Person:
Rosales Conrado, Noelia

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First Name
Noelia
Last Name
Rosales Conrado
URI
https://hdl.handle.net/20.500.14352/76222
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
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2020 - 20213
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End date: 2023 × Subject: 54 ×

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    Integration of untargeted and targeted mass spectrometry-based metabolomics provides novel insights into the potential toxicity associated to surfynol
    (Food and Chemical Toxicology, 2020) Garcia-Calvo, Estefania; Machuca Marcos, Andrés; Nerin, Cristina; Rosales Conrado, Noelia; Anunciacao, Daniela S; Luque García, José Luis
    The intake of toxic compounds through the diet as a result of migration processes from food packaging is of increasing concern. It has been shown that the surfactant commercially known as surfynol, which is commonly used in food-contact materials, is capable of migrating from multilayer containers into the food, reaching potentially harmful concentration levels. In the present study, the integration of an untargeted and a targeted metabolomics approach has been carried out using NTERA-2 germinal cells as in-vitro model, to make further progress in elucidating the molecular mechanisms associated with the toxicity of surfynol. This study has allowed the identification of different altered metabolites mainly related with energy-acquiring, cell development and cellular defense mechanisms. While glutamine, L-threonine, propanoate, octadecanoate and carbamate were found at higher concentrations in cells exposed tu surfynol, L-valine, oxalate, phosphate, phenylalanine and myoinositol were found inhibited. Additionally, concentrations of ATP, ADP and NAD+ were found significantly inhibited, supporting the idea that surfynol induces glycolysis inactivation. The results obtained strengthen the evidence of the toxicity associated to surfynol; therefore, reinforcing the need for a more comprehensive study on the viability of its use in food packaging.
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    Aprendizaje de las reacciones químicas en Educación Secundaria a través de actividades cooperativas
    (Educación Química, 2021) Peña Martínez, Juan; Pérez López, Raquel; Rosales Conrado, Noelia
    En el presente trabajo se propone una secuencia didáctica para la enseñanza de las reacciones químicas en educación secundaria, que incluye dos actividades diferentes de aprendizaje cooperativo. La primera, para trabajar la diferencia entre cambio físico y cambio químico y, la segunda, para consolidar la parte experimental que también se ha llevado a cabo en el laboratorio. Un total de 43 estudiantes valoraron la experiencia y la metodología empleada mediante un cuestionario. Los resultados muestran que aproximadamente un 80% están a favor de emplear el modelo de aprendizaje con grupos cooperativos. No obstante, un 30% de los estudiantes señalan que hay que asegurar que todos los miembros de los equipos participen y realicen el trabajo asignado. La metodología cooperativa se podría emplear en la enseñanza de las reacciones químicas.
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    Valorisation of black mulberry and grape seeds: Chemical characterization and bioactive potential
    (Food Chemistry, 2021) Gómez Mejía, Esther; Lobo Roriz, Custódio ; Heleno, Sandrina ; Calhelha, Ricardo ; Dias, Maria Inês ; Pinela, José; Rosales Conrado, Noelia; León González, María Eugenia De; Ferreira, Isabel; Barros, Lillian
    Grape (Vitis vinifera L. var. Albariño) and mulberry (Morus nigra L.) seeds pomace were characterized in terms of tocopherols, organic acids, phenolic compounds and bioactive properties. Higher contents of tocopherols (28 ± 1 mg/100 g fw) were obtained in mulberry, whilst grape seeds were richer in organic acids (79 ± 4 mg/100 g fw). The phenolic analysis of hydroethanolic extracts characterised grape seeds by catechin oligomers (36.0 ± 0.3 mg/g) and mulberry seeds by ellagic acid derivatives (3.14 ± 0.02 mg/g). Both exhibited high antimicrobial activity against multiresistant Staphylococcus aureus MIC = 5 mg/mL) and no cytotoxicity against carcinogenic and non-tumour primary liver (PLP) cells. Mulberry seeds revealed the strongest inhibition (p < 0.05) against thiobarbituric reactive substances (IC50 = 23 ± 2 µg/mL) and oxidative haemolysis (IC50 at 60 min = 46.0 ± 0.8 µg/mL). Both seed by-products could be exploited for the developing of antioxidant-rich ingredients with health benefits for industrial application.