Person: Luque García, José Luis
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First Name
José Luis
Last Name
Luque García
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
Identifiers
6 results
Search Results
Now showing 1 - 6 of 6
- PublicationHerpesvirus Antibodies, Vitamin D and Short-Chain Fatty Acids: Their Correlation with Cell Subsets in Multiple Sclerosis Patients and Healthy Controls(MPDI, 2021-01-10) Domínguez Mozo, María Inmaculada; Pérez Pérez, Silvia; Villarrubia, Noelia; Costa Frossard, Lucienne; Fernández Velasco, Jose Ignacio; Ortega Madueño, Isabel; García Martínez, María Angel; García Calvo, Estefanía; Estévez Sánchez, Héctor; Luque García, José Luis; Torrejón, Maria Josefa; Arroyo, Rafael; Villar, Luisa Maria; Álvarez Lafuente, RobertoAlthough the etiology of multiple sclerosis (MS) is still unknown, it is commonly accepted that environmental factors could contribute to the disease. The objective of this study was to analyze the humoral response to Epstein-Barr virus, human herpesvirus 6A/B and cytomegalovirus, and the levels of 25-hydroxyvitamin D (25(OH)D) and the three main short-chain fatty acids (SCFA), propionate (PA), butyrate (BA) and acetate (AA), in MS patients and healthy controls (HC) to understand how they could contribute to the pathogenesis of the disease. With this purpose, we analyzed the correlations among them and with different clinical variables and a wide panel of cell subsets. We found statistically significant differences for most of the environmental factors analyzed when we compared MS patients and HC, supporting their possible involvement in the disease. The strongest correlations with the clinical variables and the cell subsets analyzed were found for 25(OH)D and SCFAs levels. A correlation was also found between 25(OH)D and PA/AA ratio, and the interaction between these factors negatively correlated with interleukin 17 (IL-17)-producing CD4+ and CD8+ T cells in untreated MS patients. Therapies that simultaneously increase vitamin D levels and modify the proportion of SCFA could be evaluated in the future.
- PublicationIntegrated transcriptomics and metabolomics analysis reveals the biomolecular mechanisms associated to the antitumoral potential of a novel silver‑based core@shell nanosystem(Springer, 2023-03-13) Aragoneses Cazorla, Guillermo; Vallet Regí, María; Gómez Gómez, M.Milagros; González Ortiz, Blanca; Luque García, José LuisA combination of omics techniques (transcriptomics and metabolomics) has been used to elucidate the mechanisms responsible for the antitumor action of a nanosystem based on a Ag core coated with mesoporous silica on which transferrin has been anchored as a targeting ligand against tumor cells (Ag@MSNs-Tf). Transcriptomics analysis has been carried out by gene microarrays and RT-qPCR, while high-resolution mass spectrometry has been used for metabolomics. This multiomics strategy has enabled the discovery of the effect of this nanosystem on different key molecular pathways including the glycolysis, the pentose phosphate pathway, the oxidative phosphorylation and the synthesis of fatty acids, among others.
- PublicationA Multi-Omics Approach to Evaluate the Toxicity Mechanisms Associated with Silver Nanoparticles Exposure(MDPI, 2022) Aragoneses Cazorla, Guillermo; Buendia Nacarino, M.Pilar; Mena, María Luz; Luque García, José LuisSilver nanoparticles (AgNPs) are currently used in many different industrial, commercial and health fields, mainly due to their antibacterial properties. Due to this widespread use, humans and the environment are increasingly exposed to these types of nanoparticles, which is the reason why the evaluation of the potential toxicity associated with AgNPs is of great importance. Although some of the toxic effects induced by AgNPs have already been shown, the elucidation of more complete mechanisms is yet to be achieved. In this sense, and since the integration of metabolomics and transcriptomics approaches constitutes a very useful strategy, in the present study targeted and untargeted metabolomics and DNA microarrays assays have been combined to evaluate the molecular mechanisms involved in the toxicity induced by 10 nm AgNPs. The results have shown that AgNPs induce the synthesis of glutathione as a cellular defense mechanism to face the oxidative environment, while inducing the depletion of relevant molecules implicated in the synthesis of important antioxidants. In addition, it has been observed that AgNPs completely impair the intracellular energetic metabolism, especially affecting the production of adenosine triphosphate (ATP) and disrupting the tricarboxylic acids cycle. It has been demonstrated that AgNPs exposure also affects the glycolysis pathway. The effect on such pathway differs depending on the step of the cycle, which a significant increase in the levels of glucose as way to counterbalance the depleted levels of ATP.
