Person:
San Segundo Acosta, Pablo

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First Name
Pablo
Last Name
San Segundo Acosta
Affiliation
Universidad Complutense de Madrid
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Now showing 1 - 9 of 9
  • Publication
    Identification of tumor-associated antigens with diagnostic ability of colorectal cancer by in-depth immunomic and seroproteomic analysis
    (Elsevier, 2020-03-01) Garranzo Asensio, María; San Segundo Acosta, Pablo; Povés Francés, Carmen; Fernández Aceñero, María Jesús; Martínez Useros, Javier; Montero Calle, Ana; Solís Fernández, Guillermo; Sánchez Martínez, Mari Cruz; Rodríguez, Nuria; Cerón, María Ángeles; Fernández Díez, Servando; Domínguez Muñóz, Gemma; Ríos, Vivian de los; Peláez García, Alberto, Alberto; Guzmán Aránguez, Ana Isabel; Barderas, Rodrigo
    Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer related death worldwide. Its diagnosis at early stages would significantly improve the survival of CRC patients. The humoral immune response has been demonstrated useful for cancer diagnosis, predating clinical symptoms up to 3 years. Here, we employed an in-depth seroproteomic approach to identify proteins that elicit a humoral immune response in CRC patients. The seroproteomic approach relied on the immunoprecipitation with patient-derived autoantibodies of proteins from CRC cell lines with different metastatic properties followed by LC-MS/MS. After bioinformatics, we focused on 31 targets of CRC autoantibodies. After WB and IHC validation, ERP44 and TALDO1 showed potential to discriminate disease-free and metastatic CRC patients, and time to recurrence of CRC patients in stage II. Using plasma samples of 30 healthy individuals, 28 premalignant individuals, and 32 CRC patients, nine out of 13 selected targets for seroreactive analysis showed significant diagnostic ability to discriminate either CRC patients or premalignant subjects from controls. Our results suggest that the here defined panel of CRC autoantibodies and their target proteins should be included in CRC blood-based biomarker panels to get a clinically useful blood-based diagnostic signature for CRC detection. Significance: Colorectal cancer is one of the deadliest cancer types mainly due to its late diagnosis. Its early diagnosis, therefore, is of great importance since it would significantly improve the survival of CRC patients. In our work, the in-depth seroproteomic analysis of colorectal cancer using isolated IgGs from colorectal cancer patients and controls and protein extract of colorectal cancer cells provide the identification of valuable biomarkers with diagnostic and prognostic ability of the disease.
  • Publication
    Química Inspirada por la Naturaleza: Lecciones en el Museo Nacional de Ciencias Naturales
    (2017) Batanero Cremades, Eva; Yélamos López, Belén; Fernández Fernández, Mª Inmaculada; López García-Gallo, Pilar; López Rodríguez, Juan Carlos; San Segundo Acosta, Pablo
    Uno de los principales problemas con los que se enfrentan los profesores de Biología es el escaso interés que despierta esta asignatura en los estudiantes universitarios de algunas disciplinas -como química, física, arquitectura o ingeniería- al considerarla una asignatura difícil de aprender y de poca utilidad. Así, motivación, comprensión e interdisciplinaridad son tres retos a los que se enfrenta el profesorado de la asignatura de Biología del Grado en Química. El objetivo general del proyecto ha sido contribuir a la mejora de la enseñanza/aprendizaje de la asignatura de Biología. Para ello el profesorado ha implementado en el aula, y de forma combinada, dos metodologías: La "Biomímesis" y "La enseñanza/aprendizaje basada en Proyectos", que han demostrado ser herramientas muy útiles en el aula, para despertar la motivación y el interés de los estudiantes por el aprendizaje de Biología. Por un lado, al implicarles en su propio proceso de aprendizaje, los estudiantes han tenido un papel protagonista al ser los responsables del desarrollo de proyectos interesantes y retadores, elegidos por ellos mismos. Por otro lado, les ha permitido ver la aplicación o transferencia sostenible de lo aprendido en el aula a la sociedad y el medio ambiente. A estas dos metodologías didácticas se ha unido una tercera: Los Museos de Ciencia Naturales como espacios de enseñanza/aprendizaje de ciencia por investigación. Un total de 2 grupos de teoría han participado en esta experiencia docente. Los trabajos realizados han sido expuestos a otros estudiantes (en el aula) y al público general (en el Museo Nacional de Ciencias Naturales).
