Person: Beroiz Remírez, Beatriz
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First Name
Beatriz
Last Name
Beroiz Remírez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Genética, Fisiología y Microbiología
Area
Genética
Identifiers
5 results
Search Results
Now showing 1 - 5 of 5
Item Caracterización morfométrica y genética de las poblaciones españolas de cangrejo de río "Austropotamobius pallipes" (Lereboullet, 1858) mediante el uso de marcadores moleculares(2005) Beroiz Remírez, Beatriz; Ochando, Maria Dolores; Callejas Hervás, Ma. del CarmenEl cangrejo de río Austropotamobius pallipes (Lereboullet, 1858) está incluido en la Lista Roja de la U.I.C.N. (Unión Internacional para la Conservación de la Naturaleza) en la categoría de "vulnerable", al igual que en el Catálogo Nacional de especies amenazadas por su tendencia poblacional regresiva que le ha llevado a desaparecer de una gran parte de su área de distribución anterior (BOE nº 149 de 23 de junio de 2003).Dada la situación que atraviesa el cangrejo de río nos hemos planteado, mediante la utilización de la metodología RAPD-PCR y marcadores morfométricos, el estudio de sus poblaciones. La combinación simultánea de ambos marcadores permite intentar conocer a fondo la estructura poblacional de la especie A. pallipes en España. Los patrones de distribución de la variabilidad, tanto genética como morfométrica observada en estas poblaciones, evidencian cierto grado de diferenciación entre ellas, probablemente fruto de la antigüedad del establecimiento y de la fragmentación que las poblaciones han sufrido. No existe una relación genético/morfométrico-geográfica ni genético/morfométrico-hidrográfica clara. Puede estar en relación con los movimientos de cangrejos de unos lugares a otros efectuados por el hombre con el fin de perpetuar las poblaciones de cangrejo, o con las fragmentaciones, que han propiciado pequeños tamaños efectivos de las poblaciones y endogamia, posiblemente como consecuencia de los múltiples cuellos de botella donde la deriva genética ha podido jugar un papel muy importante. Estos cuellos de botella que han atravesado las poblaciones podrían deberse principalmente a las diferentes causas que han conducido a la regresión del cangrejo en nuestro país, entre las que se encuentran las alteraciones producidas por el hombre sobre el hábitat, la afanomicosis y la introducción de especies exóticas de cangrejos.La información obtenida puede resultar eficaz para las Administraciones a la hora de diseñar programas de recuperación de las poblaciones de cangrejo autóctono.Item Genetic structure of Sesamia nonagrioides (Lefebvre) populations in the mediterranean area(Environmental Entomology, 2008) Beroiz Remírez, Beatriz; Poza, Marta De la; Pérez Farinós, Gema; Ortego, FélixThe Mediterranean corn borer, Sesamia nonagrioides (Lefebvre) (Lepidoptera: Noctuidae), is a major pest of maize in the Mediterranean area. Transgenic Bt maize expressing the Cry1Ab toxin from the bacterium Bacillus thuringiensis can effectively control this pest. The characterization of S. nonagrioides population structure, at a large geographical scale, would provide some insight in decision making for resistance management. The genetic relationships among nine populations from Spain, one from France, one from Italy, three from Greece, and one from Turkey were assessed using Random Amplyfied Polymorphic DNA (RAPD) markers. Populations from France and Spain formed a cluster independent from a cluster of populations collected in Italy, Turkey, and Greece in a unweighted pair-group method with arithmetic average dendrogram constructed from Nei’s genetic distances. Average genetic differentiation among samples was significant for all geographical groupings analyzed (FST = 0.160 ± 0.014 for Spanish populations; 0.133 ± 0.022 for Spanish and French populations; and 0.095 ± 0.010 for Greek, Italian, and Turkish populations). Genetic differentiation was also significant for all paired comparisons of populations, including two Spanish populations separated by only 15 km with no apparent geographical barriers. No pattern of isolation by distance was observed among Mediterranean corn borer populations collected in Spain and France. These results suggest a limited genetic exchange between relatively distant S. nonagrioides populations in Europe, which might contribute to decreased rate of spread of resistance alleles once resistance has developed at a certain site.Item Genetic structure of Spanish white-clawed crayfish (Austropotamobius pallipes) populations as determined by RAPD analysis: reasons for optimism(Aquatic conservation: marine and freshwater ecosystems, 2008) Beroiz Remírez, Beatriz; Callejas Hervás, Carmen; Alonso, Fernando; Ochando González, María Dolores1. Spanish populations of the white-clawed crayfish have declined sharply over the last three decades. Although Austropotamobius pallipes was once widely distributed and very abundant in most of the limestone basins of the country, outbreaks of crayfish plague since 1978 have reduced its populations, and now only some 500–600 small populations are left. 2. Consequently, the species now enjoys protection under national legislation. Management decisions regarding the conservation of a threatened species require an understanding of the genetic structure of its populations. 3. Using random amplified polymorphic DNA (RAPD) fingerprinting the genetic variability of 11 populations of A. pallipes was assessed over the species' range in Spain, and their phylogenetic relationships determined. 4. Substantial genetic differentiation was detected among the populations tested; no clear relationship was found between patterns of genetic variability and hydrological basin. The RAPD markers showed the degree of genetic variability of these populations to be similar to, and in some cases slightly higher than, that reported in previous studies on other Spanish and European populations of A. pallipes. 5. The results offer hope for the recovery of this species in Spain, and provide information that might be useful in the management of crayfish reintroduction programmes.Item Population structure of Banana Weevil, an introduced pest in the Canary Islands, studied by RAPD analysis(Bulletin of Entomological Research, 2007) Magaña, Cristina; Beroiz Remírez, Beatriz; Hernández Crespo, Pedro; Montes de Oca, M.; Carnero, A.; Ortego, Félix; Castañera, PedroThe banana weevil (BW), Cosmopolites sordidus (Coleoptera: Curculionidae), is one of the most important insect pests of bananas and plantains. The mobility and the origin of BW infestations at the Canary Islands (Tenerife, La Gomera and La Palma) have been analysed using Random Amplified Polymorphic DNA (RAPD) as molecular markers. Populations from Costa Rica, Colombia, Uganda and Madeira were also included for comparison. One hundred and fifteen reproducible bands from eight primers were obtained. The level of polymorphism in the populations from the Canary Islands (40–62%) was in the range of those found in other populations. Nei's genetic distances, pair-wise fixation index (FST) values indicate that the closest populations are Tenerife populations among themselves (Nei's genetic distance=0.054–0.100; FST=0.091–0.157) and Costa Rica and Colombia populations (Nei's genetic distance=0.049; FST=0.113). Our results indicate the existence of BW local biotypes with limited gene flow and affected by genetic drift. These results are compatible with a unique event of colonization at Tenerife; whereas, the outbreaks in La Gomera and La Palma may come from independent introductions. The Madeira population is phylogenetically and geographically closer to the Canary Islands populations, suggesting that it is the most likely source of the insects introduced in the Canary Islands.Item Mapa 800: Populus tremula L.(Asientos para un atlas corológico de la flora occidental, 25, 2002) Andrés Castillo, Mª Luz; Beroiz Remírez, Beatriz; Montalvo Morales, Encarna; Fernández Casas, Francisco Javier; Fernández Sánchez, Abel JuanEsta vigésimoquinta nota contiene mapas nuevos y añade asientos en mapas previamente publicados.