Rivera Arroyo, Belén

Profile Picture
First Name
Last Name
Rivera Arroyo
Universidad Complutense de Madrid
Faculty / Institute
UCM identifierScopus Author IDDialnet ID

Search Results

Now showing 1 - 6 of 6
  • Publication
    Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
    (MDPI, 2020-02-28) Cadenas Fernández, Estefanía; Sánchez-Vizcaíno, José Manuel; Kosowska, Aleksandra; Rivera Arroyo, Belén; Mayoral Alegre, Francisco José; Rodríguez Bertos, Antonio Manuel; Yao, Jianxiu; Bray, Jocelyn; Lokhandwala, Shehnaz; Mwangi, Waithaka; Barasona García-Arévalo, José Ángel
    African swine fever (ASF) is a viral disease of domestic and wild suids for which there is currently no vaccine or treatment available. The recent spread of ASF virus (ASFV) through Europe and Asia is causing enormous economic and animal losses. Unfortunately, the measures taken so far are insufficient and an effective vaccine against ASFV needs to be urgently developed. We hypothesized that immunization with a cocktail of thirty-five rationally selected antigens would improve the protective efficacy of subunit vaccine prototypes given that the combination of fewer immunogenic antigens (between 2 and 22) has failed to elicit protective efficacy. To this end, immunogenicity and efficacy of thirty-five adenovirus-vectored ASFV antigens were evaluated in wild boar. The treated animals were divided into different groups to test the use of BioMize adjuvant and different inoculation strategies. Forty-eight days after priming, the nine treated and two control wild boar were challenged with the virulent ASFV Arm07 isolate. All animals showed clinical signs and pathological findings consistent with ASF. This lack of protection is in line with other studies with subunit vaccine prototypes, demonstrating that there is still much room for improvement to obtain an effective subunit ASFV vaccine.
  • Publication
    Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar
    (MDPI, 2020-03-25) Barasona García-Arévalo, José Ángel; Barroso Arévalo, Sandra; Rivera Arroyo, Belén; Gortázar, Christian; Sánchez-Vizcaíno, José Manuel
    The presence of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex (MTC) is a main concern in wildlife populations such as the Eurasian wild boar (Sus scrofa). Tests detecting antibodies against the MTC are valuable for tuberculosis (TB) monitoring and control and particularly useful in suids. The development of accurate, efficient, and non-invasive new tools to detect exposure to MTC would be highly beneficial for improving disease surveillance. This study aimed to determine if antibodies against MTC could be detected in oral fluid (OF) samples by a new ELISA test (IgG detection) from naturally TB-infected wild boar. For this, individual, paired serum and OF samples were collected from 148 live wild boar in two TB-status areas from Spain and quantitatively used to validate the new ELISA test. Antibodies against MTC were widely detected in OF samples, for which a significant positive correlation (r = 0.83) was found with the validated serology test. OF ELISA sensitivity and specificity were 67.3% and 100%, respectively. The results of this work suggest that OF samples have the potential to be used for MTC diagnosis as a further step in TB surveillance and control in suid populations. Based on our results, further research is warranted and could be performed using non-invasive new tools directly in field conditions to detect exposure to MTC.
  • Publication
    Evidence of shared bovine viral diarrhea infections between red deer and extensively raised cattle in south-central Spain
    (2016) Rodríguez Prieto, Víctor; Kukielka, Deborah; Rivera Arroyo, Belén; Martínez López, Beatriz; de las Heras, Ana Isabel; Sánchez-Vizcaíno, José Manuel; Vicente, Joaquín
    Bovine viral diarrhea virus (BVDV) is a pestivirus that affects cattle production worldwide and that can infect other ungulates such as cervids and even wild boar (Sus scrofa). It is believed that domestic livestock can become infected through contact with wild animals, though it is known that infection can spread among wild animals in the absence of contact with livestock. Little is known about the sharing of BVDV infection between wild and domestic animals in the same habitat, which is important for designing eradication campaigns and preventing outbreaks, especially on hunting estates with high animal densities.
  • Publication
    Evaluation of the clinical evolution and transmission of SARS-CoV-2 infection in cats by simulating natural routes of infection
    (Springer Nature, 2022-03-03) Barroso Arévalo, Sandra; Sánchez Morales, Lidia; Barasona García-Arévalo, José Ángel; Rivera Arroyo, Belén; Sánchez García, Rocío; Risalde, María A.; Agulló-Ros, Irene; Sánchez Vizcaíno, José Manuel
