Person: Madrid Albarrán, María Yolanda
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First Name
María Yolanda
Last Name
Madrid Albarrán
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
Identifiers
38 results
Search Results
Now showing 1 - 10 of 38
Item Determination of phenolic compounds in residual brewing yeast using matrix solid-phase dispersion extraction assisted by titanium dioxide nanoparticles(Journal of Chromatography A, 2019) Gómez Mejía, Esther; Rosales Conrado, Noelia; León González, María Eugenia De; Madrid Albarrán, María Yolanda; ELSEVIERA simple and efficient low-cost matrix solid-phase dispersion (MSPD) extraction based on TiO2nanopar-ticles (NPs) and diatomaceous earth has been developed for the recovery of phenolic compounds fromresidual brewing yeast. Experimental conditions for MSPD extraction were optimized by an experimen-tal design approach. A screening factorial design plus replicates at the center point, followed by surfaceresponse analysis were used. The simultaneous identification and quantification of eleven main nat-ural polyphenols: caffeic, chlorogenic, p-coumaric, 3,4-dihydroxibenzoic, trans-ferulic and gallic acids,kaempferol, myricetin, naringin, quercetin and rutin, was possible by combining MSPD and capillaryliquid chromatography couple to a diode array detection system (cLC-DAD) and liquid chromatogra-phy couple to a triple quadrupole analyzer (LC–MS/MS). Moreover, residual brewing yeast extracts wereevaluated in terms of DPPH (1,1-diphenyl–2 picrylhydrazyl) free radical scavenging activity. Polyphenol-nanoparticle interaction was studied by UV–vis spectroscopy and electron transmission microscopy(TEM), pointing out a stable interplay that assists phenolic isolation. The extracted polyphenol quan-tities were within the 3.2-1,500 g g−1range, and the high antioxidant activity estimated suggested thatdeveloped MSPD is a successful, simple, efficient and rapid method for the extraction and recovery of bioactive phenolic compounds, which promotes the reuse and re-evaluation of brewing yeast agri-foodby-products.Item Aflatoxin detoxification by thermal cooking treatment and evaluation of in vitro bioaccessibility from white and brown rice(Food Chemistry, 2024) Romero Sánchez, Iván; Gracia Lor, Emma; Madrid Albarrán, María YolandaAflatoxins pose a severe risk to the human health. In this study, the detoxifying capacity of a thermal cooking treatment applied to white and brown rice spiked with aflatoxins B1, B2, G1 and G2 as well as the aflatoxin bioaccessibility in cooked rice after applying an in vitro digestion model was evaluated. The cooking treatment (boiling with water at 100 ◦C for 12 min) evidenced an important extraction capacity of the boiling water over aflatoxins (25 %-56 %), that was higher for brown rice. Moreover, aflatoxins G1 and G2 were unstable with losses around 35 %. The highest bioaccessibility percentage was obtained for white rice (60 %-83 %) compared to brown rice (28 %-47 %), due to aflatoxin losses from brown rice after the gastric step. These results confirm the potential of this thermal cooking treatment to reduce aflatoxins in rice and suggest the influence of the nutritional composition of each rice on aflatoxin behaviour.Item Selenium and tellurium-based nanoparticles as interfering factors in quorum sensing-regulated processes: violacein production and bacterial biofilm formation(Metallomics, 2019) Gómez Gómez, Beatriz; Arregui García-Roves, Lucía; Serrano Barrero, Susana Lourdes; Santos de la Sen, Antonio; Pérez Corona, María Teresa; Madrid Albarrán, María YolandaA cell-to-cell communication system called quorum sensing (QS) promotes the transcription of certain target genes in bacterial cells leading to the activation of different cellular processes, some of them related to bacterial biofilm formation. The formation of bacterial biofilms favours antibiotic resistance, which is nowadays a significant public-health problem. In this study, the effect of selenium (SeNPs) and tellurium (TeNPs) nanoparticles was examined in two bacterial processes mediated by QS: violacein production by Chromobacterium violaceum and biofilm formation by Pseudomonas aeruginosa. For this purpose, quantification of the pigment production in the presence of these nanoparticles was monitored using the C. violaceum strain. Additionally, a combination of different microscopical imaging techniques was applied to examine the changes in the 3D biofilm structure of P. aeruginosa, which were quantified through performing architectural metric calculations (substratum area, cell area coverage and biovolume). SeNPs produce an 80% inhibition in the violacein production by C. violaceum and a significant effect on the P. aeruginosa biofilm architecture (a reduction of 80% in the biovolume of the bacterial biofilm