Person: Fontanillas Pérez, Juan Carlos
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First Name
Juan Carlos
Last Name
Fontanillas Pérez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Fisiología
Area
Zoología
Identifiers
4 results
Search Results
Now showing 1 - 4 of 4
Item Light Microscopic, Ultrastructure Analysis and Functional Morphology of Cornu aspersum Spermatozoa Containedin the Frozen Hermaphroditic Duct(Int. J. Morphol, 2023) Fontanillas Pérez, Juan Carlos; Fuertes Recuero, Manuel; Rojo Salvador, Concepción; García-Cuenca Ariati, IsabelIn this study we describe the functional morphology of Cornu aspersum (Helix aspersa), spermatozoa using light, scanning (SEM) and transmission electron (TEM) microscopies. The studies were performed with sperm located in the frozen hermaphroditic duct. Our results showed that the head presents an elongated conical shape slightly coiled in a corkscrew, with the nucleus partially covered by an acrosome, where an apical vesicle is located at the lateralized apex. This peculiar shape suggests the helical displacement movement of the spermatozoa. The head and the nucleus are slightly larger size compared to those of other gastropod species. The intermediate tract is surrounded by a mitochondrial complex and a glycogen helix. The glycogen helix is coiled helically along the intermediate tract, presenting at least five twists of glycogen helices. The complexity of both the mitochondrial complex and the glycogen helix suggests a high metabolic consumption considering the long period of time until fertilization occurs. Our findings on the detailed characterization of Cornu aspersum spermatozoa, obtained from a frozen hermaphroditic duct can contribute to a better understanding of the functional morphology of sperm and serve as a reference for future studies.Item First report of polymelia in a passerine bird, the Pied Flycatcher(Ardea, 2023) Fontanillas Pérez, Juan Carlos; Cantarero Carmona, Alejandro; Fuertes Recuero, Manuel; Longhin, Davide; Chiesurin, Leonardo; Tusini, SamuelePolymelia is a congenital defect characterized by an excessive number of limbs. Leg malformations are unusual and have rarely been reported in birds. The ultimate mechanism that regulates this type of abnormal development is not yet well understood. Here we report polymelia in a nestling of the European Pied Flycatcher Ficedula hypoleuca. We have ringed more than 12,100 Pied Flycatcher nestlings since 1991 and have never observed this malformation before. To our knowledge, there is no study indicating polymelia in any bird of the order Passeriformes. We observed and studied a Pied Flycatcher nestling that had two extra limbs fused at the pelvis. We used X-rays, Computerized Tomography (CT) scan and Magnetic Resonance Imaging (MRI). We describe the observed malformations and discuss potential causes. The malformation could be due to an embryonic cause, such as a parasitic twin (pygopagus parasitic) or a genetic mutation.Item Determination of the appropriate concentration of sodium alginate used for in vitro culture of cat preantral follicles in a serum-free medium containing FSH, EGF and IGF-I(Reproduction in Domestic Animals, 2023) Fontanillas Pérez, Juan Carlos; González Gil, Alfredo; García-Cuenca Ariati, Isabel; Picazo González, María Dolores; Fuertes Recuero, ManuelCulture of domestic cat preantral follicles can be a suitable technology to assist oocyte conservation strategies in the family Felidae. This research was aimed to comparatively analyse cat preantral follicular development of follicles directly seeded on growth surface or encapsulated in 0.5 or 1% of sodium alginate in a serum-free medium containing FSH, EGF and IGF-I. Preantral follicles were isolated from cat ovarian cortical tissue after ovariectomy. Alginate was dissolved at 0.5 or 1% in PBS. Follicles, 4 per well, with 0% (G-0%), 0.5% (G-0.5%) or 1% (G-1%) of sodium alginate were cultured in M199 with FSH (100 ng/mL), EGF (100 ng/mL) and IGF-I (100 ng/mL) for 7 days at 37°C, 5% CO2 and 99% humidity. Culture medium was replaced every 48 h and samples were stored at −20°C until ELISA of steroid hormones. Morphometric evaluation of follicles was performed every 24 h. G-0% follicles showed granulosa cell migration away from the oocyte and disrupted morphology, whereby they reached apparently larger diameters (203.70 ± 5.82 μm; p < .05) than G-0.5% and G-1% follicles (157.89 ± 8.47 μm and 95.23 ± 1.67 μm, respectively) which maintained three-dimensional organization, being larger in G-0.5% than in G-1% (p < .05). G-0.5% follicles attained the multi-layer preantral follicle stage on day 7 of culture, whereas G-1% follicles underwent progressive atresia. On day 6, steroid concentrations were higher (p < .05) in G-0% than in G-1%: 60 ± 19 vs 0.88 ± 0.32 pg/mL oestradiol; 2.6 ± 0.84 vs 0.04 ± 0.02 ng/mL progesterone; 1.3 ± 0.22 vs 0.61 ± 0.04 ng/mL testosterone and 1.6 ± 0.54 vs 0.22 ± 0.07 ng/mL androstenedione respectively. Steroid concentrations in G-0.5% were comprised between those of G-0% and G-1% (p > .05). In conclusion, two-layer cat preantral follicles encapsulated in 0.5% alginate cultured in medium containing FSH, EGF and IGF-I can develop up to the multi-layer preantral stage in 7 days of culture, whereas follicles directly seeded on growth surface or encapsulated in 1% alginate lost their three-dimensional organization, and experienced regression with compromised steroidogenesis, respectively.Item Postoperative pain in dogs undergoing either laparoscopic or open ovariectomy(The Veterinary Journal, 2024) Fuertes Recuero, Manuel; Álvarez Gómez De Segura, Ignacio; Sánchez López, A.; Suárez Redondo, María; Canfrán Arrabe, Susana; Penelo Hidalgo, Silvia; Fontanillas Pérez, Juan Carlos; Ortiz Díez, GustavoA prospective, quasi-experimental, clinical trial was performed to assess acute postoperative pain in healthy female dogs following elective ovariectomy by either laparoscopy (n=13) or laparotomy (n=14). Pain was assessed by both a veterinarian at the hospital, and by the owner once the patient was discharged. The Spanish version of the short form of the Glasgow Composite Measuring Pain Scale (CMPS-SF) was used. Pain scores were assessed by the veterinarian preoperatively and at 1, 2, 4, and 6 h after extubation, whilst owner-assessed scores were performed preoperatively and at postoperative days 0, 1, 2, 3, 5 and 7. Data were compared with Mann-Whitney-U test. Veterinarian-assessed CMPS-SF scores were different between both groups at all postoperative times but not at baseline, being below 6/24 in all dogs in the laparoscopy group, but equal to or greater than 6/24 in the laparotomy group at 1 h (n=12), and 4 h (n=4) (P<0.001 and P=0.029, respectively). There were also differences in pain scores between both groups at 2 h (P=0.012) and 6 h (P=0.007), being below 6/24 in all of them. However, there were no differences in owner assessments between groups. In conclusion, ovariectomy performed by laparoscopy induced lower pain scores that were below the pain threshold set by the CMPS-SF during the first 6 h postoperatively. After discharge, and up to one week later, ongoing owner-assessed scores suggest no pain was induced with neither of the techniques. Owners were proactive allowing real-time pain assessment to be reported. The development and validation of instruments for acute pain assessment by owners is warranted, as these tools are currently lacking.