Person:
Mas Zubiri, Alicia

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First Name
Alicia
Last Name
Mas Zubiri
URI
https://hdl.handle.net/20.500.14352/84522
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
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End date: 2019 × Subject: 535 ×

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    Label-free bioanalysis of Leishmania infantum using refractive index tomography with partially coherent illumination
    (Journal of biophotonics, 2019) Soto Rueda, Juan Manuel; Rodrigo Martín-Romo, José Augusto; Mas Zubiri, Alicia; Domínguez Bernal, Gustavo Ramón; Alieva Krasheninnikova, Tatiana
    In this work, we report the use of refractive index (RI) tomography for quantitative analysis of unstained DH82 cell line infected with Leishmania infantum. The cell RI is reconstructed by using a modality of optical diffraction tomography technique that employs partially coherent illumination, thus enabling inherent compatibility with conventional wide-field microscopes. The experimental results demonstrate that the cell dry mass concentration (DMC) obtained from the RI allows for reliable detection and quantitative characterization of the infection and its temporal evolution. The RI provides important insight for studying morphological changes, particularly membrane blebbing linked to an apoptosis (cell death) process induced by the disease. Moreover, the results evidence that infected DH82 cells exhibit a higher DMC than healthy samples. These findings open up promising perspectives for clinical diagnosis of Leishmania.