  • Publication
    Proteomics techniques for the identification of new biomarkers in chronic disease: alzheimer's disease and allergy to olive pollens as models
    (Universidad Complutense de Madrid, 2021-02-05) San Segundo Acosta, Pablo; Barderas Manchado, Rodrigo; Villalba Díaz, María Teresa
    The term “biomarker” can be defined as any characteristic which can be objectively measured and evaluated as an indicator of normal biological processes, pathogenic processes, orpharmacological responses to a therapeutic intervention. Biomarkers can be highly diverse, and include specific molecules such as proteins (protein biomarkers), which can be used in screening tests for diagnosis. Proteomics consists of the large-scale characterization of all the expressed proteins in a cell, tissue or living organism at a specific moment and condition, using techniques such as two-dimensional electrophoresis (2-DE), mass-spectrometry (MS) and protein microarrays. During the last years, these high-throughput screening techniques have been extensively used for the discovery of multiple protein biomarkers, as they allow for the identification and quantification of a high number of proteins in a single sample and with high sensitivity. Alzheimer’s disease (AD) is a chronic, progressive and unremitting neurodegenerative disorder affecting fundamental brain areas for memory and cognition, mainly the neocortex and the hippocampus. It is the most common form of dementia worldwide, with an estimated prevalence between 10% and 30% in the aging population which is expected to double in the next years. Two main histopathological hallmarks define AD: extracellular amyloid β peptide (Aβ) deposits known as amyloid plaques, and intracellular aggregates of hyperphosphorylated tau known as neurofibrillary tangles (NFTs). Currently, the only useful biomarkers for AD diagnosis are based on cerebrospinal fluid (CSF) analysis and neuroimaging techniques, which restricts their use to specialty clinics. Therefore, AD diagnosis is mainly based on clinical symptoms, sometimes leading to misdiagnosis. Besides, this method fails to detect the disease at early stages. To counter these problems, new biomarkers useable in primary care settings, such as blood-based biomarkers, are needed in the field and are the focus of many research groups worldwide. Allergic diseases, also known as type I hypersensitivity disorders, constitute another group of chronic diseases whose prevalence has alarmingly increased in the population of developed countries. It is estimated that up to 25% of the population of these countries suffer from respiratory allergies such as rhinitis, conjunctivitis and asthma, which involve the production of high-affinity Immunoglobulin E (IgE) antibodies against generally harmless environmental antigens known as aeroallergens. While in tropical areas mites are the main cause of respiratory allergies, in North America and Europe pollen plays a major role in allergic sensitization (pollinosis). In Spain, olive (Olea europaea L.) pollen is the second leading cause of pollinosis after grass pollen, becoming the first one in regions like Andalusia, where olive is extensively cultivated. Besides, the continuous advertising of the Mediterranean diet has boosted olive cultivation in regions of the United States, Japan, China, South Africa and South America, where it will presumably become a main allergenic source. Although olive pollen allergens have been deeply characterized during the last years, some allergens potentially relevant for the diagnosis and management of this allergy have remained unidentified due to technical restrictions and limited genomics and proteomics data...
  • Publication
    “¿Qué es lo que sabemos… sobre Biología”
    (2020-05-18) Batanero Cremades, Eva; Rodríguez Crespo, José Ignacio; San Segundo Acosta, Pablo; Bueno Díaz, Cristina; Parrón Ballesteros, Jorge
    Dada la importancia del inglés y de las tecnologías móviles hoy día, los estudiantes utilizarán dichas tecnologías para reforzar el aprendizaje de Biología, y motivarlos a estudiar, mediante dos actividades: crear test Kahoot en inglés y concursar.