- PublicationMesoporous silica nanoparticles containing silver as novel antimycobacterial agents against Mycobacterium tuberculosis(Elsevier, 2020-10-04) Montalvo Quirós, Sandra; Gómez Graña, Sergio; Vallet Regí, María; Prados Rosales, Rafael; González Ortiz, Blanca; Luque García, José LuisTuberculosis remains today a major public health issue with a total of 9 million new cases and 2 million deaths annually. The lack of an effective vaccine and the increasing emergence of new strains of Mycobacterium tuberculosis (Mtb) highly resistant to antibiotics, anticipate a complicated scenario in the near future. The use of nanoparticles features as an alternative to antibiotics in tackling this problem due to their potential effectiveness in resistant bacterial strains. In this context, silver nanoparticles have demonstrated high bactericidal efficacy,although their use is limited by their relatively high toxicity, which calls for the design of nanocarriers that allow silver based nanoparticles to be safely delivered to the target cells or tissues. In this work mesoporous silica nanoparticles are used as carriers of silver based nanoparticles as antimycobacterial agent against Mtb. Two different synthetic approaches have been used to afford, on the one hand, a 2D hexagonal mesoporous silica nanosystem which contains silver bromide nanoparticles distributed all through the silica network and, on the other hand, a core@shell nanosystem with metallic silver nanoparticles as core and mesoporous silica shell in a radial mesoporous rearrangement. Both materials have demonstrated good antimycobacterial capacity in in vitro test using Mtb, being lower the minimum inhibitory concentration for the nanosystem which contains silver bromide. Therefore, the interaction of this material with the mycobacterial cell has been studied by cryo-electron microscopy, establishing a direct connection between the antimycobactericidal effect observed and the damage induced in the cell envelope.
- PublicationTranscriptome Analysis Identifies Novel Mechanisms Associated with the Antitumor Effect of Chitosan‐Stabilized Selenium Nanoparticles.(MDPI, 2021-03-08) Estévez Sánchez, Héctor; García Calvo, Estefanía; Rivera-Torres, José; Vallet Regí, María; González Ortiz, Blanca; Luque García, José LuisSelenium nanoparticles (SeNPs) have been receiving special attention in recent years due to their antioxidant capacity and antitumor properties. However, the mechanisms associated with these properties remain to be elucidated. For this reason, a global transcriptome analysis has been designed in this work and it was carried out using human hepatocarcinoma cells and chitosan‐ stabilized SeNPs (Ch‐SeNPs) to identify new targets and pathways related to the antitumor mechanisms associated with Ch‐SeNPs. The results obtained confirm the alteration of the cell cycle and the effect of Ch‐SeNPs on different tumor suppressors and other molecules involved in key mechanisms related to cancer progression. Furthermore, we demonstrated the antioxidant properties of these nanoparticles and their capacity to induce senescence, which was further confirmed through the measurement of β‐galactosidase activity.
- PublicationSMARTeaching: Uso de dispositivos móviles en el aula y eficacia de las clases presenciales(2018-06-30) García Segura, Juan Manuel; Feito Castellano, María José; Lacadena García-Gallo, Javier; Lainez Ferrando, Alfredo; Luque García, José Luis; OñaderraA Sánchez, Mercedes; Ortega Gutiérrez, Silvia; Pérez-Gil, Jesús; Sánchez García, Cristina