Person: Blanco Gutiérrez, María Del Mar
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First Name
María Del Mar
Last Name
Blanco Gutiérrez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
10 results
Search Results
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Item 16S-23S rRNA Internal Transcribed Spacer Region (ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri(Microorganisms, 2023) Stoppani, Nadia; Colussi, Silvia; Pastorino, Paolo; Prearo, Marino; Sciuto, Simona; Altinok, Ilhan; Çağrı Öztürk, Rafet; Ture, Mustafa; Vela Alonso, Ana Isabel; Blanco Gutiérrez, María Del Mar; Kotzamanidis, Charalampos; Bitchava, Konstantina; Malousi, Andigoni; Fariano, Lucio; Volpatti, Donatella; Acutis, Pier Luigi; Fernández-Garayzábal Fernández, José FranciscoLactococcus garvieae is the etiological agent of lactococcosis, a clinically and economically significant infectious disease affecting farmed rainbow trout. L. garvieae had been considered the only cause of lactococcosis for a long time; however, L. petauri, another species of the genus Lactococcus, has lately been linked to the same disease. The genomes and biochemical profiles of L. petauri and L. garvieae have a high degree of similarity. Traditional diagnostic tests currently available cannot distinguish between these two species. The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate L. garvieae from L. petauri, saving time and money compared to genomics methods currently used as diagnostic tools for accurate discrimination between these two species. The ITS region of 82 strains was amplified and sequenced. The amplified fragments varied in size from 500 to 550 bp. Based on the sequence, seven SNPs were identified that separate L. garvieae from L. petauri. The 16S-23S rRNA ITS region has enough resolution to distinguish between closely related L. garvieae and L. petauri and it can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak.Item Utilization of lactose and presence of the phospho-β-galactosidase (lacG) gene in Lactococcus garvieae isolates from different sources(International microbiology: the official journal of the Spanish Society for Microbiology, 2010) Aguado Urda, Mónica; Blanco Gutiérrez, María Del Mar; Cutuli Simón, María Teresa; Aspiroz, Carmen; Tejedor, José L; Gibello Prieto, Alicia; Fernández-Garayzábal Fernández, José FranciscoThis study evaluates the utilization of lactose (Lac) and the presence of the phospho-β-galactosidase (lacG) gene as markers for distinguishing between fish (Lac-/lacG-) and dairy isolates (Lac+/lacG+) of Lactococcus garvieae, using a panel of L. garvieae isolates from different sources. None of the fish isolates produced acid from lactose (Lac-), however Lac-/lacG- isolates were observed in pigs, cows, birds and humans. Most of the dairy isolates (77.8%) were Lac+/lacG+, but some dairy isolates did not produce acid from this sugar. Data in the present study show that the ability to metabolize lactose and the presence of the lacG gene are heterogeneously scattered among L. garvieae isolates of different sources. Therefore, the use of these criteria as markers to differentiate between L. garvieae isolates of dairy and fish origin should be considered with caution.Item Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas(2019) Castro, R; Coll, J; Jouneau, L; Blanco Gutiérrez, María Del Mar; Rodríguez Bertos, Antonio Manuel; Fernández-Garayzábal Fernández, José Francisco; Gibello Prieto, AliciaLactococcus garvieae is a significant pathogen in aquaculture with a potential zoonotic risk. To begin to characterize the late immune response of trout to lactococcosis, we selected infected individuals showing clinical signs of lactococcosis. At the time lactococcosis clinical signs appeared, infection by L. garvieae induced a robust inflammatory response in the spleen of rainbow trout, which correlated with abundant granulomatous lesions. The response in kidney goes in parallel with that of spleen, and most of the gene regulations are similar in both organs. A correlation existed between the early inflammatory granulomas in spleen (containing macrophages with internalized L. garvieae) and up-regulated gene sets, which defined the presence of macrophages and neutrophils. This is the first analysis of the immune transcriptome of rainbow trout following L. garvieae infection during the initiation of adaptive immune mechanisms and shows a ranscriptome induction of antibody response by both IgM (+) and IgT (+) spleen B cells to respond to systemic infection. These results increase our understanding of lactococcosis and pave the way for future research to improve control measures of lactococcosis on fish farms.Item Global transcriptome analysis of Lactococcus garvieae strains in response to temperature(PloS one, 2013) Aguado Urda, Mónica; Gibello Prieto, Alicia; Blanco Gutiérrez, María Del Mar; López Alonso, Victoria; López Campos, Guillermo H; Fernández-Garayzábal Fernández, José FranciscoLactococcus garvieae is an important fish and an opportunistic human pathogen. The genomic sequences of several L. garvieae strains have been recently published, opening the possibility