Person: San Andrés Larrea, Manuel Ignacio
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First Name
Manuel Ignacio
Last Name
San Andrés Larrea
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Farmacología y Toxicología
Area
Farmacología
Identifiers
5 results
Search Results
Now showing 1 - 5 of 5
Item PK/PD Analysis by Nonlinear Mixed-Effects Modeling of a Marbofloxacin Dose Regimen for Treatment of Goat Mastitis Produced by Coagulase-Negative Staphylococci(Animals, 2021) Lorenzutti, Augusto Matías; Vico, Juan Pablo; Serrano-Rodríguez, Juan Manuel; Himelfarb, Martín Alejandro; San Andrés Larrea, Manuel Ignacio; Lucas Burneo, José Julio De; Litterio, Nicolás JavierCoagulase-negative staphylococci are main pathogens that produce goat mastitis. Marbofloxacin is a third-generation fluoroquinolone approved for treat mastitis in animals. The objectives of this study were: (i) to determine the pharmacokinetics of marbofloxacin (10 mg/kg/24 h) in serum and milk administered intramuscularly for five days in goats with mastitis induced by coagulase-negative staphylococci; (ii) to characterize the concentration–effect relationship of marbofloxacin against coagulase-negative staphylococci in Mueller Hinton broth and goat milk; (iii) to determine AUC/MIC cutoff values of marbofloxacin, and (iv) to perform a PK/PD analysis to evaluate the efficacy of the dose regimen for the treatment of goat mastitis produced by coagulase-negative staphylococci. Marbofloxacin presented context-sensitive pharmacokinetics, influenced by the evolution of the disease, which decreased marbofloxacin disposition in serum and milk. Marbofloxacin showed a median (95% CI) fAUC/MIC values for MIC of 0.4 and 0.8 µg/mL of 26.66 (22.26–36.64) and 32.28 (26.57–48.35) related with −2 log10CFU/mL reduction; and 32.26 (24.81–81.50) and 41.39 (29.38–128.01) for −3 log10CFU/mL reduction in Mueller Hinton broth. For milk, −2 log10CFU/mL reduction was achieved with 41.48 (35.29–58.73) and 51.91 (39.09–131.63), and −3 log10CFU/mL reduction with 51.04 (41.6–82.1) and 65.65 (46.68–210.16). The proposed dose regimen was adequate for the treatment of goat mastitis produced by coagulase-negative staphylococci, resulting in microbiological and clinical cure of all animals. The animal model used in this study provided important pharmacokinetic information about the effect of the infection on the pharmacokinetics of marbofloxacin. Pharmacodynamic modeling showed that fAUC/MIC cutoff values were higher in goat milk compared with Mueller Hinton broth.- Project number: 64
Item Hygia pecoris, salus populi ¿Atención y compromiso se reflejan en bienestar emocional?(2021) Lucas Burneo, Yolanda de; Andrés Gamazo, Paloma Jimena De; Rodríguez Fernández, Casilda; Lorenzutti, Augusto Matías; Muñoz Serrano, María Carmen; García Fernández, Rosa Ana; Cummings, Michael Ryan; Cassarrubios Calvo, Diana; Jiménez Rubio, Laura; Litterio, Nicolás Javier; Zarazaga, María del Pilar; Tejero Martín, Coral; San Andrés Larrea, Manuel Ignacio; Lucas Burneo, José Julio DeDesde un enfoque “one health”, la salud de los animales se refleja en la salud pública y la salud medioambiental. ¿La buena praxis de los profesionales responsables de la salud animal, se refleja en el bienestar emocional de estos profesionales sanitarios?. La OMS define el bienestar emocional como ese estado de ánimo en el cual la persona se da cuenta de sus propias aptitudes, puede afrontar las presiones normales de la vida, trabajar productivamente y contribuir a la comunidad. El estudio WSAVA 2019, sobre bienestar veterinario, ansiedad y depresión, muestra que la salud mental de los profesionales más jóvenes está seriamente en riesgo. EL estrés laboral ha sido catalogado por la OMS como la epidemia del siglo XXI. El desarrollo del programa atención consciente y gestión emocional, en el marco académico en el que se integra, se alinea con los objetivos de desarrollo sostenible de la ONU (Agenda 2030) ODS3 y transversalmente con ODS5 y ODS16. Item Effect of chemically modified tetracycline-8 (CMT-8) on hematology, blood chemistry, cytokines and peripheral blood lymphocyte subsets of healthy dogs(Research in Veterinary Science, 2021) Sainz Rodríguez, Ángel; García-Sancho Téllez, Mercedes Guadalupe; Rodríguez Franco, Fernando; San Andrés Larrea, Manuel Ignacio; Rodríguez Fernández, Casilda; Lucas Burneo, José Julio De; San Andrés Larrea, María Dolores De; Agulla Pérez, Beatriz; Villaescusa Fernández, AlejandraTetracyclines are antibiotics widely used in human and veterinary medicine. Effects on the immune system and inflammatory response, including effects on blood leukocytes proliferation and function and in cytokines synthesis, have been described. Chemically modified tetracyclines (CMT) have lost their antimicrobial activity, but maintain these other properties. This study analyzes the effect of chemically modified tetracycline-8 (CMT-8) on the evolution of complete blood count, blood chemistry, the mRNA expression of selected cytokines and peripheral blood lymphocyte subpopulations distribution in healthy dogs. CMT-8 at a dose of 10 mg/kg once daily was administered per os to six healthy dogs. A control group of five healthy dogs, living in the same conditions than dogs treated with CMT-8, received