Person:
Ortega Medina, Luis

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First Name
Luis
Last Name
Ortega Medina
URI
https://hdl.handle.net/20.500.14352/79235
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Medicina Legal, Psiquiatría y Patología
Area
Anatomía Patológica
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20222
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Author: Arcos Navarrete, Daniel ×

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    In Vitro and In Vivo Response of Zinc-Containing Mesoporous Bioactive Glasses in a Sheep Animal Model
    (International Journal of Molecular Sciences, 2022) Jiménez Holguín, Javier; Arcos Navarrete, Daniel; Lozano Borregón, Daniel; Sainz-Pardo Sanz, Melchor; Ortega Medina, Luis; Enciso, S; Fernández Tomé, Blanca; López Güemes, Idoia; Sánchez Margallo, Francisco Miguel; Casarrubios Molina, Laura; Portolés Pérez, María Teresa; Vallet Regí, María Dulce Nombre
    Zinc-enriched mesoporous bioactive glasses (MBGs) are bioceramics with potential antibacterial and osteogenic properties. However, few assays have been performed to study these properties in animal models.In this study, MBGs enriched with up to 5% ZnO were synthesized, physicochemically characterized, and evaluated for their osteogenic activity both in vitro and in vivo. The ZnO MBGs showed excellent textural properties despite ZnO incorporation. However, the release of Zn2+ ions inhibited the mineralization process when immersed in simulated body fluid. In vitro assays showed significantly highe r values of viability and expression of early markers of celldifferentiation and angiogenesis in a ZnO-content-dependent manner. The next step was to study the osteogenic potential in a sheep bone defect model. Despite their excellent textural properties and cellular response in vitro, the ZnO MBGs were not able to integrate into the bone tissue, which can be explained in terms of inhibition of the mineralization process caused by Zn2+ ions. This work highlights the need to develop nanostructured materials for bone regeneration that can mineralize to interact with bone tissue and induce the processes of implant acceptance, cell colonization by osteogenic cells, and regeneration of lost bone tissue.
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    Injectable mesoporous bioactive nanoparticles regenerate bone tissue under osteoporosis conditions
    (Acta Biomaterialia, 2022) Arcos Navarrete, Daniel; Gómez Cerezo, María Natividad; Saiz-Pardo Sanz, Melchor; Pablo Velasco, David de; Ortega Medina, Luis; Enciso, Silvia; Fernández Tomé, Blanca; Díaz Güemes, Idoia; Sánchez Margallo, Francisco Miguel; Casarrubios Palomar, Luis; Feito Castellano, María José; Portolés Pérez, María Teresa; Vallet Regí, María Dulce Nombre
    The osteogenic capability of mesoporous bioactive nanoparticles (MBNPs) in the SiO2–CaO system has been assessed in vivo using an osteoporotic rabbit model. MBNPs have been prepared using a double template method, resulting in spherical nanoparticles with a porous core-shell structure that has a high surface area and the ability to incorporate the anti-osteoporotic drug ipriflavone. In vitro expression of the pro-inflammatory genes NF-κB1, IL-6, TNF-α, P38 and NOS2 in RAW-264.7 macrophages, indicates that these nanoparticles do not show adverse inflammatory effects. An injectable system has been prepared by suspending MBNPs in a hyaluronic acid-based hydrogel, which has been injected intraosseously into cavitary bone defects in osteoporotic rabbits. The histological analyses evidenced that MBNPs promote bone regeneration with a moderate inflammatory response. The incorporation of ipriflavone into these nanoparticles resulted in a higher presence of osteoblasts and enhanced angiogenesis at the defect site, but without showing significant differences in terms of new bone formation.