Person:
Cintas Izarra, Luis Miguel

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First Name
Luis Miguel
Last Name
Cintas Izarra
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Nutrición y Ciencia de los Alimentos
Area
Nutrición y Bromatología
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    Identification of bacteriocin genes in enterococci isolated from game animals and saltwater fish
    (Journal of Food Protection, 2011) Almeida, Tereza; Brandão, Andreia; Muñoz Atienza, Estefanía; Gonçalves, Alexandre; Torres, Carmen; Igrejas, Gilberto; Hernández Cruza, Pablo Elpidio; Herranz Sorribes, Carmen; Cintas Izarra, Luis Miguel; Poeta, Patrícia
    Bacteriocins produced by enterococci, referred to as enterocins, possess great interest for their potential use as biopreservatives in food and feed, as well as alternative antimicrobials in humans and animals. In this context, the aim of the present study was to determine the antimicrobial activity and the presence of bacteriocin structural genes in fecal enterococcal isolates from animal origins. Evaluation of the direct antimicrobial activity of 253 isolates from wild boars (Sus scrofa, n = 69), mullets (Liza ramada, n = 117), and partridges (Perdix perdix, n = 67) against eight indicator bacterial strains (including Listeria monocytogenes, Pediococcus pentosaceus, and Enterococcus spp.) showed that 177 (70%) exerted antimicrobial activity against at least one indicator microorganism. From these isolates, 123 were further selected on the basis of their inhibition group, and 81 were found to be producers of bacteriocins active against Listeria monocytogenes. Analysis of the presence of enterocin structural genes in a subset of 36 isolates showed that 70% harbored one or more of the evaluated genes, those of enterocin P and hiracin JM79 being the most prevalent. These results show that wild animals constitute an appropriate source for the isolation of bacteriocinogenic enterococci.