Person: Rosales Conrado, Noelia
Loading...
First Name
Noelia
Last Name
Rosales Conrado
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
Identifiers
14 results
Search Results
Now showing 1 - 10 of 14
Item Unravelling the in vitro and in vivo potential of selenium nanoparticles in Alzheimer’s disease: A bioanalytical review(Talanta, 2023) Vicente Zurdo, David; Rosales Conrado, Noelia; León González, María Eugenia DeAlzheimer’s disease (AD) is a devastating neurodegenerative disorder characterized by progressive cognitive decline and the accumulation of beta-amyloid plaques and tau tangles in the brain. Current therapies have limited efficacy, prompting the search for novel treatments. Selenium nanoparticles (SeNPs) have emerged as promising candidates for AD therapy due to their unique physicochemical properties and potential therapeutic effects. This review provides an overview of SeNPs and their potential application in AD treatment, as well as the main bioanalytical techniques applied in this field. SeNPs possess antioxidant and anti-inflammatory properties, making them potential candidates to combat the oxidative stress and neuroinflammation associated with AD. Moreover, SeNPs have shown the ability to cross the blood-brain barrier (BBB), allowing them to target brain regions affected by AD pathology. Various methods for synthesizing SeNPs are explored, including chemical,physical and biological synthesis approaches. Based on the employment of algae, yeast, fungi, and plants, green methods offer a promising and biocompatible alternative for SeNPs production. In vitro studies have demonstrated the potential of SeNPs in reducing beta-amyloid aggregation and inhibiting tau hyperphosphorylation, providing evidence of their neuroprotective effects on neuronal cells. In vivo studies using transgenic mousem models and AD-induced symptoms have shown promising results, with SeNPs treatment leading to cognitive improvements and reduced amyloid plaque burden in the hippocampus. Looking ahead, future trends in SeNPs research involve developing innovative brain delivery strategies to enhance their therapeutic potential, exploring alternative animal models to complement traditional mouse studies, and investigating multi-targeted SeNPs formulations to address multiple aspects of AD pathology. Overall, SeNPs represent a promising avenue for AD treatment, and further research in this field may pave the way for effective and much-needed therapeutic interventions for individuals affected by this debilitating disease.Item Valorization of prunus seed oils: fatty acids composition and oxidative stability(Molecules, 2023) Rodríguez-Blázquez, Sandra; Gómez Mejía, Esther; Rosales Conrado, Noelia; León González, María Eugenia De; García-Sánchez, Beatriz; Miranda Carreño, RubénPrunus fruit seeds are one of the main types of agri-food waste generated worldwide during the processing of fruits to produce jams, juices and preserves. To valorize this by-product, the aim of this work was the nutritional analysis of peach, apricot, plum and cherry seeds using the official AOAC methods, together with the extraction and characterization of the lipid profile of seed oils using GC-FID, as well as the measurement of the antioxidant activity and oxidative stability using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging method. Chemometric tools were required for data evaluation and the obtained results indicated that the main component of seeds were oils (30–38%, w). All seed oils were rich in oleic (C18:1n9c) and linoleic (C18:2n6c) acids and presented heart-healthy lipid indexes. Oil antioxidant activity was estimated in the range IC50 = 20–35 mg·mL−1, and high oxidative stability was observed for all evaluated oils during 1–22 storage days, with the plum seed oil being the most antioxidant and stable over time. Oxidative stability was also positively correlated with oleic acid content and negatively correlated with linoleic acid content. Therefore, this research showed that the four Prunus seed oils present interesting healthy characteristics for their use and potential application in the