was obtained). TeNPs similarly affect violacein production and the P. aeruginosa biofilm structure but at lower concentration levels. The results obtained suggest an important disruption of the QS signalling system by SeNPs and TeNPs, supporting nanotechnology as a promising tool to fight against the emerging problem of bacterial resistance related to bacterial biofilm formation.Item Biotransformation of selenium by lactic acid bacteria: formation of seleno-nanoparticles and seleno-amino acids(Frontiers in Bioengineering and Biotechnology, 2020) Martínez, Fernando Gabriel; Moreno Martín, Gustavo; Pescuma, Micaela; Madrid Albarrán, María Yolanda; Mozzi, FernandaSelenium (Se) is an essential micronutrient for the majority of living organisms, and it has been identified as selenocysteine in the active site of several selenoproteins such as glutathione peroxidase, thioredoxin reductase, and deiodinases. Se deficiency in humans is associated with viral infections, thyroid dysfunction, different types of cancer, and aging. In several European countries as well as in Argentina, Se intake is below the recommended dietary Intake (RDI). Some lactic acid bacteria (LAB) can accumulate and bio-transform selenite (toxic) into Se-nanoparticles (SeNPs) and Se-amino acids (non-toxic). The microbial growth, Se metabolite distribution, and the glutathione reductase (involved in selenite reduction) activity of Se-enriched LAB were studied in this work. The ninety-six assayed strains, belonging to the genera Lactococcus, Weissella, Leuconostoc, Lactobacillus, Enterococcus, and Fructobacillus could grow in the presence of 5 ppm sodium selenite. From the total, eight strains could remove more than 80% of the added Se from the culture medium. These bacteria accumulated intracellularly between 1.2 and 2.5 ppm of the added Se, from which F. tropaeoli CRL 2034 contained the highest intracellular amount. These strains produced only the seleno-amino acid SeCys as observed by LC-ICP-MS and confirmed by LC-ESI-MS/MS. The intracellular SeCys concentrations were between 0.015 and 0.880 ppm; Lb. brevis CRL 2051 (0.873 ppm), Lb. plantarum CRL 2030 (0.867 ppm), and F. tropaeoli CRL 2034 (0.625 ppm) were the strains that showed the highest concentrations. Glutathione reductase activity values were higher when the strains were grown in the presence of Se except for the F. tropaeoli CRL 2034 strain, which showed an opposite behavior. The cellular morphology of the strains was not affected by the presence of Se in the culture medium; interestingly, all the strains were able to form spherical SeNPs as determined by transmission electron microscopy (TEM). Only two Enterococcus strains produced the volatile Se compounds dimethyl-diselenide identified by GC-MS. Our results show that Lb. brevis CRL 2051, Lb. plantarum CRL 2030, and F. tropaeoli CRL 2034 could be used for the development of nutraceuticals or as starter cultures for the bio-enrichment of fermented fruit beverages with SeCys and SeNPs.Item Fate and effect of in-house synthesized tellurium based nanoparticles on bacterial biofilm biomass and architecture. Challenges for nanoparticles characterization in living systems(Science of the Total Environment, 2020) Gómez Gómez, Beatriz; Sanz Landaluce, Jon; Pérez Corona, María Teresa; Madrid Albarrán, María YolandaThe unexpected impact of nanoparticles on environment and human health remains as a matter of concern. In this sense, understanding the interaction between nanoparticles and biological indicators such as microorganism may help to understand their fate and effect in environmental systems. However, the adverse effect of nanoparticles greatly depends on their properties and, therefore, a precise evaluation of nanoparticles physicochemical characteristics is mandatory as the first step in accurately elucidating their behaviour in different ecosystems. Here in this work, in house-synthesized tellurium-based nanoparticles have been fully characterized for first time by means of a multi-method approach. Once characterized, the effect of these nanoparticles on Staphylococcus aureus and Escherichia coli biofilm biomass and structure was explored and quantified for first time. Moreover, the morphological transformations of tellurium based nanoparticles within the confines of a biofilm are also highlighted. Architectural metric calculations evidenced that nanoparticles were able to reduce the biovolume of the biofilm produced for both bacteria. Interestingly, the interaction between nanoparticles and bacterial communities led to the transformation of telluriumnanoparticles from sphere to rod-shaped nanoparticles. These findings open new insights into the behaviour of a type of uncommon nanoparticles such as tellurium-based nanoparticles on microbial communitiesItem Exploring the