Person: Zuluaga Arias, María Del Pilar
Loading...
First Name
María Del Pilar
Last Name
Zuluaga Arias
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Estadística e Investigación Operativa
Area
Estadística e Investigación Operativa
Identifiers
7 results
Search Results
Now showing 1 - 7 of 7
Item Immunocytochemical detection of synaptophysin in C57BL/6 mice cochlea during aging process(Brain research reviews, 2009) Bartolomé Pascual, María Visitación; Zuluaga Arias, María Del Pilar; Carricondo Orejana, Francisco Javier; Gil Loyzaga, PabloAged mammals frequently exhibit a bilateral, progressive, and symmetric deafness related to the degeneration of auditory receptor. However, little is still known about aging effects on synapses in this receptor. Synaptophysin (Syp) is a 38 kDa Ca2+ binding glycoprotein widely found in presynaptic membrane and vesicles. The Syp has been found in presynaptic buttons of efferent auditory fibers, within the developing and adult auditory receptor. The detection of Syp in aged cochleae could provide relevant information about synaptic changes and receptor degeneration process observed in old animals. This paper focuses on aging linked changes related to the presence of Syp in cochleae of C57BL/6J mice (from 1 to 24 months old). Results showed that during the first months of age, no significant changes were observed in the Syp distribution under the basal pole of inner (IHCs) neither the outer (OHCs) hair cells. At six months of age, a significant decrease of Syp immunocytochemical detection appeared in fibers under the most external row of OHCs, but restricted to the cochlear basal coil. Only a very scarce reduction of Syp was noted under the IHC and the other OHC rows, also at the basal coil. From mice 9 months old on, a progressive decrease of the presence of Syp was found under IHC and all OHC rows starting at the basal coil and reaching the apical coil in the oldest mice. All these data could indicate that the cochlea aging process early affects to presynaptic membrane proteins of efferent endings fibers. This early alteration of cochleae efferent synapses could be involved in the whole degeneration of the Corti's organ.Item Self-perception of the acquisition of transferable competencies by the participants in a research congress for undergraduate students: A cross-sectional study(Heliyon, 2024) Arráez Aybar, Luis Alfonso; Arias Díaz, Javier; Paredes Royano, Sergio Damián; Zuluaga Arias, María Del Pilar; Chevalier Del Río, Margarita; García Martín, Elena Salobrar; Collado Yurrita, Luis Rodolfo; Hurtado Moreno, Olivia; Fernández Mateos, María Del PilarContext: Several curricular initiatives have been developed to improve the acquisition of research competencies by Health Science students. Objectives: To know how students self-perceived of whether their participation in the XIV National Research Congress for Undergraduate Students of Health Sciences had helped them in the acquisition of 36 research-related transferable competencies (TCs) common to Health Science degrees. Methods: A survey design (Cronbach's alpha = 0.924), using a self-administered questionnaire, was conducted among undergraduate students who voluntarily participated in the Congress. Data analysis was performed using SPSS 25 and Statgraphics 19. Statistical significance was considered for P < 0.05. Results: Eighty-one students from 12 Health Science degree programs responded. Key findings are presented in a structured manner, using a Likert-5 scale. Twenty-five of the competencies surveyed obtained an average ≥ 4 highlighting: "Critically evaluate and know how to use sources of clinical and biomedical information to obtain, organize, interpret, and communicate scientific and health information"; "To be able to formulate hypotheses, collect and critically evaluate information for problem solving, following the scientific method", "Critical analysis and research" and "Communicate effectively and clearly, orally and in writing with other professionals". Significance was found in 15 competencies. The development of the competencies "Teamwork", "Critical reasoning" and "Analysis and synthesis abilities" was considered to be of greater "personal utility" by the respondents. Conclusion: Participation in this event contributed to the development of research-related TCs, critical analysis and information management and communication, especially in relation to learning the sources of clinical and biomedical information, to know, following the scientific method, how to formulate hypotheses that allow students to solve problems in their professional activity. The experience was significantly influenced by the respondents' year, the type of participation in the event and the gender of the students. Limitations and suggestions regarding future research are discussed to encourage further exploration of the topic.- Project number: 443