cosmetic and nutraceutical industries.Item Valorization of citrus reticulata blanco peels to produce enriched wheat bread: phenolic bioaccessibility and antioxidant potential(ANTIOXIDANTS, 2023) Gómez Mejía, Esther; Sacristán, Iván; Rosales Conrado, Noelia; León González, María Eugenia De; Madrid Albarrán, María YolandaThe fortification of foods with bioactive polyphenols aims to improve their functional properties and to provide health benefits. Yet, to exert their benefits, phenolic compounds must be released from the food matrix and absorbed by the small intestine after digestion, so assessing their bioaccessibility is crucial to determine their potential role. This work aims to incorporate Citrus reticulata Blanco peel extracts into wheat bread as a promising opportunity to increase their bioactive potential, along with supporting the sustainable management of citrus-industry waste. A control and a wheat bread enriched at 2% and 4% (w/v) with a phenolic extract from mandarin peels were prepared and analyzed for antioxidant activity and phenolic composition using LC-MS and UV-Vis spectrophotometry. In addition, in vitro digestion was performed, and the digested extracts were analyzed with HPLC-MS/MS. The results showed a significant increase in total flavonoid content (TFC, 2.2 ± 0.1 mg·g−1), antioxidant activity (IC50 = 37 ± 4 mg·g−1), and contents of quercetin, caffeic acid, and hesperidin in the 4% (w/v) enriched bread. Yet, most polyphenols were completely degraded after the in vitro digestion process, barring hesperidin (159 ± 36 μg·g−1), highlighting the contribution of citrus enrichment in the development of an enriched bread with antioxidant potential.Item Phenolic profile, safety, antioxidant and anti-inflammatory activities of wasted Bunium ferulaceum Sm. aerial parts(Food Research International, 2022) Deghima, Amirouche; Righi, Nadjat; Rosales Conrado, Noelia; León González, María Eugenia De; Baali, Faiza; Gómez Mejía, Esther; Madrid Albarrán, María Yolanda; Bedjou, Fatiha; ELSEVIERThe pharmaceutical and nutraceutical industries benefit greatly from recycling and transforming non-utilized parts of medicinal plants from agro-industrial operations into value added products. Hence, the aim of this work was to study the potential nutraceutical and pharmaceutical applications of Bunium ferulaceum Sm. aerial parts, in order to maximize their value. The phenolic profile of their hydromethanolic extract was determined and its antioxidant activity was evaluated in vitro and in vivo alongside with its anti-inflammatory activity and safety profile. The extract exerted an in vitro antioxidant activity mainly through radical scavenging (DPPH IC50: 14.0 ± 0.3 μg/ml) and iron chelating ability (24 ± 2 μg/ml), while, in vivo, the extract did not cause any mortality or visible signs of acute toxicity at high dose (2000 mg/kg body weight). The supplementation of the extract at different doses improved mice liver redox state by increasing catalase and reduced glutathione levels and reducing lipid peroxidation, without causing any toxicity. Moreover, the extract efficiently inhibited xylene induced ear inflammation (62 %). These different bioactivities were linked to the phenolic compounds present in the extract, particularly, chlorogenic acid (78 ± 6 mg/g extract), rutin (44 ± 2 mg/g extract) and hesperidin (56 ± 9 mg/g extract). However, further studies should be carried out on the isolated major compounds found in the extract to correlate the activity with these compounds or their mixture. The wasted aerial parts of Bunium ferulaceum Sm. proved to be a valuable source of polyphenols and exhibited interesting health promoting effects with no toxicity. Thus, Bunium ferulaceum Sm. aerial parts can be included in nutraceutical formulations or used as functional food and the extracted compounds may be used as an alternative food preservative.Item Effect of Storage and Drying Treatments on Antioxidant Activity and Phenolic Composition of Lemon and Clementine Peel Extracts(Molecules, 2023) Gómez Mejía, Esther; Sacristán Navarro, Iván; Rosales Conrado, Noelia; León González, María