Person:
Benito Peña, María Elena

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First Name
María Elena
Last Name
Benito Peña
URI
https://hdl.handle.net/20.500.14352/76070
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
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    Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone
    (Biosensors and Bioelectronics, 2020) Peltomaa, Riikka Johanna; Farka, Zdeněk; Mickert, Matthias ; Brandmeier, Julian ; Pastucha, Matěj; Hlaváček, Antonín; Martínez Orts, Mónica; Canales Mayordomo, María Ángeles; Skládal, Petr; Benito Peña, María Elena; Moreno Bondi, María Cruz; Gorris, Hans
    Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) for the analysis of zearalenone (ZEA), one of the most frequently encountered mycotoxins in food worldwide. Instead of a toxin-conjugate conventionally used in competitive immunoassays, we designed a ZEA mimicking peptide extended by a biotin-linker and confirmed its excellent suitability to mimic ZEA by nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) analysis. Upconversion nanoparticles (UCNP, type NaYF4:Yb,Tm) served as background-free optical label for the detection of the peptide mimetic in the competitive ULISA. Streptavidin-conjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. The UCNP conjugate clearly outperformed conventional labels such as enzymes or fluorescent dyes. With a limit of detection of 20 pg mL−1 (63 pM), the competitive ULISA is well applicable to the detection of ZEA at the levels set by the European legislation. Moreover, the ULISA is specific for ZEA and its metabolites (α- and β-zearalenol) without significant cross-reactivity with other related mycotoxins. We detected ZEA in spiked and naturally contaminated maize samples using liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) as a reference method to demonstrate food analysis in real samples.