Person: Blanco Gutiérrez, María Del Mar
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First Name
María Del Mar
Last Name
Blanco Gutiérrez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
2 results
Search Results
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Item Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells(Comparative Immunology, Microbiology and Infectious Diseases, 2017) Carrión Herrero, Francisco Javier; Domínguez Bernal, Gustavo Ramón; Martínez Rodrigo, Abel; Mas Zubiri, Alicia; Blanco Gutiérrez, María Del Mar; Orden Gutiérrez, José Antonio; Fuente López, Ricardo De LaHere, we describe a novel approach that exploits an attenuated mutant of Salmonella enterica serovar Choleraesuis as carrier to deliver a plasmid encoding protein HisAK70. Subsequently, dendritic cells (DCs) were pulsed with this vaccine vector. The aim of this study was to evaluate the effectiveness of the prepared HisAK70-S. Choleraesuis-pulsed DCs (HisAK70-SAL DCs) against visceral leishmaniosis (VL). In our ex vivo model of infection, the prepared formulations could decrease parasite growth by up to 80% by augmenting the production of IL-12p40 and by reducing arginase activity (ARG). Also, BALB/c mice when immunised with this formulation showed significant reduction in parasite burden in both spleen (20% of reduction) and liver (75% of reduction). The balance of the immune ratios IFN-γ/IL-10, TNF-α/IL-10, and IgG2a/IgG1 reflected the acquisition of an improved resistant phenotype in HisAK70-SAL DCs vaccinated mice compared to control mice. Our results suggest that HisAK70-SAL DCs could be a promising alternative approach for vaccine delivery that has the potential to fight Leishmania infantum (L. infantum) infection.- Project number: 90
Item Elaboración del podcast “cápsulas de CINVet” (Cápsulas de Inmunoinfectología Veterinaria) para estudiantes del Grado en Veterinaria() Carrión Herrero, Francisco Javier; Jiménez Martínez, María De Los Ángeles; Blanco Gutiérrez, María Del Mar; Navarro Lozano, Vanesa; Camacho Pulido, Irene; Campos Gil, Sandra; Cascón Hernández, Ignacio; Elvira Toledano, Andrea; Jiménez Gascueña, Celia; Marín Casero, Daniel; Martín Martí, Laura; Ruiz Moñibas, Sara; López Sorribes, Elisa