Behavior and Metabolic Transformations of SeNPs in Exposed Lactic Acid Bacteria. Effect of Nanoparticles Coating Agent(International Journal of Molecular Sciences, 2017) Palomo Siguero, María; Madrid Albarrán, María YolandaThe behavior and transformation of selenium nanoparticles (SeNPs) in living systems such as microorganisms is largely unknown. To address this knowledge gap, we examined the effect of three types of SeNP suspensions toward Lactobacillus delbrueckii subsp. bulgaricus LB-12 using a variety of techniques. SeNPs were synthesized using three types of coating agents (chitosan (CS-SeNPs), hydroxyethyl cellulose (HEC-SeNPs) and a non-ionic surfactant, surfynol (ethoxylated-SeNPs)). Morphologies of SeNPs were all spherical. Transmission electron microscopy (TEM) was used to locate SeNPs in the bacteria. High performance liquid chromatography (HPLC) on line coupled to inductively coupled plasma mass spectrometry (ICP-MS) was applied to evaluate SeNP transformation by bacteria. Finally, flow cytometry employing the live/dead test and optical density measurements at 600 nm (OD600) were used for evaluating the percentages of bacteria viability when supplementing with SeNPs. Negligible damage was detected by flow cytometry when bacteria were exposed to HEC-SeNPs or CS-SeNPs at a level of 10 μg Se mL−1. In contrast, ethoxylated-SeNPs were found to be the most harmful nanoparticles toward bacteria. CS-SeNPs passed through the membrane without causing damage. Once inside, SeNPs were metabolically transformed to organic selenium compounds. Results evidenced the importance of capping agents when establishing the true behavior of NPs.Item Insights into the accumulation and transformation of Ch-SeNPs by Raphanus sativus and Brassica juncea: effect on essential elements uptake(Science of the Total Environment, 2020) Moreno Martín, Gustavo; Sanz Landaluce, Jon; León González, María Eugenia De; Madrid Albarrán, María Yolanda; ElsevierSelenium (Se) at very low doses has important functions for humans. Unfortunately, the low levels of Se in soils in various regions of the world have implemented the agronomic biofortification of crops by applying Se-enriched fertilizers (mainly based on selenate). Lately, the use of nanofertilizers is growing in interest as their low size reduces the amount of chemicals and minimizes nutrient losses in comparison with conventional bulk fertilizers. However, the knowledge on their fate and environmental impact is still scarce. This study aims to evaluate the biotransformation of chitosan-modified Se nanoparticles (Ch-SeNPs) as well as their effect on the metabolism of essential metals (Fe, Cu, Zn and Mo) when applied to hydroponic cultivation of R. sativus and B. juncea. In house-synthesized Ch-SeNPs were characterized in both synthesis and hydroponic culture media by transmission electron microscopy (TEM), dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). The composition of one-tenth strength Hoagland's solution did not affect the size, shape and concentration in number of particles per mL of Ch-SeNPs. The plants were grown inside a box at 25 °C during the months of May–July in 2018. After a week of treatment with Ch-SeNPs, plants were harvested and divided into roots and aerial part. The biotransformation of Ch-SeNPs was evaluated through a process of enzymatic hydrolysis and subsequent analysis by HPLC-ICP-MS and HPLC-ESI-MS/MS. The results confirmed the transformation of Ch-SeNPs to seleno-amino acids: Selenomethionine (SeMet), Semethylselenocysteine (SeMetSeCys) and ɣ-glutamyl-Se-MetSeCys. Moreover, Multiple-way analysis of variance (ANOVA) and principal component analysis (PCA) showed that, regardless the plant species, Ch-SeNPs supplementation affected the absorption of Zn.Item Characterization of AgNPs and AuNPs in sewage sludge by single particle inductively coupled plasma-mass spectrometry(Talanta, 2022) Moreno Martín, Gustavo; Gómez Gómez, Beatriz; León González, María Eugenia De; Madrid Albarrán, María Yolanda; ElsevierThis study develops for the first time an analytical method for the characterization of silver and gold nanoparticles in sewage sludge. The evaluation of the effect of temperature, extracting agent and centrifugation speed and time on the extraction yield was carried out through a multifactorial analysis of variance which allows us to select 289 g, 5 min and 20 mM sodium pyrophosphate tetrabasic as optimal extraction conditions. Under these conditions, the analysis of the extract by single particle inductively coupled plasma-mass spectrometry provided recovery percentages of 70 ± 2% and 56 ± 1% for silver and gold nanoparticles, respectively. Moreover, the complementary results obtained upon analysis of these extracts by transmission electron microscopy and single particle inductively coupled