Person: Olives Barba, Ana Isabel
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First Name
Ana Isabel
Last Name
Olives Barba
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
Química Analítica
Identifiers
5 results
Search Results
Now showing 1 - 5 of 5
- PublicationBisavenathramide Analogues as Nrf2 Inductors and Neuroprotectors in In Vitro Models of Oxidative Stress and Hyperphosphorylation(MDPI, 2021-06-10) Cores Esperón, Ángel; Abril, Sheila; Michalska, Patrycja; Duarte, Pablo; Olives Barba, Ana Isabel; Martín Carmona, María Antonia; Villacampa Sanz, Mercedes; León, Rafael; Menéndez Ramos, José CarlosOxidative stress is crucial to the outbreak and advancement of neurodegenerative diseases and is a common factor to many of them. We describe the synthesis of a library of derivatives of the 4-arylmethylen-2-pyrrolin-5-one framework by sequential application of a three-component reaction of primary amines, β-dicarbonyl compounds, and α-haloketones and a Knoevenagel condensation. These compounds can be viewed as cyclic amides of caffeic and ferulic acids, and are also structurally related to the bisavenanthramide family of natural antioxidants. Most members of the library showed low cytotoxicity and good activity as inductors of Nrf2, a transcription factor that acts as the master regulator of the antioxidant response associated with activation of the antioxidant response element (ARE). Nrf2-dependent protein expression was also proved by the significant increase in the levels of the HMOX1 and NQO1 proteins. Some compounds exerted neuroprotective properties in oxidative stress situations, such as rotenone/oligomycin-induced toxicity, and also against protein hyperphosphorylation induced by the phosphatase inhibitor okadaic acid. Compound 3i, which can be considered a good candidate for further hit-to-lead development against neurodegenerative diseases due to its well-balanced multitarget profile, was further characterized by proving its ability to reduce phosphorylated Tau levels.
- PublicationFluorescence Sensors Based on Hydroxycarbazole for the Determination of Neurodegeneration-Related Halide Anions(MPDI, 2022-03-14) González Ruiz, Víctor; Cores, Ángel; Caja, M. Mar; Sridharan, Vellaisamy; Villacampa, Mercedes; Martín, M. Antonia; Olives Barba, Ana Isabel; Menéndez Ramos, José CarlosThe environmental presence of anions of natural origin or anthropogenic origin is gradually increasing. As a tool to tackle this problem, carbazole derivatives are an attractive gateway to the development of luminescent chemosensors. Considering the different mechanisms proposed for anion recognition, the fluorescence properties and anion-binding response of several newly synthesised carbazole derivatives were studied. Potential anion sensors were designed so that they combined the native fluorescence of carbazole with the presence of hydrogen bonding donor groups in critical positions for anion recognition. These compounds were synthesised by a feasible and non-expensive procedure using palladium-promoted cyclodehydrogenation of suitable diarylamine under microwave irradiation. In comparison to the other carbazole derivatives studied, 1-hydroxycarbazole proved to be useful as a fluorescent sensor for anions, as it was able to sensitively recognise fluoride and chloride anions by establishing hydrogen bond interactions through the hydrogen atoms on the pyrrolic nitrogen and the hydroxy group. Solvent effects and excited-state proton transfer (ESPT) of the carbazole derivatives are described to discard the role of the anions as Brönsted bases on the observed fluorescence behaviour of the sensors. The anion–sensor interaction was confirmed by 1H-NMR. Molecular modelling was employed to propose a mode of recognition of the sensor in terms of complex stability and interatomic distances. 1-hydroxycarbazole was employed for the quantitation of fluoride and chloride anions in commercially available medicinal spring water and mouthwash samples.
- PublicationEnhanced Stability and Bioactivity of Natural Anticancer Topoisomerase I Inhibitors through Cyclodextrin Complexation(MDPI, 2021-10-03) González Ruiz, Víctor; Cores Esperón, Ángel; Martín Cámara, Olmo; Orellana, Karen; Cervera Carrascón, Víctor Enrique; Michalska, Patrycja; León, Rafael; Olives Barba, Ana Isabel; Martín Carmona, M. Antonia; Menéndez Ramos, José CarlosThe use of cyclodextrins as drug nano-carrier systems for drug delivery is gaining importance in the pharmaceutical industry due to the interesting pharmacokinetic properties of the resulting inclusion complexes. In the present work, complexes of the anti-cancer alkaloids camptothecin and luotonin A have been prepared with β-cyclodextrin and hydroxypropyl-β-cyclodextrin. These cyclodextrin complexes were characterized by nuclear magnetic resonance spectroscopy (NMR). The variations in the 1H-NMR and 13C-NMR chemical shifts allowed to establish the inclusion modes of the compounds into the cyclodextrin cavities, which were supported by docking and molecular dynamics studies. The efficiency of the complexation was quantified by UV-Vis spectrophotometry and spectrofluorimetry, which showed that the protonation equilibria of camptothecin and luotonin A were drastically hampered upon formation of the inclusion complexes. The stabilization of camptothecin towards hydrolysis inside the cyclodextrin cavity was verified by the quantitation of the active lactone form by reverse phase liquid chromatography fluorimetric detection, both in basic conditions and in the presence of serum albumin. The antitumor activity of luotonin A and camptothecin complexes were studied in several cancer cell lines (breast, lung, hepatic carcinoma, ovarian carcinoma and human neuroblastoma) and an enhanced activity was found compared to the free alkaloids, particularly in the case of hydroxypropyl-β-cyclodextrin derivatives. This result shows that the cyclodextrin inclusion strategy has much potential towards reaching the goal of employing luotonin A or its analogues as stable analogues of camptothecin.
