Person: Guerrero Martínez, Andrés
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First Name
Andrés
Last Name
Guerrero Martínez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Física
Area
Química Física
Identifiers
6 results
Search Results
Now showing 1 - 6 of 6
Item Intercellular Trafficking of Gold Nanostars in Uveal Melanoma Cells for Plasmonic Photothermal Therapy(Nanomaterials, 2020) Ahijado Guzmán, Rubén; Sánchez Arribas, Natalia; Martínez Negro, María; González Rubio, Guillermo; Santiago-Varela, María; Pardo, María; Piñeiro, Antonio; López-Montero, Iván; Junquera González, Elena; Guerrero Martínez, AndrésEfficient plasmonic photothermal therapies (PPTTs) using non-harmful pulse laser irradiation at the near-infrared (NIR) are a highly sought goal in nanomedicine. These therapies rely on the use of plasmonic nanostructures to kill cancer cells while minimizing the applied laser power density. Cancer cells have an unsettled capacity to uptake, retain, release, and re-uptake gold nanoparticles, thus offering enormous versatility for research. In this work, we have studied such cell capabilities for nanoparticle trafficking and its impact on the effect of photothermal treatments. As our model system, we chose uveal (eye) melanoma cells, since laser-assisted eye surgery is routinely used to treat glaucoma and cataracts, or vision correction in refractive surgery. As nanostructure, we selected gold nanostars (Au NSs) due to their high photothermal efficiency at the near-infrared (NIR) region of the electromagnetic spectrum. We first investigated the photothermal effect on the basis of the dilution of Au NSs induced by cell division. Using this approach, we obtained high PPTT efficiency after several cell division cycles at an initial low Au NS concentration (pM regime). Subsequently, we evaluated the photothermal effect on account of cell division upon mixing Au NS-loaded and non-loaded cells. Upon such mixing, we observed trafficking of Au NSs between loaded and non-loaded cells, thus achieving effective PPTT after several division cycles under low irradiation conditions (below the maximum permissible exposure threshold of skin). Our study reveals the ability of uveal melanoma cells to release and re-uptake Au NSs that maintain their plasmonic photothermal properties throughout several cell division cycles and re-uptake. This approach may be readily extrapolated to real tissue and even to treat in situ the eye tumor itself. We believe that our method can potentially be used as co-therapy to disperse plasmonic gold nanostructures across affected tissues, thus increasing the effectiveness of classic PPTT.Item Intracellular pH-Induced Tip-to-Tip Assembly of Gold Nanorods for Enhanced Plasmonic Photothermal Therapy(ACS Omega, 2016) Ahijado Guzmán, Rubén; Bañares Morcillo, Luis; Guerrero Martínez, Andrés; López Montero, Iván; Tardajos Rodríguez, Gloria María; González Rubio, Guillermo; Izquierdo, Jesús G.; Calzado Martín, Alicia; Calleja, MontserratThe search for efficient plasmonic photothermal therapies using nonharmful pulse laser irradiation at the near-infrared (NIR) is fundamental for biomedical cancer research. Therefore, the development of novel assembled plasmonic gold nanostructures with the aim of reducing the applied laser power density to a minimum through hot-spot-mediated cell photothermolysis is an ongoing challenge. We demonstrate that gold nanorods (Au NRs) functionalized at their tips with a pH-sensitive ligand assemble into oligomers within cell lysosomes through hydrogen-bonding attractive interactions. The unique intracellular features of the plasmonic oligomers allow us to significantly reduce the femtosecond laser power density and Au NR dose while still achieving excellent cell killing rates. The formation of gold tip-to-tip oligomers with longitudinal localized surface plasmon resonance bands at the NIR, obtained from low-aspect-ratio Au NRs close in resonance with 800 nm Ti:sapphire 90 fs laser pulses, was found to be the key parameter for realizing the