Person: Sánchez Rodríguez, Ana
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First Name
Ana
Last Name
Sánchez Rodríguez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Fisiología
Area
Fisiología
Identifiers
4 results
Search Results
Now showing 1 - 4 of 4
Item Caracterización y producción de β-NGF y su papel en la fisiología reproductiva del conejo(2019) Sánchez Rodríguez, Ana; García García, Rosa María; Lorenzo González, Pedro LuisEl factor de crecimiento nervioso (β-NGF) es una neurotrofina que se descubrió en el sistema nervioso y, posteriormente, en numerosas partes del organismo, entre ellos el sistema reproductor de muchas especies animales. Presenta importantes funciones durante el desarrollo de los órganos reproductores, como la espermatogénesis, la foliculogénesis, la maduración de los gametos y la ovulación. De hecho, se ha demostrado que es el denominado factor inductor de la ovulación (OIF) en especies de ovulación inducida, como los camélidos. En conejos, la presencia del sistema β-NGF (neurotrofina y sus dos receptores) se ha confirmado en algunos órganos masculinos (testículos, próstata y glándulas seminales) y femeninos (ovarios, oviducto, útero y cérvix) de animales adultos, pero no se ha caracterizado su papel durante el desarrollo sexual ni se conoce su relación con otras hormonas importantes en la reproducción, como la testosterona. Aunque el conejo es una especie de ovulación inducida, hasta el momento no se ha demostrado si el β-NGF provoca la ovulación como en camélidos puesto que los estudios realizados no han obtenido una respuesta ovulatoria adecuada con plasma seminal (PS) homólogo ni heterólogo, ni con β-NGF de origen murino...Item Recombinant rabbit beta nerve growth factor production and its biological effects on sperm and ovulation in rabbits(PLoSONE, 2019) Sánchez Rodríguez, Ana; Abad, Paloma; Arias Álvarez, María; Millán Pastor, María Pilar; García Rebollar, Pilar; Bautista Santa Cruz, José Manuel; Lorenzo González, Pedro Luis; García García, Rosa MaríaIn some induced-ovulating species, beta nerve growth factor (β-NGF) has important roles in ovulation, though data for rabbits are still inconclusive. In this study we first synthesized functional recombinant β-NGF from rabbit tissue (rrβ-NGF) to address the following objectives: 1) to compare rabbit β-NGF amino acid sequence with those of other induced- or spontaneous-ovulating species; 2) to assess the effects of rrβ-NGF on rabbit sperm viability and motility, and 3) to examine the in vivo ovulation inducing effect of rrβ-NGF added to the seminal dose in rabbit does. The NGF gene in rabbit prostate tissue was sequenced by Rapid Amplification of cDNA Ends and annotated in GenBank (KX528686). Recombinant rβ-NGF was produced in CHO cells and purified by affinity chromatography. Once confirmed by Western blotting and mass spectrometry (MALDI-TOF) that the amino acid sequence of the recombinant protein corresponded to β-NGF, its functionality was validated in PC12 cells in a successful dose-response study over 8 days. The amino acid sequence of prostate rabbit NGF differed to that of other species mainly in its receptor binding sites. In all the spontaneous ovulating species examined, compared with rabbit, alanine and proline residues, which interact with the high-affinity receptor, were replaced by a serine. In rabbits, asparagine and methionine were substituted by lysine at the low-affinity receptor binding site. In time- and dose-response experiments, the in vitro addition of rrβ-NGF to the ejaculate did not affect sperm viability whereas sperm motility parameters were enhanced by the addition of 1 μg/mL of the neuropeptide. Addition of this same concentration of rrβ-NGF to the seminal dose administered via the intravaginal route in does induced ovulation with a delayed LH peak, leading to a plasma progesterone increase, gestation and delivery. Our findings suggest that rrβ-NGF could be a useful option for biotechnological and reproduction assisted techniques in rabbits but further studies are needed.- Project number: 167
Item Digitalización de documentos para generar recursos educativos virtuales(2017) Revuelta Rueda, Luis; García García, Rosa María; Arias Álvarez, María; González Gil, Alfredo; Sanz Luengo, María del Mar; Sánchez Rodríguez, AnaLas nuevas tecnologías y los medios digitales nos ofrecen nuevas posibilidades educativas: e-learning, entorno BYOD (Bring Your Own Device), etc. Los cursos abiertos (MOOCs) y los entornos de formación virtual (VLE) permiten el intercambio de contenido e información a través de diversos canales. Pero estos medios, por sí solos, no garantizan conseguir resultados de aprendizaje si no se cuentan con contenidos educativos adecuados. Estos modelos educativos no deben contar sólo con recursos “ex profeso” sino que deben apoyarse en materiales existentes de calidad, como libros de texto, artículos de revistas especializadas y fondos gráficos de los museos de la facultad. Sin embargo estos recursos no se encuentran en formato digital en nuestras bibliotecas aunque son muy demandados. La imposibilidad de hacer bases de datos de los documentos escaneados en la biblioteca, obliga al escaneo continuo de los ejemplares y por lo tanto sufren una manipulación por reutilización que puede afectar a su conservación. Así pues, la digitalización por escáner “cenital” es una solución útil para obtener contenidos digitales de documentos delicados o antiguos. Item Characterization of β-Nerve Growth Factor-TrkA system in male reproductive tract of rabbit and the relationship between β-NGF and testosterone levels with seminal quality during sexual maturation(Theriogenology, 2019) Sánchez Rodríguez, Ana; Arias Álvarez, María; Timón, Patricia; Bautista Santa Cruz, José Manuel; García Rebollar, Pilar; Lorenzo González, Pedro Luis; García García, Rosa Maríaβ-Nerve Growth Factor (β-NGF) is a neurotrophin which acts through its receptors TrkA and p75, performing important actions in male reproductive physiology and its presence in seminal plasma (SP) has been related to male fertility. The aim of the present study was to evaluate the gene expression profile and the immunolocalization of β-NGF and its high-affinity receptor TrkA in sex organs in rabbits during sexual maturation period. β-NGF concentration for both SP and blood plasma (BP) and BP testosterone levels were determined as well as the seminal parameters during such period. Ten New Zealand White x California young rabbits were trained to semen collection since 20 weeks of age and routinely done once a week with two ejaculations per session. At 22 and 37 weeks of age, semen collection was carried out three times a week and seminal parameters were evaluated. Four males were randomly assigned and slaughtered in each age (n = 8); sex organs (prostate, bulbourethral glands and epididymis) were dissected and collected to determine β-NGF and TrkA gene expression and immunolocalization. SP and BP were also taken at each semen collection session to evaluate β-NGF concentration, and testosterone levels were also assessed in BP. The highest β-NGF mRNA expression was observed in prostate compared to bulbourethral glands and epididymis. These two last tissues showed residual β-NGF mRNA expression and limited localization of the neurotrophin. The prostate epithelial cells and lumen were strongly stained with regard to the other sex organs indicating that immunolocalization of β-NGF rely mainly in the prostate. TrkA gene expression was lower but constant and differentially immunolocalized in the sex organ tissues. Finally, β-NGF concentration in SP and BP remained unchanged in accordance to age, while some seminal characteristics such as sperm concentration, percentage of live sperm and mass and progressive motility were enhanced as endowed by BP testosterone variation. β-NGF and its cognate TrkA receptor are expressed and immunolocalized in the male reproductive tract in the two ages studied, independently of the circulating levels of testosterone and β-NGF.