placebo with an identical therapeutic regimen. When given at the doses used in this study, no side effects of CMT-8 were detected, suggesting a good tolerance and a limited toxicity of the drug. Dogs treated with CMT-8 showed a gradual increase in mean corpuscular hemoglobin. The administration of CMT-8 in healthy dogs did not affect blood mRNA expression of IFN-γ, TNFα, IL-4, IL-6, IL-10, IL-12 p40 and IL-13. However, the lymphocytes expressing class II MHC on their surface decreased during the first two weeks of CMT-8 treatment and subsequently increased for the next three months. Considering the absence of antimicrobial properties of the drug, the effects of CMT-8 detected in this study seem to be unrelated to the classical antimicrobial activity attributed to tetracyclines.Item Pharmacokinetic Behaviour of Enrofloxacin after Single Intramuscular Dosage in American Black Vultures (Coragyps atratus)(Antibiotics, 2021) Waxman, Samanta; Lucas Burneo, José Julio De; Wiemeyer, Guillermo; Torres Bianchini, Laura; San Andrés Larrea, Manuel Ignacio; Rodríguez Fernández, CasildaThe aim of the study was to investigate the intramuscular pharmacokinetics of enrofloxacin in black vultures (Coragyps atratus). The pharmacokinetics of a single intramuscular dose (10 mg/kg) of enrofloxacin was studied in six vultures. Plasma concentrations of enrofloxacin and its active metabolite, ciprofloxacin, were determined by high-performance liquid chromatography (HPLCuv). Pharmacokinetic parameters were estimated using non-compartmental and compartmental analysis. After intramuscular administration, enrofloxacin showed a rapid and complete absorption, reaching a Cmax value of 3.26 ± 0.23 μg/mL at 1.75 ± 0.53 h. A long terminal half-life of 19.58 h has been observed. Using previously published MIC values to perform a PK/PD analysis, cumulative fraction responses obtained after Monte Carlo simulation for AUC/MIC > 30, 50 and 125 were 72.93%, 72.34% and 30.86% for E. coli and 89.29%, 88.89% and 58.57% for Mycoplasma synoviae, respectively. Cumulative fraction responses obtained for Cmax/MIC index were 33.93% and 40.18% for E. coli and M. synoviae, respectively. The intramuscular administration of 10 mg/kg could be appropriate to treat infectious diseases caused by gram-positive bacteria with MIC value lower than 1 µg/mL; however, although enrofloxacin showed a slow elimination in black vultures, plasma concentrations were insufficient to reach the gram-negative stablished breakpoints.- Project number: 100
Item Conviviendo en Positivo: Educación en salud, Atención Consciente, Compasión activa y Resiliencia(2020) Lucas Burneo, José Julio De; Lucas Burneo, Yolanda de; Casarrubios Calvo, Diana; Gutiérrez Sánchez, Patricia; Jiménez Rubio, Laura; López García, Belén; Matías Lorenzutti, Augusto; Maestro García, Sara; Muñoz Serrano, María Carmen; Panizo Benza, Irene; Rodríguez Fernández, Casilda; Rodríguez Martínez, María; Ruíz-Roso Martín, Laura María; San Andrés Larrea, Manuel Ignacio; Sánchez Prada, Raquel; Sierra Díaz, Ana María; Siso Callejo, Laura; Tejero Martín, CoralProyecto de innovación docente enfocado en valores nucleares y convivencia en una sociedad serodiscordante, es continuidad del P89/2018, titulado “competencias intra e interpersonales, sexo consciente, fármacos y mindfulness”. Durante el primer semestre, desde el paradigma de aprendizaje cooperativo, basado en resolución de proyectos, se desarrolló la investigación documental, reflexión y debate sobre estos centros de interés: valores nucleares & VIH: abordaje de la seropositividad; adherencia terapéutica; estudio PARTNER indetectable es igual a intransmisible; profilaxis pre-exposición (PrEP); convivir en serodiscordancia; encuestas sobre estigmatización y discriminación social y sanitaria; riesgos globales de la discriminación terapéutica; relaciones interpersonales y emociones aflictivas; mindfulness y compasión basado en la evidencia; psicología positiva y resiliencia. En el segundo semestre, durante la fase cero del estado de alerta sanitaria, se desarrolló una intervención psicoeducativa, dirigida a facilitar estrategias que ayudasen a gestionar mejor el impacto emocional generado por la incertidumbre, la restricción de movilidad territorial, el confinamiento, y el miedo al contagio de la COVID-19. Perpetuar el estigma ante VIH, es una violación de los derechos humanos. Como antídoto a la indiferencia, se integró en nuestra intervención el fomento de la compasión activa, entendida como un sentido básico de cuidado, sensibilidad y apertura hacia el sufrimiento propio y de los demás, y la intención genuina de intentar aliviarlo y prevenirlo. La pandemia generada por el SARS-CoV-2 es una oportunidad para entender que ante este virus, aquí y ahora, la única opción es prevenirlo, detectarlo mediante pruebas y confinarlo, es decir no transmitirlo y no reinfectarse. Si de forma transversal somos capaces de interiorizarlo y normalizar la convivencia en serodiscordancia, si normalizamos las pruebas diagnósticas rutinarias, podremos entre todas y todos reducir hasta un 90% la transmisión activa otra pandemia de la que hoy no se habla, del VIH, de este modo conseguiríamos minimizar la aparición de nuevos casos, frenar su avance y vencer definitivamente al SIDA.