Eugenia De; Madrid Albarrán, María YolandaObtaining polyphenols from horticultural waste is an emerging trend that enables the valorization of resources and the recovery of value-added compounds. However, a pivotal point in the exploitation of these natural extracts is the assessment of their chemical stability. Hence, this study evaluates the effect of temperature storage (20 and −20 ◦C) and drying methods on the phenolic composition and antioxidant activity of clementine and lemon peel extracts, applying HPLC-DADMS, spectrophotometric methods, and chemometric tools. Vacuum-drying treatment at 60 ◦C proved to be rather suitable for retaining the highest antioxidant activity and the hesperidin, ferulic, and coumaric contents in clementine peel extracts. Lemon extracts showed an increase in phenolic acids after oven-drying at 40 ◦C, while hesperidin and rutin were sustained better at 60 ◦C. Hydroethanolic extracts stored for 90 days preserved antioxidant activity and showed an increase in the total phenolic and flavonoid contents in lemon peels, unlike in clementine peels. Additionally, more than 50% of the initial concentration was maintained up to 51 days, highlighting a half-life time of 71 days for hesperidin in lemon peels. Temperature was not significant in the preservation of the polyphenols evaluated, except for in rutin and gallic acid, thus, the extracts could be kept at 20 ◦C.Item Cytotoxicity, uptake and accumulation of selenium nanoparticles and other selenium species in neuroblastoma cell lines related to Alzheimer’s disease by using cytotoxicity assays, TEM and single cell-ICP-MS(Analytica Chimica Acta, 2023) Vicente Zurdo, David; Gómez Gómez, Beatriz; Romero Sánchez, Iván; Rosales Conrado, Noelia; León González, María Eugenia De; Madrid Albarrán, María Yolanda; Elsevier B.V.Alzheimer’s disease (AD) is the most prevalent neurodegenerative disease, representing 80% of the total dementia cases. The “amyloid cascade hypothesis” stablishes that the aggregation of the beta-amyloid protein (Aβ42) is the first event that subsequently triggers AD development. Selenium nanoparticles stabilized with chitosan (Ch-SeNPs) have demonstrated excellent anti-amyloidogenic properties in previous works, leading to an improvement of AD aetiology. Here, the in vitro effect of selenium species in AD model cell line has been study to obtain a better assessment of their effects in AD treatment. For this purpose, mouse neuroblastoma (Neuro-2a) and human neuroblastoma (SH-SY5Y) cell lines were used. Cytotoxicity of selenium species, such as selenomethionine (SeMet), Se-methylselenocysteine (MeSeCys) and Ch-SeNPs, has been determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry methods. Intracellular localisation of Ch-SeNPs, and their pathway through SH-SY5Y cell line, have been evaluated by transmission electron microscopy (TEM). The uptake and accumulation of selenium species by both neuroblastoma cell lines have been quantified at single cell level by single cell- Inductively Coupled Plasma with Mass Spectrometry detection (SCICP-MS), with a previous optimisation of transport efficiency using gold nanoparticles (AuNPs) ((69 ± 3) %) and 2.5 mm calibration beads ((92 ± 8) %). Results showed that Ch-SeNPs would be more readily accumulated by both cell lines than organic species being accumulation ranges between 1.2 and 89.5 fg Se cell 1 for Neuro-2a and 3.1–129.8 fg Se cell1 for SH-SY5Y exposed to 250 μM Ch-SeNPs. Data obtained were statistically using chemometric tools. These results provide an important insight into the interaction of Ch-SeNPs with neuronal cells, which could support their potential use in AD treatment.Item Bioactive polyphenols from Ranunculus macrophyllus Desf. Roots: quantification, identification and antioxidant activity(South African Journal of Botany, 2020) Deghima, Amirouche; Righi, Nadjat; Rosales Conrado, Noelia; León González, María Eugenia De; Gómez Mejía, Esther; Madrid Albarrán, María Yolanda; Baali, Faiza; Bedjou, FatihaRanunculus macrophyllus Desf. is an Algerian medicinal plant whose roots are used in traditional medicine to cure feminine