  • Publication
    Phage-Derived and Aberrant HaloTag Peptides Immobilized on Magnetic Microbeads for Amperometric Biosensing of Serum Autoantibodies and Alzheimer's Disease Diagnosis
    (Wiley-VCH, 2021-07-27) Valverde de la Fuente, Alejandro; Montero Calle, Ana; Arévalo Pérez, Beatriz; San Segundo Acosta, Pablo; Serafín González-Carrato, Verónica; Alonso Navarro, Miren; Solís Fernández, Guillermo; Pingarrón Carrazón, José Manuel; Campuzano Ruiz, Susana; Barderas Manchado, Rodrigo
    An electrochemical biosensing platform for serum autoantibodies (AAbs) detection is reported in this work, exploiting for the first time six Alzheimer's disease (AD)-specific phage-derived and frameshift aberrant HaloTag peptides as receptors, immobilized on magnetic microbeads (MBs) surface and captured on disposable electrodes to perform amperometric detection. Operational analytical characteristics and clinical diagnostic ability of the bioplatform were probed in optimized key experimental conditions by analysing serum AAbs of AD patients and healthy subjects. The value of 100% obtained for AUC, sensitivity, and selectivity from the all peptides combined ROC curve, indicate full AD-diagnostic capability of the methodology, which was further implemented, as proof of concept, in a POC multiplexing platform to detect the signature in a single test over clinically actionable times (1 h 15 min), opening great promise for the type of diagnosis and AD patients’ monitoring follow-up currently pursued.
  • Publication
    Química Inspirada por la Naturaleza: Lecciones en el Museo Nacional de Ciencias Naturales II
    (2018-06-04) Batanero Cremades, Eva; Yélamos López, Belén; Fernández Fernández, María Inmaculada; López García-Gallo, Pilar; López-Rodríguez, Juan Carlos; San Segundo Acosta, Pablo; Rivera de Torre, Esperanza; Liñan Vargas, Katia Estefanía
    El proyecto Innova-Docencia 70 es una continuación del proyecto Innova-Docencia 30 que se desarrolló durante el curso académico 2016-2017 y su objetivo principal es implementar una nueva estrategia de enseñanza/aprendizaje que motive a los estudiantes hacia el estudio de Biología, favorezca la comprensión de los contenidos y les permita conectar los nuevos conocimientos adquiridos con el mundo real que les rodea. Motivación, comprensión e interdisciplinaridad son tres grandes retos a los que se enfrenta el profesorado de la asignatura de Biología del Grado en Química, que se imparte durante el primer cuatrimestre y que es de carácter obligatorio para los estudiantes de primero (6 ECTS), dado el escaso interés que despierta esta asignatura en los estudiantes de esta disciplina (además de otras, como física, arquitectura etc.), al considerarla una asignatura difícil de aprender y de poca utilidad. Esta estrategia docente tiene como eje metodológico principal la Biomímesis, pero a su vez se apoya en otras dos metodologías: (1) La enseñanza/aprendizaje basada en Proyectos y (2) Los Museos como espacios de enseñanza/aprendizaje. Todas ellas comparten un mismo principio: motivar al estudiante a aprender, implicándole en su propio proceso de aprendizaje. El estudiante ha de planificar, desarrollar y evaluar un proyecto (de carácter interdisciplinar) que le interesa, al ser elegido por él, y que le permite conectar lo académico con la vida.
  • Publication
    Identification of tumor-associated antigens with diagnostic ability of colorectal cancer by in-depth immunomic and seroproteomic analysis
    (Elsevier, 2020-03) Garranzo Asensio, María; San Segundo Acosta, Pablo; Povés Francés, Carmen; Fernández Aceñero, María Jesús; Martínez Useros, Javier; Montero Calle, Ana; Solís Fernández, Guillermo; Sánchez Martínez, Maricruz; Rodríguez Salas, Nuria; Cerón, María Ángeles; Fernández Díez, Servando; Domínguez Muñóz, Gemma; Ríos, Vivian de los; Peláez García, Alberto, Alberto; Guzmán Aránguez, Ana Isabel; Barderas Manchado, Rodrigo
    Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer related death worldwide. Its diagnosis at early stages would significantly improve the survival of CRC patients. The humoral immune response has been demonstrated useful for cancer diagnosis, predating clinical symptoms up to 3 years. Here, we employed an in-depth seroproteomic approach to identify proteins that elicit a humoral immune response in CRC patients. The seroproteomic approach relied on the immunoprecipitation with patient-derived autoantibodies of proteins from CRC cell lines with different metastatic properties followed by LC-MS/MS. After bioinformatics, we focused on 31 targets of CRC autoantibodies. After WB and IHC validation, ERP44 and TALDO1 showed potential to discriminate disease-free and metastatic CRC patients, and time to recurrence of CRC patients in stage II. Using plasma samples of 30 healthy individuals, 28 premalignant individuals, and 32 CRC patients, nine out of 13 selected targets for seroreactive analysis showed significant diagnostic ability to discriminate either CRC patients or premalignant subjects from controls. Our results suggest that the here defined panel of CRC autoantibodies and their target proteins should be included in CRC blood-based biomarker panels to get a clinically useful blood-based diagnostic signature for CRC detection. Significance: Colorectal cancer is one of the deadliest cancer types mainly due to its late diagnosis. Its early diagnosis, therefore, is of great importance since it would significantly improve the survival of CRC patients. In our work, the in-depth seroproteomic analysis of colorectal cancer using isolated IgGs from colorectal cancer patients and controls and protein extract of colorectal cancer cells provide the identification of valuable biomarkers with diagnostic and prognostic ability of the disease.