of global studies on the biology of this pathogen. In this study, a whole genome DNA microarray of two strains of L. garvieae was designed and validated. This DNA microarray was used to investigate the effects of growth temperature (18°C and 37°C) on the transcriptome of two clinical strains of L. garvieae that were isolated from fish (Lg8831) and from a human case of septicemia (Lg21881). The transcriptome profiles evidenced a strain-specific response to temperature, which was more evident at 18°C. Among the most significant findings, Lg8831 was found to up-regulate at 18°C several genes encoding different cold-shock and cold-induced proteins involved in an efficient adaptive response of this strain to low-temperature conditions. Another relevant result was the description, for the first time, of respiratory metabolism in L. garvieae, whose gene expression regulation was temperature-dependent in Lg21881. This study provides new insights about how environmental factors such as temperature can affect L. garvieae gene expression. These data could improve our understanding of the regulatory networks and adaptive biology of this important pathogen.Item The association of Lactococcus petauri with lactococcosis is older than expected(Aquaculture, 2023) Vela Alonso, Ana Isabel; Blanco Gutiérrez, María Del Mar; Colussi, Silvia; Charalampos, Kotzamanidis; Prearo, Marino; Altinok, Ilhan; Luigi Acutis, Pier; Volpatti, Donatella; Alba, Patricia; Feltrin, Fabiola; Ianzano, Angela; Domínguez Rodríguez, Lucas José; Fernández-Garayzábal Fernández, José FranciscoLactococcosis is a globally prevalent infectious disease that has a significant economic and sanitary impact on the rainbow trout industry. Lactococcus garvieae has traditionally been considered the only species implicated in the etiology of this disease, but Lactococcus petauri, a new species, has recently been implicated as another etiological agent. Both species cannot be distinguished by routine methods commonly used in diagnostic laboratories, resulting in their misidentification. In the present study, the identification of 48 isolates initially identified as L. garvieae was studied by determining their in-silico DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values using pairwise comparisons of their whole genome sequences and the genomes of the type strains of L. garvieae and L. petauri. The genome sequences of 37 isolates from countries in which lactococcosis can be considered endemic (Spain, Italy, Türkiye, and Greece) were obtained in this study, and the genomes of 11 isolates were retrieved from the GenBank database. Isolates from Italy, Singapore, Japan, South Korea, India, one Turkish isolate from 2013 and two Spanish isolates recovered in 1992 and 1996 were confirmed as L. garvieae. The remaining isolates from Spain and Türkiye, as well as those from Portugal, Israel, USA, and Greece were identified as L. petauri. Overall, 60.4% of isolates previously identified as L. garvieae were found to be L. petauri. These results confirm the implication of both species in the etiology of lactococcosis and suggest that L. petauri plays a significant role in the epidemiology of this disease. Some of the isolates identified as L. petauri in the present study were isolated three decades ago, indicating that its association with lactococcosis is older than might be expected from the recent descriptions. The commercial Rapid ID32 Strep system was unable to discriminate between L. garvieae and L. petauri. However, both species exhibited some biochemical differences that might serve as phenotypic markers for their presumptive recognition. Consequently, isolates that hydrolyze hippurate and produce acid from sucrose and tagatose could be presumptively recognized as L. petauri, while those that fail these tests could be identified as L. garvieae. The results of this work indicate that great attention should be given to L. petauri in the epidemiology of lactococcosis.- Project number: nº 136
Item La innovación educativa aplicada a la Ictiopatología(2018) Vela Alonso, Ana Isabel; Blanco Gutiérrez, María Del Mar; Gibello Prieto, Alicia; Cutuli Simón, María Teresa; Sánchez-Fortún Rodríguez, Sebastián; Ruiz Zarzuela, Imanol; Blas Giral, Ignacio de; Muniesa del Campo, Ana; Fernández-Garayzábal Fernández, José FranciscoSe ha elaborado un material aplicable a la docencia y que puede favorecer el ejercicio profesional de un veterinario y de otras áreas dedicados a la Acuicultura. Además, tiene el valor añadido de ser una colaboración entre docentes pertenecientes a dos Universidades. Item Laboratorio virtual de microbiología veterinaria(III jornada Campus Virtual UCM : Innovación en el Campus Virtual metodologías y herramientas, 2007) Gómez-Lucía Duato, María Esperanza; Gibello Prieto, Alicia; Cutuli Simón, María Teresa; Aranaz Martín, Alicia; Blanco Gutiérrez, María Del Mar; Blanco Cancelo, José Luis; García Sánchez, Marta Eulalia; González Zorn, Bruno; Fernández-Garayzábal Fernández, José Francisco; Fernández-Valmayor Crespo, Alfredo; Fernández-Pampillón Cesteros, Ana María; Merino Granizo, JorgePara el Proyecto de Innovación