Person: Benítez Del Castillo Sánchez, José Manuel
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First Name
José Manuel
Last Name
Benítez Del Castillo Sánchez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Inmunología, Oftalmología y ORL
Area
Oftalmología
Identifiers
16 results
Search Results
Now showing 1 - 10 of 16
Item Nuevo instrumento para medir la sensibilidad al contraste sin y con deslumbramiento(Archivos de la Sociedad Española de Oftalmología, 2003) Sánchez Ramos, Celia; Puell Marín, María Cinta; Pérez Carrasco, María Jesús; Langa Moraga, Antonio; Benítez Del Castillo Sánchez, José ManuelOBJETIVO/MÉTODO: valorar la sensibilidad al contraste fotópica, sin y con deslumbramiento con un nuevo instrumento. Se estudiaron 78 ojos derechos de 49 adultos jóvenes y de 29 adultos entre 35 y 55 años. La sensibilidad al contraste se midió con el Contrast Glarester CGT-1000 mediante una estrategia automatizada de reconocimiento para seis frecuencias espaciales. RESULTADOS/CONCLUSIONES: Los valores de sensibilidad al contraste fueron semejantes a los obtenidos con otros test clínicos. El aumento de la edad disminuyó significativamente la sensibilidad al contraste sin y con deslumbramiento. Sin embargo, el deslumbramiento no modificó la sensibilidad al contraste fotópica.Item Contrast sensitivity and disability glare in patients with dry eye(Acta Ophthalmologica Scandinavica, 2006) Puell Marín, María Cinta; Benítez Del Castillo Sánchez, José Manuel; Martínez De La Casa Fernández-Borrella, José María; Sánchez Ramos, Celia; Aladro Vico, Eva; Pérez Carrasco, María Jesús; Pedraza Aranda, Constanza; Hierro Zarzuelo, Almudena, delPurpose: To evaluate contrast sensitivity and disability glare in patients with dry eye using the Contrast Glaretester 1000. Methods: Contrast sensitivity and disability glare were determined in 33 eyes of 33 patients with dry eye and 30 eyes of 30 healthy control subjects for six target sizes with a visual angle of 6.3-0.7 degrees using the Contrast Glaretester 1000, whose working mechanism is similar to that of the conventional perimetry instrument. Results: Contrast sensitivity was significantly worse in dry eye group when viewing all target sizes (reduction of 0.10-0.25 log contrast units, p < 0.01) except at 6.3 degrees. In the presence of glare, differences in log contrast sensitivity between the groups (0.10-0.25 units) were significant (p < 0.01) for all target sizes, with the dry eye group showing worse results. The reduction in contrast sensitivity induced by glare (disability glare) was significantly worse in the dry eye group versus the control group but only for the 2.5-degree size target, where 0.14 log contrast units were lost. Conclusions: Contrast sensitivity with and without glare was significantly reduced in patients with dry eye compared with control subjects, but the number of log contrast units lost with glare (disability glare) was similar in the two groups, except for the 2.5-degree size target.Item Tear cytokine profile of glaucoma patients treated with preservative-free or preserved latanoprost(Ocular surface, 2017) Martínez De La Casa Fernández-Borrella, José María; Pérez Bartolomé, Francisco; Urcelay, Elena; Santiago, José Luis; Moreno Montañes, Javier; Arriola Villalobos, Pedro; Benítez Del Castillo Sánchez, José Manuel; García Feijoo, JuliánPurpose: To determine variations in cytokine levels of glaucoma patients treated either with preservative-free latanoprost or preserved latanoprost, relative to healthy individuals. Methods: Tear samples were collected from 39 healthy subjects, 20 glaucoma patients treated with preserved latanoprost, and 20 patients treated with preservative-free latanoprost. A set of 27 inflammatory cytokines was analyzed in each group, including interleukin (IL)-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, eotaxin, fibroblast growth factor (FGF) basic, granulocyte colony stimulating factor (G-CSF), granulocyte monocyte colony stimulating factor (GM-CSF), interferon (IFN)-γ, interferon gamma-induced protein (IP)-10, monocyte chemo attractant protein (MCP)-1MCAF, macrophage inflammatory protein (MIP)-1α, MIP-1β, platelet-derived growth factor (PDGF)-BB, regulated on activation, normal T cell expressed and secreted (RANTES), tumor necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF). Cytokine concentrations were obtained by the Bio-Plex Human Cytokine Immunoassay. Non-invasive tear breakup time (NI-TBUT), tear