TNF-α expression which may permit B. ovis persistence in granulomas. Lastly, TGF-β1 expression (fibroblast, macrophages and less in lymphocytes) were increased with time, suggesting that B. ovis promotes TGF-β1 secretion promoting chronicity of the lesions.- Project number: 163
Item Transparencia en la calidad docente: reflexión solidaria intradepartamental de las evaluaciones obtenidas en las encuestas del estudiantado del programa docentia. Análisis de los factores de mejora(2023) Arroyo Pardo, Eduardo; Álvarez Melcón, Ángela Concepción; Calero García, María Luz; Belando Montoro, María Remedios; Delgado Losada, María Luisa; González Matilla, Juan Francisco; Hernández Martín, Marta María; López Parra, Ana María; Aguilera Morales, Encarnación; Díaz Ramiro, Eva María; Fuentes Gómez-Calcerrada, Juan Luis; García Molto, Amelia Isabel Martina; González Martín, María Rosario; Hernández Ibáñez, María Del Carmen; Lopes Gomes, Claudia Filipa; López Matayoshi, César Yoshi; López Sánchez, Ramón; Moreno Pimentel, Antonio Gabriel; Palomo Díez, Sara; Prados Atienza, José María; Rebollada Merino, Agustín Miguel; Redondo Corcobado, Paloma; Rodríguez Bertos, Antonio Manuel; Royo Moreno, Fernando María; Rubio Valdehita, Susana; Tabanera De Lucio, Enrique; Martín Freire, Vanesa; Chinchilla Rodríguez, Blanca; Delgado Ramos Lima, Alice Helena; Porras González, NéstorEl objeto del proyecto es realizar una reflexión conjunta de profesores en diferentes departamentos, con el fin de identificar aquellos ítems, planteados por Docentia-UCM, que han arrojado resultados mejorables. Posteriormente serán contrastados con un panel de docentes calificados como “excelentes” (de los que algunos ya forman parte del presente proyecto), en base al programa, con excelencia en una jornada de reflexión, abierta a todos los profesores de cada departamento implicado, y a los delegados de las asignaturas impartidas por dicho departamento. Se busca la mejora de las evaluaciones de los profesores.