Person: Gómez-Lus Centelles, María Luisa
Loading...
First Name
María Luisa
Last Name
Gómez-Lus Centelles
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Medicina
Area
Microbiología
Identifiers
5 results
Search Results
Now showing 1 - 5 of 5
Item Comparing rubbing and scrubbing surgical hand antisepsis with propan-1-ol 60% in accordance with European regulation UNE-EN 12791:2016+A1:2018(Infection Control & Hospital Epidemiology, 2021) Martin Villa, Carlos; Becerro De Bengoa Vallejo, Ricardo; Alou Cervera, Luis; González Hidalgo, Natalia; Losa Iglesias, Marta; Gómez-Lus Centelles, María Luisa; Sevillano Fernández, David; Cambridge University PressA crossover trial was conducted to compare hand rub and hand scrub-brush methods for reducing bacterial loads when using propan-1-ol-60% according to European regulations. Both methods significantly reduced the bacterial load immediately after antisepsis, but only the hand rub method achieved significant bacterial load reduction 3 hours after the procedure.Item Effect on the reduction of bacterial load after surgical hand antisepsis with triclosan 0.5% compared to triclosan 0.5% followed by 70% alcoholic solution(Infection Control & Hospital Epidemiology, 2021) Santé Serna, Luis Narciso; Gómez-Lus Centelles, María Luisa; Martin Villa, Carlos; Becerro De Bengoa Vallejo, Ricardo; Alou Cervera, Luis; Sevillano Fernández, DavidTriclosan 0.5% by scrubbing does not meet the UNE-EN12791 criteria to be used in the surgical hand preparation (SHP). Triclosan 0.5% by scrubbing followed by ethanol 70% hand rubbing is suitable without the additional characteristic of sustained effect. This limited effectiveness implies that triclosan should be avoided in SHP given the restrictions on its use in consumer antiseptic products. The trial was registered at ClinicalTrials.gov (ID: NCT04538365).Item Estudiando la microbiota a través de la prevalencia de portadores nasales de Staphylococcus aureus en los estudiantes de Microbiología del Grado de Podología(European Journal of Podiatry, 2020) Gómez-Lus Centelles, María Luisa; Ciudad Cabañas, María José; Collado Yurrita, Luis Rodolfo; Sevillano Fernández, David; González Hidalgo, Natalia; Alou Cervera, LuisObjetivos. Se ha planteado como objetivo la mejora de la calidad de la docencia de la Microbiología mediante la actualización de la metodología docente, introduciendo como actividad el aprendizaje activo basado en la detección de portadores nasales de Staphylococcus aureus para conseguir mejorar las competencias que deberán adquirir los estudiantes como parte de su formación integral. Material y métodos. En este estudio han participado 56 y 72 alumnos (128 alumnos en total) del Grado de Podología de la Universidad Complutense de Madrid de los cursos 2018-2019 y 2019-2020, respectivamente. Los alumnos asistieron al laboratorio de Microbiología y completaron el estudio. Resultados. Un total de 29 alumnos (22,7%) fueron portadores nasales de S. aureus, uno de los cuales fue portador de S. aureus resistente a meticilina (SARM). La valoración de la actividad por parte de los alumnos fue muy positiva y se produjo una mejora general en la adquisición de conocimientos. Conclusión. El trabajo en el laboratorio de Microbiología unido a la actualización en la metodología docente puede mejorar el rendimiento académico de los estudiantes de Microbiología al relacionarlo con su futura práctica profesional y el diagnóstico microbiológico.Item Prevalence of Staphylococcus spp. nasal colonization among doctors of podiatric medicine and associated risk factors in Spain(Antimicrobial Resistance & Infection Control, 2018) Benito, Sheila de; Alou Cervera, Luis; Becerro De Bengoa Vallejo, Ricardo; Losa Iglesias, Marta Elena; Gómez-Lus Centelles, María Luisa; Collado Yurrita, Luis Rodolfo; Sevillano Fernández, DavidBackground: This study aimed to estimate the prevalence of methicillin-susceptible and -resistant Staphylococcus aureus (MSSA and MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE) nasopharyngeal carriage among Doctors