Person: Jiménez Meléndez, Alejandro
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First Name
Alejandro
Last Name
Jiménez Meléndez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
4 results
Search Results
Now showing 1 - 4 of 4
- Project number: 290
Item Parasitología Interactiva(2016) Gómez Bautista, Mercedes; Ortega Mora, Luis Miguel; Álvarez García, Gema; Ferré Pérez, Ignacio; Moreno Gonzalez, Javier; Gutierrez Expósito, Daniel; Arranz-Solís, David; Pastor Fernández, Iván; Rojo Montejo, Silvia; Sánchez Sánchez, Roberto; García Lunar, Paula; Jiménez Meléndez, Alejandro; Jimenez Pelayo, Laura; García Sánchez, Marta; Diezma Díaz, Carlos; Horcajo Iglesias, María Del Pilar; Regidor Cerrillo, Javier; Pérez Villalobos, Natividad; Collantes Fernández, EstherDesarrollo de una guía interactiva donde se muestran los ciclos biológicos,imágenes y dibujos de los estadios evolutivos de los principales géneros y especies de parásitos protozoos relevantes en al ámbito veterinario. - Project number: 262
Item Parasitología interactiva: Protozoos y afines(2018) Collantes Fernández, Esther; Gómez Bautista, Mercedes; Ortega Mora, Luis Miguel; Álvarez García, Gema; Ferré Pérez, Ignacio; García Lunar, Paula; Sánchez Sánchez, Roberto; Jiménez Meléndez, Alejandro; García Sánchez, Marta; Jiménez Pelayo, Laura; Diezma Díaz, Carlos; Regidor Cerrillo, Javier; Moreno González, Javier; Horcajo Iglesias, María Del PilarDesarrollo de una guía interactiva donde se muestran los ciclos biológicos, imágenes y dibujos de los estadios evolutivos de los principales géneros y especies de parásitos protozoos relevantes en al ámbito veterinario. Item Maternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates of Neospora caninum at Mid-Gestation(Frontiers in Cellular and Infection Microbiology, 2021) García-Sánchez, Marta; Jiménez-Pelayo, Laura; Regidor-Cerrillo, Javier; Osoro, Koldo; Vázquez Arbáizar, María Patricia; Horcajo Iglesias, María Del Pilar; Jiménez Meléndez, Alejandro; Ortega Mora, Luis Miguel; Collantes Fernández, EstherBovine neosporosis is currently considered one of the main causes of abortion in cattle worldwide and the outcome of the infection is, in part, determined by Neospora caninum isolate virulence. However, the dam and foetal immune responses associated with this factor are largely unknown. We used a model of bovine infection at day 110 of gestation to study the early infection dynamics (10- and 20-days post-infection, dpi) after experimental challenge with high- and low-virulence isolates of N. caninum (Nc-Spain7 and Nc-Spain1H, respectively). In the present work, dam peripheral cellular immune responses were monitored twice a week from -1 to 20 dpi. At different time points, IFN-γ and IL-4 production was investigated in stimulated dam blood and the percentage of monocytes, NK cells, B cells and T cells (CD4+, CD8+ and γδ) in peripheral blood mononuclear cells (PBMC) were determined by flow cytometry. In addition, maternal iliofemoral lymph nodes and foetal spleen and thymus were collected at 10 and 20 dpi for the study of the same cell subpopulations. Peripheral immune response dynamics were similar after the infection with both isolates, with a significant increase in the percentage of CD4+ T cells at 6 and 9 dpi in PBMC, coincident with the higher levels of IFN-γ and IL-4 release. However, the levels of IFN-γ were significantly higher and an increase in CD8+ T cells at 9, 13 and 20 dpi was observed in the dams infected with Nc-Spain7. Nc-Spain1H infection induced higher IL4 levels in stimulated blood and a higher CD4+/CD8+ ratio in PBMC. The analysis of the maternal iliofemoral lymph node showed a significant enhancement in the percentage of NK, CD4+ and CD8+ T cells for the animals infected with the highly virulent isolate and euthanized at 20 dpi. Regarding the foetal responses, the most remarkable result was an increase in the percentage of monocytes at 20 dpi in the spleen of foetuses from both infected groups, which suggests that foetuses were able to respond to N. caninum infection at mid gestation. This work provides insights into how isolate virulence affects the maternal and foetal immune responses generated against N. caninum, which may influence the course of infection.Item Transcriptomics of Besnoitia besnoiti-Infected Fibroblasts Reveals Hallmarks of Early Fibrosis and Cancer Progression(Microorganisms, 2024) Fernández Álvarez, María; Horcajo Iglesias, María Del Pilar; Jiménez Meléndez, Alejandro; Ferré Pérez, Ignacio; Ortega Mora, Luis Miguel; Álvarez García, Gema; Angulo Lara, Pablo; Huertas López, Ana; Huertas López, FranciscoEndothelial injury, inflammatory infiltrate and fibrosis are the predominant lesions in the testis of bulls with besnoitiosis that may result in sterility. Moreover, fibroblasts, which are key players in fibrosis, are parasite target cells in a Besnoitia besnoiti chronic infection. This study aimed to decipher the molecular basis that underlies a drift toward fibrosis during the disease progression. Transcriptomic analysis was developed at two times post-infection (p.i.), representative of invasion (12 h p.i.) and intracellular proliferation (32 h p.i.), in primary bovine aorta fibroblasts infected with B. besnoiti tachyzoites. Once the enriched host pathways were identified, we studied the expression of selected differentially expressed genes (DEGs) in the scrotal skin of sterile infected bulls. Functional enrichment analyses of DEGs revealed shared hallmarks of cancer and early fibrosis. Biomarkers of inflammation, angiogenesis, cancer, and MAPK signaling stood out at 12 h p.i. At 32 h p.i., again MAPK and cancer pathways were enriched together with the PI3K–AKT pathway related to cell proliferation. Some DEGs were also regulated in the skin samples of naturally infected bulls (PLAUR, TGFβ1, FOSB). We have identified potential biomarkers and host pathways regulated during fibrosis that may hold prognostic significance and could emerge as potential therapeutic targets.