meniscus height, corneal fluorescein staining, conjunctival hyperemia and ocular surface disease index (OSDI) were assessed in patients treated with preservative-free and preserved latanoprost. Results: The levels of IL-2, IL-5, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, FGF basic, PDGF-BB, and TNF-α were significantly higher in patients receiving preserved latanoprost, compared to normal controls (p < 0.05). The expression of all the cytokines studied remained statistically invariable in patients receiving preservative-free latanoprost, compared to healthy subjects (p > 0.05). Ocular surface parameters were not significantly different in both glaucoma groups, and no correlation between these clinical parameters and cytokine levels was observed. Conclusions: Treatment with preserved latanoprost has a direct impact on tear cytokine levels, whereas this effect is not observed upon preservative-free latanoprost instillation.Item Comparison of the in vitro tolerance and in vivo efficacy of traditional timolol maleate eye drops versus new formulations with bioadhesive polymers(2011) Andrés Guerrero, Vanesa; Vicario De La Torre, Marta; Molina Martínez, Irene Teresa; Benítez Del Castillo Sánchez, José Manuel; García Feijoo, Julián; Herrero Vanrell, María Del RocíoPURPOSE. To assess the in vitro tolerance and in vivo efficacy of new unpreserved formulations of timolol maleate (TM) in aqueous solutions of bioadhesive polymers used for dry eye treatment and to compare them with three traditional TM formulations: unpreserved Timabak (Thea, Madrid, Spain), benzalkonium chloride (BAK)-preserved Timoftol (Frosst Laboratories, Madrid, Spain), and BAK-preserved Timolol Sandoz (Frosst Laboratories). METHODS. New formulations were composed of TM (0.5%) and carboxymethyl cellulose (0.5%), hyaluronic acid (0.2%), or hydroxypropylmethyl cellulose (0.3% or 0.5%). In vitro tolerance was determined in human corneal-limbal epithelial cells and normal human conjunctival cells. The ocular hypotensive effect was evaluated measuring IOP in rabbit eyes for 8 hours. RESULTS. In all cases, cell survival after exposure to the formulations was greater in the new unpreserved TM formulations than in the traditional TM solutions (BAK-preserved and unpreserved). In addition, the new formulations were demonstrated to maintain the hypotensive effect of TM in different magnitudes. The maximum hypotensive effect was reached by TM 0.5% in carboxymethyl cellulose 0.5% (32.37%). CONCLUSIONS. The results demonstrated that new unpreserved formulations of TM with bioadhesive polymers decreased IOP in rabbits and reached values closer to those reached by traditional solutions. Furthermore, new formulations presented a significantly higher in vitro tolerance than the same compound in traditional formulations. Although unpreserved formulations are usually more expensive, preservative-free antiglaucoma eye drops should improve compliance and adherence in the medical treatment of glaucoma. Bioadhesive polymers could be part of antiglaucoma formulations to reduce ocular toxicity, improve drug efficacy, and protect the ocular surface in long-term therapies.Item Mesopic contrast sensitivity and glare disability in drivers.(2003) Sánchez Ramos, Celia; Puell Marín, María Cinta; Pérez Carrasco, María Jesús; Langa Moraga, Antonio; Benítez Del Castillo Sánchez, José ManuelVisual Acuity is a poor predictor of driving ability and it is likely to be a poor predictor of many aspects of real-world vision, because the real world is not composed purely of fine objects with sharp edges at high contrast. Although not all objects are at contrst treshold either, it has been shown that a loss of contrast sensitivity (CS) is roughly equivalent to a loss of image contrast in the normal eye (OWSLEY, 1994). The extent of contrast loss required before real-world performance is reduced depends on the real-world task being performed. Somo tasks, such as optimal speed reading and mobility orientation in a well-llit room, tolerate large reductions in contrast (Elliot, 1996; Leffe, 1993; Pelli, 1986). These tasks would probably only be affected in patients with a severe loss of contrast sensitivity. However, other tasks such as speed reading newspaper-size print and face recognition are moderately affected by contrast reduction (Elliot, 1996). Further, mobility orientation under dim illumination has been shown to be seriously affected by reduced contrast. Thus, under low-iluminance conditions and when a subject is working near his or her acuity limit, tolerance to contrast loss is reduced (Legge, 1993)Item Efecto del hialuronato sódico (0.15%) en la sensibilidad al contraste en ojo seco con déficit hormonal.