Educativa se ha realizado un CD como complemento a las prácticas de la Licenciatura de Veterinaria que tienen un componente microbiológico. Incluye aspectos fundamentales del ejercicio profesional (Toma de Muestras, Bioseguridad, Laboratorio de Virología), pero que con las infraestructuras actuales es casi imposible presentarlos al alumno de forma eficaz y personalizada. Para su desarrollo hemos elaborado el material escrito, apoyándolo con imágenes y videos explicativos. La confección del CD en sí ha sido realizada por una empresa de informática, siguiendo nuestras especificaciones y requerimientos.Item Characterization of plasmids in a human clinical strain of Lactococcus garvieae(PLoS ONE, 2012) Aguado Urda, Mónica; Gibello Prieto, Alicia; Blanco Gutiérrez, María Del Mar; López Campos, Guillermo H; Cutuli Simón, María Teresa; Fernández-Garayzábal Fernández, José FranciscoThe present work describes the molecular characterization of five circular plasmids found in the human clinical strain Lactococcus garvieae 21881. The plasmids were designated pGL1-pGL5, with molecular sizes of 4,536 bp, 4,572 bp, 12,948 bp, 14,006 bp and 68,798 bp, respectively. Based on detailed sequence analysis, some of these plasmids appear to be mosaics composed of DNA obtained by modular exchange between different species of lactic acid bacteria. Based on sequence data and the derived presence of certain genes and proteins, the plasmid pGL2 appears to replicate via a rolling-circle mechanism, while the other four plasmids appear to belong to the group of lactococcal theta-type replicons. The plasmids pGL1, pGL2 and pGL5 encode putative proteins related with bacteriocin synthesis and bacteriocin secretion and immunity. The plasmid pGL5 harbors genes (txn, orf5 and orf25) encoding proteins that could be considered putative virulence factors. The gene txn encodes a protein with an enzymatic domain corresponding to the family actin-ADP-ribosyltransferases toxins, which are known to play a key role in pathogenesis of a variety of bacterial pathogens. The genes orf5 and orf25 encode two putative surface proteins containing the cell wall-sorting motif LPXTG, with mucin-binding and collagen-binding protein domains, respectively. These proteins could be involved in the adherence of L. garvieae to mucus from the intestine, facilitating further interaction with intestinal epithelial cells and to collagenous tissues such as the collagen-rich heart valves. To our knowledge, this is the first report on the characterization of plasmids in a human clinical strain of this pathogen.Item Genome sequence of Lactococcus garvieae 21881, isolated in a case of human septicemia(Journal of bacteriology, 2011) Aguado Urda, Mónica; López Campos, Guillermo H; Blanco Gutiérrez, María Del Mar; Cutuli Simón, María Teresa; Aspiroz, Carmen; López Alonso, Victoria; Gibello Prieto, Alicia; Fernández-Garayzábal Fernández, José FranciscoLactococcus garvieae is a Gram-positive bacterium considered an important opportunistic emerging human pathogen and also a well-recognized fish pathogen. Here, we present the draft genome sequence of Lactococcus garvieae strain 21881 (2,164,557 bp, with a G+C content of 37.9%), which represents the first report of a genome sequence on Lactococcus garvieae.Item Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization(BMC microbiology, 2010) Aguado Urda, Mónica; López Campos, Guillermo H; Martín Sánchez, Fernando; Gibello Prieto, Alicia; Blanco Gutiérrez, María Del Mar; Domínguez Rodríguez, Lucas José; Fernández-Garayzábal Fernández, José FranciscoBACKGROUND Lactococcus garvieae is a bacterial pathogen that affects different animal species in addition to humans. Despite the widespread distribution and emerging clinical significance of L. garvieae in both veterinary and human medicine, there is almost a complete lack of knowledge about the genetic content of this microorganism. In the present study, the genomic content of L. garvieae CECT 4531 was analysed using bioinformatics tools and microarray-based comparative genomic hybridization (CGH) experiments. Lactococcus lactis subsp. lactis IL1403 and Streptococcus pneumoniae TIGR4 were used as reference microorganisms. RESULTS The combination and integration of in silico analyses and in vitro CGH experiments, performed in comparison with the reference microorganisms, allowed establishment of an inter-species hybridization framework with a detection threshold based on a sequence similarity of >or= 70%. With this threshold value, 267 genes were identified as having an analogue in L. garvieae, most of which (n = 258) have been documented for the first time in this pathogen. Most of the genes are related to ribosomal, sugar metabolism or energy conversion systems. Some of the identified genes, such as als and mycA, could be involved in the pathogenesis of L. garvieae infections. CONCLUSIONS In this study, we identified 267 genes that were potentially present in L. garvieae CECT 4531. Some of the identified genes could be involved in the pathogenesis of L. garvieae infections. These results provide the first insight into the genome content of L. garvieae.