of Podiatric Medicine (Podiatrists) and to determine the potential risk factors. Methods: A cross-sectional study was carried out in 2016-2017 among 239 podiatrists in Spain. The presence of MSSA, MRSA, and MRSE was determined by microbiological analysis of nasal exudate and antimicrobial susceptibility was determined. Each podiatrist completed a questionnaire. The questionnaire comprised various parameters such as sex, age, podiatry experience duration, underlying diseases, prior antibiotic treatment, hospitalization during the last year, and use of a protective mask, an aspiration system, or gloves. Results: The prevalence of MSSA, MRSA, and MRSE was 23.0%, 1.3%, and 23.8%, respectively. The MSSA prevalence was higher among podiatrists who did not use an aspiration system (32.3%) compared to those who did (19.3%; p = 0.0305), and among podiatrists with respiratory diseases (36.8%) compared to those without (20.8%; p = 0.0272). The MRSE prevalence was higher among men (33.7%) compared to women (8.6%; p = 0.0089), podiatrists aged ≥50 (38.5%) compared to ≤35 (17.8%; p = 0.0101), and podiatrists with ≥15 (39.3%) compared to ≤5 years of podiatry experience (12.5%; p = 0.0015). Among the S. aureus strains, 84.5% were resistant to penicillin, 22.4% to erythromycin, 20.7% to clindamycin, and 12.7% to mupirocin. The MRSE strains were resistant to penicillin (93.0%), erythromycin (78.9%), and mupirocin (73.7%). Conclusions: The prevalence of S. aureus and S. epidermidis nasal carriage is low among Spanish podiatrists compared to other health professionals.Item Changes in brain kynurenine levels via gut microbiota and gut‐barrier disruption induced by chronic ethanol exposure in mice(The FASEB Journal, 2019) Giménez Gómez, Pablo; Pérez Hernández, Mercedes; O'Shea Gaya, María Esther; Caso Fernández, Javier Rubén; Martín Hernández, David; Alou Cervera, Luis; Gómez-Lus Centelles, María Luisa; Gutiérrez López, María Dolores; Colado Megías, María IsabelInflammatory processes have been shown to modify tryptophan (Trp) metabolism. Gut microbiota appears to play a significant role in the induction of peripheral and central inflammation. Ethanol (EtOH) exposure alters gut permeability, but its effects on Trp metabolism and the involvement of gut microbiota have not been studied. We analyzed several parameters of gut-barrier and of peripheral and central Trp metabolism following 2 different EtOH consumption patterns in mice, the binge model, drinking in the dark (DID), and the chronic intermittent (CI) consumption paradigm. Antibiotic treatment was used to evaluate gut microbiota involvement in the CI model. Mice exposed to CI EtOH intake, but not DID, show bacterial translocation and increased plasma LPS immediately after EtOH removal. Gut-barrier permeability to FITC-dextran is increased by CI, and, furthermore, intestinal epithelial tight-junction (TJ) disruption is observed (decreased expression of zonula occludens 1 and occludin) associated with increased matrix metalloproteinase (MMP)-9 activity and iNOS expression. CI EtOH, but not DID, increases kynurenine (Kyn) levels in plasma and limbic forebrain. Intestinal bacterial decontamination prevents the LPS increase but not the permeability to FITC-dextran, TJ disruption, or the increase in MMP-9 activity and iNOS expression. Although plasma Kyn levels are not affected by antibiotic treatment, the elevation of Kyn in brain is prevented, pointing to an involvement of microbiota in CI EtOH-induced changes in brain Trp metabolism. Additionally, CI EtOH produces depressive-like symptoms of anhedonia, which are prevented by the antibiotic treatment thus pointing to an association between anhedonia and the increase in brain Kyn and to the involvement of gut microbiota.-Giménez-Gómez, P., Pérez-Hernández, M., O'Shea, E., Caso, J. R., Martín-Hernández, D., Cervera, L. A., Centelles. M. L. G.-L., Gutiérrez-Lopez, M. D., Colado, M. I. Changes in brain kynurenine levels via gut microbiota and gut-barrier disruption induced by chronic ethanol exposure in mice.