    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the current pandemic disease denominated as Coronavirus Disease 2019 (COVID-19). Several studies suggest that the original source of this virus was a spillover from an animal reservoir and its subsequent adaptation to humans. Of all the different animals affected, cats are one of the most susceptible species. Moreover, several cases of natural infection in domestic and stray cats have been reported in the last few months. Although experimental infection assays have demonstrated that cats are successfully infected and can transmit the virus to other cats by aerosol, the conditions used for these experiments have not been specified in terms of ventilation. We have, therefore, evaluated the susceptibility of cats using routes of infection similar to those expected under natural conditions (exposure to a sneeze, cough, or contaminated environment) by aerosol and oral infection. We have also evaluated the transmission capacity among infected and naïve cats using different air exchange levels. Despite being infected using natural routes and shed virus for a long period, the cats did not transmit the virus to contact cats when air renovation features were employed. The infected animals also developed gross and histological lesions in several organs. These outcomes confirm that cats are at risk of infection when exposed to infected people, but do not transmit the virus to other cats with high rates of air renovation.
  • Publication
    First molecular detection and characterization of herpesvirus and poxvirus in a Pacific walrus (Odobenus rosmarus divergens)
    (BioMedCentral, 2014-12-21) Melero Asensio, Mar; García Párraga, Daniel; Corpa, Juan Manuel; Ortega, Joaquín; Rubio Guerri, Consuelo; Crespo, José Luis; Rivera Arroyo, Belén; Sánchez-Vizcaíno, José Manuel
    BACKGROUND Herpesvirus and poxvirus can infect a wide range of species: herpesvirus genetic material has been detected and amplified in five species of the superfamily Pinnipedia; poxvirus genetic material, in eight species of Pinnipedia. To date, however, genetic material of these viruses has not been detected in walrus (Odobenus rosmarus), another marine mammal of the Pinnipedia clade, even though anti-herpesvirus antibodies have been detected in these animals. CASE PRESENTATION In February 2013, a 9-year-old healthy captive female Pacific walrus died unexpectedly at L'Oceanografic (Valencia, Spain). Herpesvirus was detected in pharyngeal tonsil tissue by PCR. Phylogenetic analysis revealed that the virus belongs to the subfamily Gammaherpesvirinae. Poxvirus was also detected by PCR in skin, pre-scapular and tracheobronchial lymph nodes and tonsils. Gross lesions were not detected in any tissue, but histopathological analyses of pharyngeal tonsils and lymph nodes revealed remarkable lymphoid depletion and lymphocytolysis. Similar histopathological lesions have been previously described in bovine calves infected with an alphaherpesvirus, and in northern elephant seals infected with a gammaherpesvirus that is closely related to the herpesvirus found in this case. Intracytoplasmic eosinophilic inclusion bodies, consistent with poxviral infection, were also observed in the epithelium of the tonsilar mucosa. CONCLUSION To our knowledge, this is the first molecular identification of herpesvirus and poxvirus in a walrus. Neither virus was likely to have contributed directly to the death of our animal.
  • Publication
    Large-scale study on virological and serological prevalence of SARS-CoV-2 in cats and dogs in Spain
    (Wiley, 2022-07) Barroso Arévalo, Sandra; Barneto, Alberto; Ramos, Angel M; Rivera Arroyo, Belén; Sánchez García, Rocío; Sánchez Morales, Lidia; Perez Sancho, Marta; Buendía Andrés, Aranzazu; Ferreras, Elisa; Ortiz Menéndez, Juan Carlos; Moreno, Inmaculada; Serres Dalmau, Consuelo; Vela, Carmen; Risalde, María A.; Domínguez Rodríguez, Lucas; Sánchez Vizcaíno, José Manuel
    The disease produced by the severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) is currently one of the primary concerns worldwide. Knowing the zoonotic origin of the disease and that several animal species, including dogs and cats, are susceptible to viral infection, it is critical to assess the relevance of pets in this pandemic. Here, we performed a large-scale study on SARS-CoV-2 serological and viral prevalence in cats and dogs in Spain in order to elucidate their role and susceptibility. Samples from animals in contact with COVID-19 positive people and/or compatible symptoms (n = 492), as well as from random animals (n = 1024), were taken. Despite the large number of animals analyzed, only 12 animals (eight dogs and four cats), which represents 0.79% of the total analyzed animals (n = 1516), were positive for viral SARS-CoV-2 RNA detection by reverse transcription quantitative PCR (RT-qPCR) in which viral isolation was possible in four animals. We detected neutralizing antibodies in 34 animals, four of them were also positive for PCR. This study evidences that pets are susceptible to SARS-CoV-2 infection in natural conditions but at a low level, as evidenced by the low percentage of positive animals detected, being infected humans the main source of infection. However, the inclusion of animals in the surveillance of COVID-19 is still recommended.