(2005) Sánchez Ramos, Celia; Llorens Casado, María Belén; Moral Martínez, I.; Puell Marín, María Cinta; Gonzalvo, M.T.; Pérez Carrasco, María Jesús; Langa Moraga, Antonio; Benítez Del Castillo Sánchez, José Manuel; Sáenz Francés, F.Los cambios de la actividad metabólica inducidos por las variaciones hormonales producen síntomas asociados, como la patología del "ojo seco". El tratamiento más frecuente para la sequedad ocular es la aplicación de lágrimas artificiales. El hialuronato sódico es uno de los fármacos que reconstruye la película lagrimal. No obstante, se desconoce su efecto en la percepción visual, especialmente en los que respeta a su posible repercusión en la sensibilidad al contraste y más concretamente en pacientes postmenopáusicas que presentan patología de "ojo seco". Por ello se plantea este estudio, cuyo objetivo es valorar la eficacia del hialuronato sódico en mujeres potmenopáusicas que sufren esta patología, mediante la determinación de las variaciones en su función de la sensibilidad al contraste.Item Eficacia bloqueante de filtros amarillos artificiales en procesos de degeneración retiniana(2005) Sánchez Ramos, Celia; Benítez Del Castillo Sánchez, José Manuel; Moral Martínez, M.I.; Sáenz Francés, F.; Álvarez, A.; Pérez Carrasco, María Jesús; Puell Marín, María Cinta; Langa Moraga, AntonioEl ojo dispone de un sistema de protección combinado que consiste evitar que las neuronas maculares absorban la luz visible azul. La protección retineana natural está sustentada por cromóforos producidos en el proceso de madurez y envejecimiento del cristalino y por pigmentos maculares: carotenoides- luteína y zeaxantina. Con la extracción del cristalino, en ojos operados de cataratas, se eliminan con la lente los cromóforos, dejando la retina expuesta a la acción de longitudes de onda corta que producen daños irreversibles. El propósito de este trabajo es comparar el efecto bloqueante de las sustancias amarillas naturales y de os filtros artificiales así como su papel protector en procesos evolutivos de degeneración macular asociada a la edad. La tomografía de coherencia óptica es una innovadora tecnología imprescindible para valorar la evolución de los procesos degenerativos del epitelio pigmentario, de la capa de neuronas ganglionares y de los fotorreceptores de la retina. En esta investigación se ha diseñado un estudio transversal y longitudinal de tres años de duración en ojos vivos viejos psudoafáquicos. Se realiza cirugía de catarata extracapsular con implantación de lente intraocular transparentes o con filtro amarillo en el saco capsular de conejos pigmentados mayores de dos años. Los animales erán expuestos a la cantidad variable de luz blanca y luz azul según el grupo. Para la evolución histológica se utilizará el instrumento de tomografía de coherencia óptica.Item Novel Osmoprotective DOPC-DMPC Liposomes Loaded with Antihypertensive Drugs as Potential Strategy for Glaucoma Treatment(Pharmaceutics, 2022) González-Cela Casamayor, Miriam Ana; López Cano, José Javier; Bravo Osuna, Irene; Andrés Guerrero, Vanessa; Vicario De La Torre, Marta; Guzmán Navarro, Manuel; Benítez Del Castillo Sánchez, José Manuel; Herrero Vanrell, María Del Rocío; Molina Martínez, Irene TeresaGlaucoma is a group of chronic irreversible neuropathies that affect the retina and the optic nerve. It is considered one of the leading causes of blindness in the world. Although it can be due to various causes, the most important modifiable risk factor is the elevated intraocular pressure (IOP). In this case, the treatment of choice consists of instilling antihypertensive formulations on the ocular surface. The chronicity of the pathology, together with the low bioavailability of the drugs that are applied on the ocular surface, make it necessary to instill the formulations very frequently, which is associated, in many cases, with the appearance of dry eye disease (DED). The objective of this work is the design of topical ocular formulations capable of treating glaucoma and, at the same time, preventing DED. For this, two liposome formulations, loaded with brimonidine or with travoprost, were Tadeveloped using synthetic phospholipids and enriched by the addition of compounds with