Person:
Calvo Garrido, María Lourdes

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First Name
María Lourdes
Last Name
Calvo Garrido
URI
https://hdl.handle.net/20.500.14352/76255
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Ingeniería Química y de Materiales
Area
Ingeniería Química
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    Inactivation of clostridium spores in honey with supercritical CO2 and in combination with essential oils
    (Processes, 2022) Dacal-Gutiérrez, Alejandro; Tirado, Diego ; Calvo Garrido, María Lourdes
    The presence of tens of Clostridium botulinum spores per gram of honey can cause infantile botulism. Thermal treatment is insufficient to inactivate these resistant forms. This study explored the effectiveness of supercritical CO2 (scCO2) on its own and combined with lemon (LEO), clove (CLEO), and cinnamon (CEO) essential oils on the inactivation of Clostridium sporogenes (CECT 553) as a surrogate of Clostridium botulinum. In water, the degree of inactivation at 10 MPa after 60 min increased with the increasing temperature, reducing the population by 90% at 40 °C and by 99.7% at 80 °C. In contrast, when applied to honey, scCO2 did not inactivate Clostridium spores satisfactorily at temperatures below 70 °C, which was related to the protective effect of honey. Meanwhile, scCO2 modified with CEO (<0.4% mass) improved the inactivation degree, with a 1.3-log reduction achieved at 60 °C. With this same mixture, a reduction of 3.7 logs was accomplished in a derivative with 70% moisture. Honey was very sensitive to the temperature of the applied CO2. The obtained product could be used as a novel food, food ingredient, cosmetic, or medicine.
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    Project number: 172
    Formación en Química Sostenible y su relación con los Objetivos de Desarrollo Sostenible
    () Cabañas Poveda, Albertina; Pérez Velilla, Eduardo; Vázquez Villa, María Del Henar; García Melo, Fátima; Calvo Garrido, María Lourdes; González Mac-Dowell, Luis; Acción Salas, Fernando; Parrón Nieto, Andrea; Valero Herrero, Patricia
    En el proyecto se propone la realización de trabajos en grupo dirigidos a los alumnos de Química General de 1º en los que se desarrollen los principios de la Química Sostenible y se relacionen con los Objetivos de Desarrollo Sostenible. Las mejores presentaciones serán grabadas en video para su difusión.