Person: Meana Mañes, María Aranzazu
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First Name
María Aranzazu
Last Name
Meana Mañes
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
4 results
Search Results
Now showing 1 - 4 of 4
Item Age and Method of Inoculation Influence the Infection of Worker Honey Bees (Apis mellifera) by Nosema ceranae(Insects, 2019) Urbieta-Magro, Almudena; Higes, Mariano; Meana Mañes, María Aranzazu; Barrios, Laura; Martín-Hernández, RaquelThe microsporidian parasite Nosema ceranae is a highly prevalent, global honey bee pathogen. Apis mellifera is considered to be a relatively recent host for this microsporidia, which raises questions as to how it affects its host’s physiology, behavior and longevity, both at the individual and colony level. As such, honey bees were inoculated with fresh purified spores of this pathogen, both individually (Group A) or collectively (Group B) and they were studied from 0 to 15 days post-emergence (p.e.) to evaluate the effect of bee age and the method of inoculation at 7 days post-infection. The level of infection was analyzed individually by qPCR by measuring the relative amount of the N. ceranae polar tubule protein 3 (PTP3) gene. The results show that the bee’s age and the method of infection directly influence parasite load, and thus, early disease development. Significant differences were found regarding bee age at the time of infection, whereby the youngest bees (new-born and 1 day p.e.) developed the highest parasite load, with this load decreasing dramatically in bees infected at 2 days p.e. before increasing again in bees infected at 3–4 days p.e. The parasite load in bees infected when older than 4 days p.e. diminished as they aged. When the age cohort data was pooled and grouped according to the method of infection, a significantly higher mean concentration and lower variation in N. ceranae infection was evident in Group A, indicating greater variation in experimental infection when spores were administered collectively to bees through their food. In summary, these data indicate that both biological and experimental factors should be taken into consideration when comparing data published in the literature.Item Genetic profile of Varroa destructor infesting Apis mellifera iberiensis colonies(Journal of Apicultural Research, 2008) Muñoz Gabaldón, Irene; Garrido Bailón, Encarna; Martín Hernández, Raquel; Meana Mañes, María Aranzazu; Higes, Mariano; De la Rúa, PilarThe genetic profile of the Varroa destructor mite infesting Apis mellifera iberiensis colonies located in the Iberian Peninsula and also on Canarian and Balearic islands was determined through standard molecular assays (RFLP of the mitochondrial cox1 fragment). The V. destructor Korea haplotype was found in all of the 575 samples analyzed except in one, confirming the worldwide expansion of the more virulent haplotype. The mitochondrial haplotypes of the honey bees from an apiary where the Japan haplotype was detected were also determined, but no significant relationship was observed in the parasite-host haplotype distribution.Item Epidemiology of the Microsporidium Nosema ceranae in Four Mediterranean Countries(Insects, 2022) Jabal Uriel, Clara; Barrios, Laura; Bonjour Dalmon, Anne; Caspi Yona, Shiran; Chejanovsly, Nor; Erez, Tal; Henriques, Dora; Higes, Mariano; Le Conte, Yves; Lopes, Ana R.; Meana Mañes, María Aranzazu; Pinto, Maria Alice; Reyes Carreño, Maritza; Soroker, Victoria; Martín Hernández, RaquelNosema ceranae is a highly prevalent intracellular parasite of honey bees’ midgut worldwide. This Microsporidium was monitored during a long-term study to evaluate the infection at apiary and intra-colony levels in six apiaries in four Mediterranean countries (France, Israel, Portugal, and Spain). Parameters on colony strength, honey production, beekeeping management, and climate were also recorded. Except for São Miguel (Azores, Portugal), all apiaries were positive for N. ceranae, with the lowest prevalence in mainland France and the highest intra-colony infection in Israel. A negative correlation between intra-colony infection and colony strength was observed in Spain and mainland Portugal. In these two apiaries, the queen replacement also influenced the infection levels. The highest colony losses occurred in mainland France and Spain, although they did not correlate with the Nosema infection levels, as parasitism was low in France and high in Spain. These results suggest that both the effects and the level of N. ceranae infection depends on location and beekeeping conditions. Further studies on host-parasite coevolution, and perhaps the interactions with other pathogens and the role of honey bee genetics, could assist in understanding the difference between nosemosis disease and infection, to develop appropriate strategies for its control.Item Diferencias morfométricas entre la abeja Apis mellifera iberica y la abeja rusa de la región de Primorsky(Revista Complutense de Ciencias Veterinarias, 2007) Fontanillas Pérez, Juan Carlos; Higes, Mariano; García-Cuenca Ariati, Isabel; Meana Mañes, María Aranzazu; Mogedas Moreno, MaríaLas abejas rusas de la región de Primorsky se ha demostrado que poseen una gran tolerancia a Varroa. En el presente trabajo hemos realizado un estudio comparativo de estas abejas y Apis mellifera iberica, mediante un estudio morfométrico, sometiendo los valores obtenidos a un estudio estadístico descriptivo, evaluando la normalidad de la distribución mediante el test de Kolmogorov-Smirnov y las diferencias entre las dos poblaciones de abeja mediante tests paramétricos (t´Student) o no paramétricos (U de Mann-Whitney), según el tipo de distribución.