osmoprotective activity. The proposed formulations not only presented physicochemical characteristics (size, pH, osmolarity, surface tension, and viscosity) and encapsulation efficiency values (EE% of 24.78% and ≥99.01% for brimonidine and travoprost, respectively) suitable for ocular surface administration, but also showed good tolerance in human corneal and conjunctival cell cultures, as well as an in vitro osmoprotective activity. The hypotensive effect of both liposomal formulations was evaluated in normotensive albino New Zealand rabbits, showing a faster and longer lasting reduction of intraocular pressure in comparison to the corresponding commercialized products used as control. According to these results, the hypotensive liposomal formulations combined with osmoprotective agents would result in a very promising platform for the treatment of glaucoma and the simultaneous protection of the ocular surface.Item Novel liposome-based and in situ gelling artificial tear formulation for dry eye disease treatment(Contact Lens & Anterior Eye, 2018) Acar, Duygu; Molina Martínez, Irene Teresa; Gómez Ballesteros, Miguel; Guzmán Navarro, Manuel; Benítez Del Castillo Sánchez, José Manuel; Herrero Vanrell, María Del RocíoPurpose. Artificial tears are widely used in the treatment of dry eye disease, although current formulations do not closely resemble natural tears. The purpose of this study was the design and characterization of a novel in situ gelling artificial tear formulation, containing both lipid and aqueous components, in order to resemble natural tears and replenish the tear film. Methods. Liposomes, containing phosphatidylcholine, cholesterol, vitamins A and E, were prepared by the thin-film hydration method. The aqueous phase of the formulation was comprised of gellan gum, hydroxypropyl methylcellulose, levocarnitine, electrolytes (sodium chloride and potassium chloride), trehalose, and borates. The artificial tear was characterized in terms of liposome size, pH, surface tension, and viscosity. In vitro tolerance studies were performed in a human epithelial carcinoma cell line (HeLa) and a murine macrophage cell line (J774). In vivo tolerance was assessed in rabbits. Results. Liposomes presented a unimodal distribution with a mean size of 200.1 ± 4.4 nm. The resulting surface tension was 53.4 ± 1.1 mN/m (at 33°C) and the pH was 7.6 ± 0.1. The viscosity of the formulation presented a mean value of 4.0 ± 0.1 mPa.s within the shear rate interval of 200-1000 s-1 at 33°C. Cell viability remained higher than 90% in both cell lines. No discomfort or clinical signs were observed in rabbits. Conclusions. The liposome-based and in situ gelling artificial tear formulation presented good tolerance and suitable properties for topical ophthalmic administration. It may be beneficial in the treatment of dry eye disease.Item Ultrasound biomicroscopy examination of posterior chamber phakic intraocular lens position(Ophthalmology, 2003) García Feijoo, Julián; Jiménez Alfaro, Iñigo; Cuiña Sardiña, Ricardo; Méndez Hernández, Carmen Dora; Benítez Del Castillo Sánchez, José Manuel; García Sánchez, JuliánObjective: To better elucidate the in vivo position of the Collamer posterior chamber phakic intraocular lens (PCPIOL) and its relationship to the iris and the crystalline lens and to analyze possible variations over time. Design: Prospective observational case series. Participants: Eighteen eyes of nine patients were included. Intervention: A Staar Collamer implantable PCPIOL was implanted for the correction of high myopia. Main Outcome Measures: The eyes were studied with a 50-MHz ultrasound biomicroscopy UBM 840. The exact PCPIOL position and the distances between it and the crystalline lens were measured at 3, 6, and 12 months after surgery. Results: There were no intraoperative complications. In 13 eyes (72.22%), contact between the PCPIOL and the crystalline lens was found at some time during follow-up. In 3 eyes (16.6%), central contact could be demonstrated. We also observed that the contact zone and its extension can vary over time. In 2 eyes, rotation of the lens was observed. Conclusions: We found contact between the PCPIOL and the crystalline lens in a high percentage of cases. There was also mobility of the lens in the posterior chamber, especially in the anteroposterior plane, and, as a consequence, both the contact zone and its extension would vary over time. © 2003 by the American Academy of Ophthalmology.