  • Publication
    Biophysical and biological impact on the structure and IgE-binding of the interaction of the olive pollen allergen Ole e 7 with lipids
    (Elsevier, 2020-03-04) Carmen Oeo-Santos; López Rodríguez, Juan Carlos; García Mouton, Cristina; San Segundo Acosta, Pablo; Aurora Jurado; Carmen Moreno-Aguilar; García Álvarez, María Begoña; Pérez Gil, Jesús; Villalba Díaz, María Teresa; Barderas Manchado, Rodrigo; Cruz Rodríguez, Antonio
    Ole e 7 allergen from Olea europaea pollen possesses a major clinical relevance because it produces severe symptoms, such as anaphylaxis, in allergic patients exposed to high olive pollen counts. Ole e 7 is a non-specific lipid transfer protein (nsLTP) characterized by the presence of a tunnel-like hydrophobic cavity, which may be suitable for hosting and, thus, transporting lipids -as it has been described for other nsLTPs-. The identification of the primary amino acid sequence of Ole e 7, and its production as a recombinant allergen, allowed characterizing its lipid-binding properties and its effect at air-liquid interfaces. Fluorescence and interferometry experiments were performed using different phospholipid molecular species and free fatty acids to analyse the lipid-binding ability and specificity of the allergen. Molecular modelling of the allergen was used to determine the potential regions involved in lipid interaction. Changes in Ole e 7 structure after lipid interaction were analysed by circular dichroism. Changes in the IgE binding upon ligand interaction were determined by ELISA. Wilhelmy balance measurements and fluorescence surfactant adsorption tests were performed to analyse the surface activity of the allergen. Using these different approaches, we have demonstrated the ability of Ole e 7 to interact and bind to a wide range of lipids, especially negatively charged phospholipids and oleic acid. We have also identified the protein structural regions and the residues potentially involved in that interaction, suggesting how lipid-protein interactions could define the behaviour of the allergen once inhaled at the airways.
  • Publication
    Fast Electrochemical miRNAs Determination in Cancer Cells and Tumor Tissues with Antibody-Functionalized Magnetic Microcarriers
    (American Chemical Society, 2016-06-10) Torrente Rodríguez, Rebeca Magnolia; Ruiz Valdepeñas Montiel, Víctor; Campuzano Ruiz, Susana; Farchado-Dinia, Meryem; Barderas Manchado, Rodrigo; San Segundo Acosta, Pablo; Montoya, Juan J.; Pingarrón Carrazón, José Manuel
    Microribonucleic acids (miRNAs) have been linked with various regulatory functions and diseases and constitute important targets in future medical diagnostics and prognostics. We report here a novel sensitive and rapid bioelectrochemical strategy for miRNA determination. This strategy involves the development of a sensing approach making use of magnetic beads (MBs) modified with a specific DNA-RNA antibody as capture bioreceptor and amperometric detection implying the H2O2/hydroquinone (HQ) system at disposable screen-printed carbon electrodes (SPCEs). The developed biosensor exhibits a dynamic range from 8.2 to 250 pM and a detection limit of 2.4 pM (60 amol) of a synthetic target without any amplification step in 2 h. The usefulness of the approach was evaluated by analyzing total RNA (RNAt) extracted from metastatic cancer cell lines and human tumor tissues, which demonstrated its potential to perform determination of mature miRNAs in these complex samples. Moreover, the feasibility of the developed methodology to detect simultaneously the expression of two different miRNAs at dual SPCEs (SPdCEs) in one single experiment was also explored. The feasibility to capture and release target miRNAs make the developed methodology also an attractive tool to isolate, purify, and determine target miRNAs with great applicability in the clinical field.