infertility and other diseases, however there are no studies regarding its phytochemistry and biological activities. The aim of this work is to study the phytochemical composition and antioxidant activity of different solvents fractions from the roots of Ranunculus macrophyllus Desf. Spectrophotometric and chromatographic methods were used to study the phytochemical composition; while antiradical, iron chelating ability, reducing power and lipid peroxidation were studied in-vitro. The ethyl acetate fraction showed the highest values of total phenolic compounds (271.0 ± 0.2 µg GAE/mg dry extract (d.e), flavonols (24 ± 5 µg RE/ mg d.e) and condensed tannins (129 ± 10 µg CE/mg d.e), while the hexane fraction contained the highest amount of triterpenoids (91 ± 7 µg UAE/mg d.e). The highest radical scavenging ability was recorded for the ethyl acetate fraction against DPPH (IC50 = 3.7 ± 0.1 µg/mL) and ABTS (IC50 = 81 ± 3 µg/mL) whereas the hexane fraction had the best hydrogen peroxide radical scavenging (IC50 = 380 ± 4 µg/mL). The ethyl acetate fraction had the best total antioxidant capacity (TAC = 361 ± 1 µAAE/ mg extract) and reducing power (310 ± 2 µAAE/mg extract). The β-carotene bleaching was inhibited at high rate even after 24 h by the ethyl acetate fraction (81.0 ± 0.5 %). All activities were correlated with the polyphenolic content of the fractions. Capillary LC-DAD and LC-MS/MS analysis of ethyl acetate fraction revealed high amounts of gallic acid (9.3 ± 0.6 mg/g d.e), dihydroxybenzoic acid (8.1 ± 0.2 mg/g d.e) and hesperidin (5.9 ± 0.6 mg/g d.e). With such high amounts of polyphenols and strong antioxidant activity Ranunculus macrophyllus Desf. roots could have a potential use in pharmaceutical and nutraceutical industries.Item Valorization of defatted cherry seed residues from Liquor processing by matrix solid-phase dispersion extraction: a sustainable strategy for production of phenolic-rich extracts with antioxidant potential(Antioxidants, 2023) Rodríguez-Blázquez, Sandra; Fernández-Ávila, Lorena; Gómez Mejía, Esther; Rosales Conrado, Noelia; León González, María Eugenia De; Miranda Carreño, RubénThe integrated valorization of food chain waste is one of the most promising alternatives in the transition to a sustainable bioeconomy. Thus, an efficient solid-phase matrix dispersion extraction method, using experimental factorial design and response surface methodology, has been developed and optimized for the recovery of polyphenols from defatted cherry seeds obtained after cherry liquor manufacture and subsequent fatty acid extraction, evaluating the effect of each processing step on the composition and phenolic content of sweet cherry residues. The phenolic extracts before fermentation showed the highest content of total polyphenols (TPC) and flavonoids (TFC) (3 ± 1 mg QE·g−1 and 1.37 ± 0.08 mg GAE·g−1, respectively), while the highest antioxidant capacity was obtained in the defatted seed extracts after both fermentation and distillation. In addition, high-performance liquid chromatography coupled to a quadrupole time-of-flight mass spectrometer (HPLC-ESI-QTOF-MS) was used to determine the phenolic profile. Dihydroxybenzoic acid, neochlorogenic acid, caffeic acid, and quercetin were the main phenolics found, showing differences in concentration between the stages of liquor production. The results underline the prospective of cherry by-products for obtaining phenol-rich bioactive extracts for possible use in different industrial sectors, offering a feasible solution for the cascade valorization of cherry agri-food waste.Item Ability of selenium species to inhibit metal-induced Aβ aggregation involved in the development of Alzheimer’s disease(Analytical and Bioanalytical Chemistry, 2020) Vicente Zurdo, David; Romero Sánchez, Iván; Rosales Conrado, Noelia; León González, María Eugenia De; Madrid Albarrán, María Yolanda; Springer HeidelbergExtracellular accumulation of amyloid beta peptide (Aβ) is believed to be one of the main factors responsible for neurodegeneration in Alzheimer’s disease (AD). Metals could induce Aβ aggregation, by their redox activity or binding