Person: Gómez Graña, Sergio
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First Name
Sergio
Last Name
Gómez Graña
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
Identifiers
4 results
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Item Targeted Chemo‐Photothermal Therapy: A Nanomedicine Approximation to Selective Melanoma Treatment(2018) Villaverde Cantizano, Gonzalo; Gómez Graña, Sergio; Guisasola, Eduardo ; García García, María Isabel; Hanske, Christoph ; Liz‐Marzán, Luis M. ; Baeza, Alejandro ; Vallet Regí, María Dulce NombreMelanoma is one of the most severe public health issues worldwide, not only because of the high number of cases but also for its poor prognosis in late stages. Therefore, early diagnosis and efficient treatment are key toward a future solution. However, melanoma is highly resistant to cytotoxicity in its metastatic form. In this context, a therapeutic strategy based on a targeted chemo-photothermal nanotransporter for cytotoxic compounds is proposed. This approach comprises the use of core–multishell gold nanorods, coated with mesoporous silica and further covered with a thermosensitive polymer, which is vectorized for selective internalization in melanoma cells. The proposed nanoformulation is capable of releasing the transported cytotoxic compounds on demand, in response to near-IR irradiation, with high selectivity and efficacy against malignant cells, even at low concentrations, thereby providing a new tool against melanoma disease.Item Nanoparticles for Multimodal Antivascular Therapeutics: Dual Drug Release, Photothermal and Photodynamic Therapy.(Acta Biomaterialia, 2019) Paris, J. L.; Villaverde Cantizano, Gonzalo; Gómez Graña, Sergio; Vallet Regí, María Dulce NombreThe poor delivery of nanoparticles to target cancer cells hinders their success in the clinical setting. In this work, an alternative target readily available for circulating nanoparticles has been selected to eliminate the need for nanoparticle penetration in the tissue: the tumor blood vessels. A tumor endothelium-targeted nanoparticle (employing an RGD-containing peptide) capable of co-delivering two anti-vascular drugs (one anti-angiogenic drug and one vascular disruption agent) is here presented. Furthermore, the nanodevice presents two additional anti-vascular capabilities upon activation by Near-Infrared light: provoking local hyperthermia (by gold nanorods in the system) and generating toxic reactive oxygen species (by the presence of a photosensitizer). RGD-targeting is shown to increase uptake by HUVEC cells, and while the nanoparticles are shown not to be toxic for these cells, upon Near-Infrared irradiation their almost complete killing is achieved. The combination of all four therapeutic modalities is then evaluated in an ex ovo fibrosarcoma xenograft model, which shows a significant reduction in the number of blood vessels irrigating the xenografts when the nanoparticles are present, as well as the destruction of the existing blood vessels upon irradiation. These results suggest that the combination of different anti-vascular therapeutic strategies in a single nanocarrier appears promising and should be further explored in the future.Item Nanoparticles for multimodal antivascular therapeutics: Dual drug release, photothermal and photodynamic therapy(2020) Paris, Juan L.; Villaverde Cantizano, Gonzalo; Gómez Graña, Sergio; Vallet Regí, María Dulce NombreThe poor delivery of nanoparticles to target cancer cells hinders their success in the clinical setting. In this work, an alternative target readily available for circulating nanoparticles has been selected to eliminate the need for nanoparticle penetration in the tissue: the tumor blood vessels. A tumor endotheliumtargeted nanoparticle (employing an RGD-containing peptide) capable of co-delivering two anti-vascular drugs (one anti-angiogenic drug and one vascular disruption agent) is here presented. Furthermore, the nanodevice presents two additional anti-vascular capabilities upon activation by Near-Infrared light: provoking local hyperthermia (by gold nanorods in the system) and generating toxic reactive oxygen species (by the presence of a photosensitizer). RGD-targeting is shown to increase uptake by HUVEC cells, and while the nanoparticles are shown not to be toxic for these cells, upon Near-Infrared irradiation their almost complete killing is achieved. The combination of all four therapeutic modalities is then evaluated in an ex ovo fibrosarcoma xenograft model, which shows a significant reduction in the number of blood vessels irrigating the xenografts when the nanoparticles are present, as well as the destruction of the existing blood vessels upon irradiation. These results suggest that the combination of different antivascular therapeutic strategies in a single nanocarrier appears promising and should be further explored in the future.Item Targeted Chemo‐Photothermal Therapy: A Nanomedicine Approximation to Selective Melanoma Treatment(Particle & Particle Systems Characterization, 2018) Villaverde Cantizano, Gonzalo; Gómez Graña, Sergio; Guisasola, Eduardo; García García, María Isabel; Hanske, Cristoph; Liz-Marzan, Luis M.; Baeza, Alejandro; Vallet Regí, María Dulce NombreMelanoma is one of the most severe public health issues worldwide, not onlybecause of the high number of cases but also for its poor prognosis in late stages. Therefore, early diagnosis and efficient treatment are key toward a future solution. However, melanoma is highly resistant to cytotoxicity in its metastatic form. In this context, we propose a therapeutic strategy based on a targeted chemo-photothermal nanotransporter for cytotoxic compounds. This approach comprises the use of core-multishell gold nanorods, coated with mesoporous silica and further covered with a thermosensitive polymer, which is vectorized for selective internalization in melanoma cells. The proposed nanoformulation is capable of releasing the transported cytotoxic compounds on demand, in response to near-IR irradiation, with high selectivity and efficacy against malignant cells, even at low concentrations, thereby providing a new tool against melanoma disease.