plasma-mass spectrometry showed that the developed method did not modify the original size and shape of these nanoparticles during the extraction procedure. Size detection limits of 23 nm and 16 nm as well as number concentration limits of 3.12 × 109 particles kg−1 and 1.38 × 109 particles kg−1 were obtained for silver and gold nanoparticles, respectively. Moreover, a stability study of silver and gold nanoparticles in sewage sludge for 12 months showed differences between the two nanoparticle types. Although the sizes were not affected during the 12 months, silver nanoparticles underwent an oxidation process from 6 months onwards, which was reflected in an increase in the percentage of ionic silver from 14 ± 1% at 6 months to 24 ± 2% at 12 months. The developed methodology represents a simple, reliable and fast tool for detecting, quantifying and assessing the stability of nanoparticles in an important environmental sample such as sewage sludge.Item Valorization of citrus reticulata blanco peels to produce enriched wheat bread: phenolic bioaccessibility and antioxidant potential(ANTIOXIDANTS, 2023) Gómez Mejía, Esther; Sacristán, Iván; Rosales Conrado, Noelia; León González, María Eugenia De; Madrid Albarrán, María YolandaThe fortification of foods with bioactive polyphenols aims to improve their functional properties and to provide health benefits. Yet, to exert their benefits, phenolic compounds must be released from the food matrix and absorbed by the small intestine after digestion, so assessing their bioaccessibility is crucial to determine their potential role. This work aims to incorporate Citrus reticulata Blanco peel extracts into wheat bread as a promising opportunity to increase their bioactive potential, along with supporting the sustainable management of citrus-industry waste. A control and a wheat bread enriched at 2% and 4% (w/v) with a phenolic extract from mandarin peels were prepared and analyzed for antioxidant activity and phenolic composition using LC-MS and UV-Vis spectrophotometry. In addition, in vitro digestion was performed, and the digested extracts were analyzed with HPLC-MS/MS. The results showed a significant increase in total flavonoid content (TFC, 2.2 ± 0.1 mg·g−1), antioxidant activity (IC50 = 37 ± 4 mg·g−1), and contents of quercetin, caffeic acid, and hesperidin in the 4% (w/v) enriched bread. Yet, most polyphenols were completely degraded after the in vitro digestion process, barring hesperidin (159 ± 36 μg·g−1), highlighting the contribution of citrus enrichment in the development of an enriched bread with antioxidant potential.Item Simultaneous determination of the size and concentration of AgNPs in water samples by UV–vis spectrophotometry and chemometrics tools(Talanta, 2018) Moreno Martín, Gustavo; León González, María Eugenia De; Madrid Albarrán, María YolandaThe combination of UV–vis spectrophotometry with a chemometric calibration tool based on partial least squares (PLS) has allowed us the development of a multivariate analytical method that simultaneously estimates the concentration and size of mixtures of silver nanoparticles (AgNPs) in environmental water samples. The method is based on changes in the surface plasmon resonance band (SPRB) of AgNPs when they form aggregated/assembled structures with L-cysteine (L-cys). Measurementts were performed by employed a fixed-time kinetics method that implies that the final spectra (response) are obtained by subtstracting the solutions spectra at fixed times. Optimization of experimental conditions affecting aggregation such as time, temperature, pH and concentration of aggregating substance was performed by experimental design and response surface methodologies (RSM). A multivariate calibration model using AgNPs of known diameter size ((20 ± 3), (41 ± 3), (59 ± 5) and (79 ± 7) nm) within a concentration range between 0.62 and 2.5 mg L−1 was constructed by using a mixture experimental design and PLS. The method was finally applied to estimate size and concentration of AgNPs in AgNPs-spiked river and tap water samples. Water samples were spiked with individual, binary and ternary mixtures of AgNPs of different sizes and by using two types of AgNPs: citrate-coated AgNPs (cit-AgNPs) and polyvinylpyrrolidone-coated AgNPs (PVP-AgNPs). A good correspondence was obtained between predicted values and the total amount of AgNPs added with recovery values ranged within 80–160% for the individual mixtures, 68–108% for the binary mixtures and 60–64% for the ternary mixtures of AgNPs. Finally, transmission electron microscopy (TEM) measurements were performed for those cases where discrepancies between the expected and the obtained values were observed. TEM micrographs evidenced the presence of agglomerates or aggregates of AgNPs in some of the mixtures or water tested.