properties to amyloid β fibrils, leading to their accumulation and deposition outside neurons. For this reason, metal chelation may have an acknowledged part to play in AD prevention and treatment. In the current work, the role of different selenium species, including selenium nanoparticles, in Aβ aggregation, was studied by evaluating their metal-chelating properties and their ability both to inhibit metal-induced Aβ1–42 aggregation fibrils and to disaggregate them once formed. Transition biometals such as Fe(II), Cu(II), and Zn(II) at 50 μM were selected to establish the in vitro models. The DPPH assay was used to determine the antioxidant capacity of the evaluated selenium species. Selenium nanoparticles stabilized with chitosan (Ch-SeNPs) and with both chitosan and chlorogenic acid polyphenol (CGA@ChSeNPs) showed the highest antioxidant properties with EC50 of 0.9 and 0.07 mM, respectively. UV–Vis and d1(UV–Vis) spectra also revealed that selenium species, in particular selenomethionine (SeMet), were able to interact with metals. Regarding Aβ1–42 incubation experiments, Fe(II), Cu(II), and Zn(II) induced Aβ aggregation, in a similar way to most of the evaluated selenium species. However, Ch-SeNPs produced a high inhibition of metal-induced Aβ aggregation, as well as a high disaggregation capacity of Aβ fibrils in both the presence and absence of biometals, in addition to reducing the length and width (20% of reduction in the presence of Zn(II)) of the generated Aβ fibrils.Item Anti-inflammatory activity of ethyl acetate and n-butanol extracts from Ranunculus macrophyllus Desf. and their phenolic profile(Journal of Ethnopharmacology, 2021) Deghima, Amirouche ; Righi, Nadjat; Rosales Conrado, Noelia; León González, María Eugenia De; Baali, Faiza; Gómez Mejía, Esther; Madrid Albarrán, María Yolanda; Bedjou, FatihaEthnopharmacological relevance: The members of the genus Ranunculus have counter-irritating properties and thus, they are traditionally used for treating anti-inflammatory disorders and other skin conditions. Ranunculus macrophyllus Desf. is a wild medicinal plant growing in Algeria and traditionally used to treat some cutaneous skin disorders. Aim: The aim of this study was to characterize the composition of the ethyl acetate and n-butanol extracts from Ranunculus macrophyllus Desf. as well as to elucidate and to compare their effect against acute skin inflammation. Moreover, both the antioxidant activity and the acute toxicity of the plant extracts were also studied. Materials and methods: Spectrophotometric and chromatographic methods were employed to identify and quantify phenolic compounds and triterpenoids from R. macrophyllus Desf. fractions. The antioxidant activity was estimated using the phosphomolebdenum, DPPH, reducing power and β-carotene bleaching assays. The ethyl acetate and n-butanol extracts were screened for their anti-inflammatory activities using ex-vivo membrane stabilizing assays and in-vivo acute skin inflammation model. Results: Ethyl acetate fraction showed the highest amounts of total phenolic compounds (413 ± 4 μg GAE/mg extract) and triterpenoids (70.4 ± 1.8 μg UAE/mg extract). Rutin, hesperidin, myricetin and kaempferol were the major compounds identified in the different fractions. Ethyl acetate fraction exhibited strong DPPH• radical scavenging ability (IC50 1.6 ± 0.2 μg/mL), high total antioxidant capacity (447 ± 7 μg AAE/mg extract) and reducing power (514 ± 8 μg AAE/mg extract). Ethyl acetate fraction inhibited (73.4 ± 0.3) % of linoleic acid peroxidation. Ethyl acetate and n-butanol fractions did not have any visible toxicity at 2000 mg/kg and presented excellent membrane stabilizing ability. The inhibition of xylene induced ear inflammation was (38 ± 4) % and (46 ± 1) % for RM-B and RM-EA, respectively. Conclusions: The high content of both phenolic compounds and triterpenoids combined with the remarkable antiinflammatory effect and antioxidant activity of ethyl acetate and n-butanol extracts from R. macrophyllus Desf. support the wide spread use of this traditional plant on some skin disorders (inflammatory skin disorders).