enhanced plasmonic photothermal therapy.Item Microfluidic fabrication of vesicles with hybrid lipid/nanoparticle bilayer membranes(Soft Matter, 2019) Julie Perrotton; Ahijado Guzmán, Rubén; Lara H Moleiro; Berta Tinao; Guerrero Martínez, Andrés; Esther Amstad; Monroy Muñoz, Francisco; Laura R ArriagaHybrid lipid/nanoparticle membranes are suitable model systems both to study the complex interactions between nanoparticles and biological membranes, and to demonstrate technological concepts in cellular sensing and drug delivery. Unfortunately, embedding nanoparticles into the bilayer membrane of lipid vesicles is challenging due to the poor control over the vesicle fabrication process of conventional methodologies and the fragility of the modified lipid bilayer assembly. Here, the utility of water-in-oil-in-water double emulsion drops with ultrathin oil shells as templates to form vesicles with hybrid lipid/nanoparticle membranes is reported.Item Supramolecular zippers elicit interbilayer adhesion of membranes producing cell death(Biochimica et Biophysica Acta (BBA) - General Subjects, 2018) Almendro Vedia, Víctor Galileo; García, Carolina; Ahijado Guzmán, Rubén; de la Fuente Herreruela, Diego; Muñoz Úbeda, Mónica; Natale, Paolo; Viñas, Montserrat H.; Albuquerque, Rodrigo Queiroz; Guerrero Martínez, Andrés; Monroy, Francisco; Lillo, M. Pilar; López-Montero, IvánBackground: The fluorescent dye 10-N-nonyl acridine orange (NAO) is widely used as a mitochondrial marker. NAO was reported to have cytotoxic effects in cultured eukaryotic cells when incubated at high concentrations. Although the biochemical response of NAO-induced toxicity has been well identified, the underlying molecular mechanism has not yet been explored in detail. Methods: We use optical techniques, including fluorescence confocal microscopy and lifetime imaging microscopy (FLIM) both in model membranes built up as giant unilamellar vesicles (GUVs) and cultured cells. These experiments are complemented with computational studies to unravel the molecular mechanism that makes NAO cytotoxic. Results: We have obtained direct evidence that NAO promotes strong membrane adhesion of negatively charged vesicles. The attractive forces are derived from van der Waals interactions between anti-parallel H-dimers of NAO molecules from opposing bilayers. Semi-empirical calculations have confirmed the supramolecular scenario by which anti-parallel NAO molecules form a zipper of bonds at the contact region. The membrane remodeling effect of NAO, as well as the formation of H-dimers, was also confirmed in cultured fibroblasts, as shown by the ultrastructure alteration of the mitochondrial cristae. Conclusions: We conclude that membrane adhesion induced by NAO stacking accounts for the supramolecular basis of its cytotoxicity. General significance: Mitochondria are a potential target for cancer and gene therapies. The alteration of the mitochondrial structure by membrane remodeling agents able to form supramolecular assemblies via adhesion properties could be envisaged as a new therapeutic strategy.Item Tunable gold nanorod/NAO conjugates for selective drug delivery in mitochondria-targeted cancer therapy(Nanoscale, 2022) González-Rubio, Sergio; Salgado, Cástor; Manzaneda González, Vanesa; Muñoz Úbeda, Mónica; Ahijado Guzmán, Rubén; Natale, Paolo; Almendro Vedia, Víctor Galileo; Junquera González, María Elena; Osío Barcina, José De Jesús; Ferrer, Irene; Guerrero Martínez, Andrés; Paz-Ares Rodríguez, Luis Gonzaga; López Montero, Iván- Project number: PIMCD378/23-24
Item Innovación sobre la docencia y evaluación del laboratorio de Química Física I(2024) Omiste Romero, Juan José; Ahijado Guzmán, Rubén; Blázquez Fernández, Samuel; Caselli, Niccolo; Díaz Blanco, Cristina; Guerrero Martínez, Andrés; Hernández Díaz, María Yolanda; Labrador Páez, Lucía; Marggi Poullaín, Sonia; Pulido Lamas, Cintia; Sánchez Benítez, Francisco Javier; Sola Reija, Ignacio; Suardíaz Delrío, Reynier; Izquierdo Ruiz, Fernando; Lobato Fernández, Álvaro; Sánchez González, Julia