Person: Blanco Gutiérrez, María Del Mar
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First Name
María Del Mar
Last Name
Blanco Gutiérrez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
4 results
Search Results
Now showing 1 - 4 of 4
Item 16S-23S rRNA Internal Transcribed Spacer Region (ITS) Sequencing: A Potential Molecular Diagnostic Tool for Differentiating Lactococcus garvieae and Lactococcus petauri(Microorganisms, 2023) Stoppani, Nadia; Colussi, Silvia; Pastorino, Paolo; Prearo, Marino; Sciuto, Simona; Altinok, Ilhan; Çağrı Öztürk, Rafet; Ture, Mustafa; Vela Alonso, Ana Isabel; Blanco Gutiérrez, María Del Mar; Kotzamanidis, Charalampos; Bitchava, Konstantina; Malousi, Andigoni; Fariano, Lucio; Volpatti, Donatella; Acutis, Pier Luigi; Fernández-Garayzábal Fernández, José FranciscoLactococcus garvieae is the etiological agent of lactococcosis, a clinically and economically significant infectious disease affecting farmed rainbow trout. L. garvieae had been considered the only cause of lactococcosis for a long time; however, L. petauri, another species of the genus Lactococcus, has lately been linked to the same disease. The genomes and biochemical profiles of L. petauri and L. garvieae have a high degree of similarity. Traditional diagnostic tests currently available cannot distinguish between these two species. The aim of this study was to use the transcribed spacer (ITS) region between 16S rRNA and 23S rRNA as a potential useful molecular target to differentiate L. garvieae from L. petauri, saving time and money compared to genomics methods currently used as diagnostic tools for accurate discrimination between these two species. The ITS region of 82 strains was amplified and sequenced. The amplified fragments varied in size from 500 to 550 bp. Based on the sequence, seven SNPs were identified that separate L. garvieae from L. petauri. The 16S-23S rRNA ITS region has enough resolution to distinguish between closely related L. garvieae and L. petauri and it can be used as a diagnostic marker to quickly identify the pathogens in a lactococcosis outbreak.Item The association of Lactococcus petauri with lactococcosis is older than expected(Aquaculture, 2023) Vela Alonso, Ana Isabel; Blanco Gutiérrez, María Del Mar; Colussi, Silvia; Charalampos, Kotzamanidis; Prearo, Marino; Altinok, Ilhan; Luigi Acutis, Pier; Volpatti, Donatella; Alba, Patricia; Feltrin, Fabiola; Ianzano, Angela; Domínguez Rodríguez, Lucas José; Fernández-Garayzábal Fernández, José FranciscoLactococcosis is a globally prevalent infectious disease that has a significant economic and sanitary impact on the rainbow trout industry. Lactococcus garvieae has traditionally been considered the only species implicated in the etiology of this disease, but Lactococcus petauri, a new species, has recently been implicated as another etiological agent. Both species cannot be distinguished by routine methods commonly used in diagnostic laboratories, resulting in their misidentification. In the present study, the identification of 48 isolates initially identified as L. garvieae was studied by determining their in-silico DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values using pairwise comparisons of their whole genome sequences and the genomes of the type strains of L. garvieae and L. petauri. The genome sequences of 37 isolates from countries in which lactococcosis can be considered endemic (Spain, Italy, Türkiye, and Greece) were obtained in this study, and the genomes of 11 isolates were retrieved from the GenBank database. Isolates from Italy, Singapore, Japan, South Korea, India, one Turkish isolate from 2013 and two Spanish isolates recovered in 1992 and 1996 were confirmed as L. garvieae. The remaining isolates from Spain and Türkiye, as well as those from Portugal, Israel, USA, and Greece were identified as L. petauri. Overall, 60.4% of isolates previously identified as L. garvieae were found to be L. petauri. These results confirm the implication of both species in the etiology of lactococcosis and suggest that L. petauri plays a significant role in the epidemiology of this disease. Some of the isolates identified as L. petauri in the present study were isolated three decades ago, indicating that its association with lactococcosis is older than might be expected from the recent descriptions. The commercial Rapid ID32 Strep system was unable to discriminate between L. garvieae and L. petauri. However, both species exhibited some biochemical differences that might serve as phenotypic markers for their presumptive recognition. Consequently, isolates that hydrolyze hippurate and produce acid from sucrose and tagatose could be presumptively recognized as L. petauri, while those that fail these tests could be identified as L. garvieae. The results of this work indicate that great attention should be given to L. petauri in the epidemiology of lactococcosis.- Project number: 175
Item Virología en acción: aprender-practicando sobre diagnóstico virológico(2022) Domenech Gómez, Ana María; Benitez Rico, Laura; Blanco Gutiérrez, María Del Mar; Bonel Ayuso, Diego Paul; Cobos Blázquez, Sofía; Fandiño González, Sergio; García Cabrera, José Antonio; Gavilán García, María Reyes; Gómez-Lucía Duato, María Esperanza; Madrid González, Ricardo; Tolosa, Rocío MirandaVirología en acción es una iniciativa financiada por los Proyectos de Innova-Docencia UCM 2021-22 (nº 175) que ha desarrollado una herramienta interactiva online para mejorar la docencia de la Virología. El objetivo es aprender a “saber hacer” más que a memorizar contenidos en virología, completando la formación teórica y práctica que reciben los estudiantes en esta materia, en especial en la interpretación de casos prácticos y de resultados del diagnóstico virológico. - Project number: 171
Item Detectives de alimentos: un trabajo en equipo para la investigación de toxiinfecciones alimentarias(2021) Herranz Sorribes, Carmen; Haza Duaso, Ana Isabel; Rodríguez Gómez, Juan Miguel; Morales Gómez, Paloma; Domínguez Bernal, Gustavo Ramón; Alfonso Álvarez, Virginia; Aller Galán, Patricia; Arribas Gómez, María; Blanco Gutiérrez, María Del Mar; Chacha Hidalgo, María Cristina; Checa Martín, Gemma; Dschoutezo Gordo, Sophie Marie; Escobar Pastor, María José; Fernández Cano, Clara; Fernández Zambrano, Lucía; Gabriel Peg, Patricia de; Gardel Ron, Paula; Garzás Sánchez, Sara María; Gattoni Krüger, Paula Verena; González Tejero, Silvia; Pascual Ocón, Carmen; Peñil Martín-Serrano, Raquel; Pérez Cepas, Carmen; Rayo Juárez, Claudia; Rupérez García, Alicia; Sanz Almaraz, LauraProyecto de Innovación Docente en el que los estudiantes realizan un trabajo cooperativo mediante el método del puzle para diseñar fichas descriptivas de patógenos de transmisión alimentaria y resolver un caso de toxiinfección alimentaria