Item El formato vídeo como herramienta para el aprendizaje de la Histología. Implementación de “HistoApp” para la autoevaluación individual y asistida del alumno como refuerzo para el aprendizaje(2023) Buño Borde, Ismael; Sacedón Ayuso, Rosa; Novillo Villajos, Apolonia; Jiménez Pérez, Eva; Zuluaga Arias, María Del Pilar; Escribano Martínez, Catalina; Orera Clemente, María Asunción; Ortega Salmerón, Lucía; Buño Borde, Ismael Item Brain signal complexity in adults with Down syndrome: Potential application in the detection of mild cognitive impairment(Frontiers in Aging Neuroscience, 2022) Fernández Lucas, Alberto Amable; Ramírez Toraño, Federico; Bruña Fernández, Ricardo; Zuluaga Arias, María Del Pilar; Esteba Castillo, Susanna; Abásolo, Daniel; Moldenhauer, Fernando; Shumbayawonda, Elizabeth; Maestu Unturbe, Fernando; García Alba, JavierBackground: Down syndrome (DS) is considered the most frequent cause of early-onset Alzheimer’s disease (AD), and the typical pathophysiological signs are present in almost all individuals with DS by the age of 40. Despite of this evidence, the investigation on the pre-dementia stages in DS is scarce. In the present study we analyzed the complexity of brain oscillatory patterns and neuropsychological performance for the characterization of mild cognitive impairment (MCI) in DS. Materials and methods: Lempel-Ziv complexity (LZC) values from restingstatemagnetoencephalography recordings and the neuropsychological performance in 28 patients with DS [control DS group (CN-DS) (n = 14), MCI group (MCI-DS) (n = 14)] and 14 individuals with typical neurodevelopment (CN-no-DS) were analyzed. Results: Lempel-Ziv complexity was lowest in the frontal region within the MCI-DS group, while the CN-DS group showed reduced values in parietal areas when compared with the CN-no-DS group. Also, the CN-no-DS group exhibited the expected pattern of significant increase of LZC as a function of age, while MCI-DS cases showed a decrease. The combination of reduced LZC values and a divergent trajectory of complexity evolution with age, allowed the discrimination of CN-DS vs. MCI-DS patients with a 92.9% of sensitivity and 85.7% of specificity. Finally, a pattern of mnestic and praxic impairment was significantly associated in MCI-DS cases with the significant reduction of LZC values in frontal and parietal regions (p = 0.01). Conclusion: Brain signal complexity measured with LZC is reduced in DS and its development with age is also disrupted. The combination of both features might assist in the detection of MCI within this population.Item S-Adenosylmethionine Decreases Bacterial Translocation, Proinflammatory Cytokines, Oxidative Stress and Apoptosis Markers in Hepatic Ischemia-Reperfusion Injury in Wistar Rats(Antioxidants, 2023) Valdés, Sergio; Paredes Royano, Sergio Damián; García Carreras, Carmen; Zuluaga Arias, María Del Pilar; Rancán, Lisa; Linillos Pradillo, Beatriz; Arias Díaz, Javier; Vara Ameigeiras, Elena MaríaHepatic ischemia/reperfusion injury (IRI) can seriously impair liver function. It is initiated by oxidative stress, resulting in inflammation and apoptosis-induced cellular damage. Glutathione (GSH) prevents oxidative stress. S-Adenosylmethionine (SAMet) is a GSH synthesis precursor that avoids the deficit in SAMet-synthetase activity and contributes to intracellular ATP repletion. It also acts as a methyl group donor, stabilizing hepatocyte membranes, among other functions. This study investigated the effect of SAMet on bacterial translocation and levels of proinflammatory cytokines, oxidative stress and apoptosis markers in male Wistar rats subjected to hepatic