- PublicationIncidencia de las características geológicas y ambientales sobre la calidad de las aguas de los tramos superior y medio del río Arlanzón(Universidad Complutense de Madrid, Servicio de Publicaciones, 2002) Olives Barba, Ana Isabel; Castillo García, Benito del; Torija Isasa, María EsperanzaEl presente trabajo tiene como objetivo conocer el contenido de 7 metales esenciales: co, cr, cu, fe, mn, ni y zn, y 2 tóxicos: cd y pb. asi como sus variaciones estacionales, en las aguas del río arlanzón, desde su nacimiento en la sierra de la demanda hasta su llegada a la ciudad de Burgos. Estas aguas se destinan principalmente al abastecimiento público y regadío. Se realizaron 7 muestreos con frecuencia bimestral entre julio de 1991 y junio de 1992, tomando muestras en 21 puntos. Se determinaron estos metales por e.a.a., en sus contenidos tanto totales como disueltos. También se analizaron 6 parametros físico/químicos y 4 de contaminación que pueden influir en el contenido metalico. Los niveles medios anuales encontrados para los metales totales fueron gr (<1ppb)<100 ppb)
- PublicationEnsayo de inmersión del alumnado en el modelo IMRyD a través de la solicitud de un proyecto de investigación en un entorno de simulación(IATED - Proceedings of EDULEARN23 Conference - ISBN: 978-84-09-52151-7, 2023-07-19) Olives Barba, Ana Isabel; Caja López, María Del Mar; Coronel Gonzalo, Cristina; Gómez Gómez, Beatriz; López Ruiz, María Beatriz; Martín Carbajo, María Laura; Martín Carmona, María Antonia; Ortega Moreno, María; Rodrigo Martín, Rosa; Rodríguez Moreno, María del Pilar; Sanz Riomoros, Isabel; Urrestarazu Morocoima, José Antonio; Zapatero Martínez, MaríaSe ha desarrollado una actividad combinada de Aprendizaje Basado en Proyectos (ABP) y Aprendizaje Basado en Simulación (ABS) durante el primer semestre del curso 2022-23, dentro de la asignatura obligatoria de Química Analítica II, que se imparte en el segundo curso del Grado en Farmacia y del Doble Grado en Farmacia y Nutrición Humana y Dietética de la UCM. Según la metodología propuesta, los estudiantes partieron de la idea de desarrollar un nuevo método analítico para cuantificar un compuesto de su interés presente en muestras alimentarias, farmacéuticas, clínicas o ambientales. Para ello, elaboraron una propuesta y solicitaron un proyecto de investigación en una convocatoria simulada que lanzaron los profesores en el campus virtual de la asignatura. Al final del semestre, se celebró una sesión de pósteres en la que se invitó a los estudiantes a presentar su trabajo, de forma similar a un congreso. El objetivo principal de la actividad ha sido familiarizar a los estudiantes con el formato habitual de los documentos científicos (IMRD), concienciarles sobre cómo elaborar una propuesta con soporte bibliográfico y desarrollar habilidades de comunicación oral y escrita que necesitarán en su desarrollo profesional y, en un futuro próximo, en la elaboración de sus TFGs y TFMs. La actividad se diseñó como una tarea complementaria al programa de la asignatura. Participaron voluntariamente en la actividad 46 estudiantes de dos grupos de la asignatura (45%) y se presentaron 13 propuestas. La presentación de los carteles y la sesión informativa fueron especialmente participativas y brindaron a los estudiantes la oportunidad de desarrollar un pensamiento crítico al juzgar los pósteres. Los profesores valoraron la actividad como una herramienta prometedora para el desarrollo competencial de los estudiantes, que anima al profesorado como diseñadores de entornos de aprendizaje activo.