IRI. Animals were randomly divided into six groups: (1) sham operation, (3) animals undergoing 60 min of ischemia of the right lateral lobe for temporary occlusion of the portal vein and hepatic artery plus 10 min of reperfusion, and (5) the same as (3) but with a reperfusion period of 120 min. Groups 2, 4 and 6, respectively, are the same as (1), (3) and (5), except that animals received SAMet (20 mg/kg) 15 min before ischemia. GSH, ATP, lipid peroxidation (LPO), TNF-α, IL-1β, IL-6, total caspase-1 and caspase-9, total and cleaved caspase-3, and phosphatidylcholine were determined in the liver. Endotoxin, TNF-α, IL-1β, IL-6, IL-10 and LPO in vena cava and portal vein blood samples were also measured. Endotoxin and LPO levels as well as proinflammatory cytokines and apoptotic markers increased significantly in animals undergoing IRI, both after 10 and 120 min of reperfusion. IRI produced a significant decrease in GSH, ATP, portal IL-10 and phosphatidylcholine. SAMet treatment prevented these effects significantly and increased survival rate. The study suggests that SAMet exerts protective effects in hepatic IRI.Item The Impact of Adult ADHD in the Quality of Life Profile(Journal of Attention Disorders, 2019) Quintero Gutiérrez Del Álamo, Francisco Javier; Morales, Isabel; Vera, Rosa; Zuluaga Arias, María Del Pilar; Fernández Lucas, Alberto AmableIn this multicenter, cross-sectional study, we aimed to investigate the quality of life (QoL) and the neuropsychological and clinical characteristics of adults with ADHD with different developmental pathways. Method: Our study sample included 25 control (healthy) participants, 31 patients with newly diagnosed ADHD without comorbidities (ADHD-C-D), 31 with newly diagnosed ADHD with comorbidities (ADHD+C-D), and 29 with previously diagnosed ADHD with comorbidities (ADHD+C+D). Results: Compared with controls, ADHDs had little differences in the attentional performance but showed higher impulsivity, more severe symptoms of depression and anxiety, and lower QoL. The ADHD+C-D group showed more severe symptoms of depression and anxiety than the ADHD+C+D group (p = .037 and p = .008, respectively), and poorer QoL in the psychological health sphere (p = .003). Conclusion: Differences between ADHD and control subjects were particularly remarkable in mood symptoms and QoL. Previous diagnosis might have a positive impact on mood symptoms and QoL in ADHD adultsItem Physical activity effects on the individual alpha peak frequency of older adults with and without genetic risk factors for Alzheimer’s Disease: A MEG study(Clinical Neurophysiology, 2018) De Frutos Lucas, Jaisalmer; López Sanz, David; Zuluaga Arias, María Del Pilar; Rodríguez Rojo, Inmaculada Concepción; Cazorla Luna, Raúl; López García, María Eugenia; Delgado Losada, María Luisa; Marcos Dolado, Alberto; Barabash Bustelo, Ana; López Sánchez, Ramón; Maestu Unturbe, Fernando; Fernández Lucas, Alberto AmableObjective Since a cure for Alzheimer’s Disease (AD) is yet to be discovered, attention has shifted towards prevention. Physical activity (PA) emerged as a notorious lifestyle factor that could influence brain structure and function. The individual alpha peak frequency (IAPF) is a measure that summarizes the spectral content of brain signals and has been proven to be sensitive to both AD pathology and PA interventions. Therefore, our goal was to unravel whether chronic PA modulates IAPF and if APOE ɛ4 carriage moderates this relationship. Methods We analyzed 4-minutes of resting-state magnetoencephalographic recordings from 100 healthy elders that provided self-reported measures of PA, and the IAPF was calculated. Results We found that IAPF was negatively influenced by age and APOE and positively influenced by PA. The effect of PA on IAPF only remained significant for the ɛ4 non-carriers group. Conclusions PA is positively associated to higher IAPF in healthy older adults and could potentially act as a protective factor against cognitive decline. Nevertheless, such effect is non-significant among elders who are more vulnerable to developing AD due to their genetic carriage. Significance This investigation offers the first neurophysiological evidences on the combined effects of APOE genotype and PA in healthy elders.