Person:
Hurtado Moreno, Olivia

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First Name
Olivia
Last Name
Hurtado Moreno
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Farmacología y Toxicología
Area
Farmacología
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UCM identifierScopus Author IDDialnet IDGoogle Scholar ID

Search Results

Now showing 1 - 10 of 18
  • Publication
    Perspectiva de género en la asignatura de farmacología
    Acevedo Couto, Érika del Carmen; Cuartero Desviant, María Isabel; García Culebras, Alicia; Hernández Jiménez, Macarena; Moraga Yébenes, Ana; Ortego Merino, Fernando; Pradillo Justo, Jesús Miguel; Sánchez Fernández, Marina; Hurtado Moreno, Olivia; Gallego Matud, Julieta; Sánchez Mayordomo, Andrea
    En este proyecto de innovación docente hemos trabajo para comenzar a poner las bases de posibles diferencias que pueden existir en la farmacología de los medicamentos, atendiendo a si la persona que los toma es un hombre o una mujer. Con alumnos del grado de Nutrición Humana y Dietética y de Odontología hemos buscado bibliografía que nos demuestre si efectivamente se dan estas diferencias en la farmacocinética, en la farmacodinamia y en los efectos adversos. Los resultados encontrados nos han permitido presentar 2 comunicaciones en congresos y realizar un TFG
  • Publication
    Enzymatic production of sustainable jojoba fatty acohols: A Biorefinery approach
    (Elsevier, 2023-03-13) Bouaid Bouaid, Abderrahim; Hurtado Moreno, Olivia; Tardón de la Flor, Natalia; Marchetti, Jorge Mario
    To evaluate jojoba oil (JO) in high-added-value products, a new biorefinery strategy was used, consisting of enzymatic transesterification employing a branched 2-ethylhexanol (EH) chain alcohol. The suggested biorefinery approach utilizes an integrated process to produce jojobyl alcohols (JAs) mixture (11-eicosenol, 13-docosenol, and 15-tetracosenol) as the principal product for pharmaceutical applications. Since it meets the European Biodiesel Standard EN 14214 in all tested aspect with the exemption of the viscosity, the remaining fraction of fatty acid ethyl-hexyl esters (FAEHEs) could be a potential alternative to traditional fuels. Factorial design (FD) and response surface methodology (RSM) were used to investigate and improve the effects of variables such as temperature and catalyst concentration on the production of both fractions. The derived models can be used to estimate the best operating parameters for an up-scaled industrial process with the least number of tests possible, resulting in cost savings. However, from a technical perspective, the best feasible yield for the more valuable JAs fraction should be achieved, using a catalyst concentration of 5.7%, a temperature of 63 °C, and a 6:1 alcohol/oil molar ratio. Conversion rates of 63.5% and 36% for JAs and FAEHEs, respectively, could be produced under these conditions. The crystallization method was used to separate JAs from FAEHEs. The tetrazolium dye reduction (MTT) test was used to assess in vitro cell viability in HEK293T cells. The findings revealed that a 1 μmolL−1 oily liquid mixture of jojobyl alcohols components (cis-11-eicosenol, cis-13-docosenol, and cis-15-tetracosenol) had no influence on cell cycle progression, has no harmful impacts in the examined cells, and could be employed as a therapeutic compound. The product preparation is a green engineering process that is clean, solvent-free, and uses a highly selective catalyst to reduce water and energy utilization, as well as the integrated process's downstream processing.
  • Publication
    Sustainable production of jojobyl alcohols and cell viability study
    (Wiley, 2021-06-10) Hurtado Moreno, Olivia; Bouaid Bouaid, Abderrahim; Acherki, Hassan
    Abstract: An innovative biorefinery approach has been applied to evaluate jojoba oil (JO) in high value-added products. It consists of enzymatic transesterification using short (1-propanol, 1-butanol), medium linear (1-octanol), and branched (2-ethylhexanol) chain alcohols. The proposed biorefinery approach uses an integrated process for the production of jojobyl alcohol (JA) mixtures (11-eicosenol, 13-docosenol and 15-tetracosenol) as products that can be used in pharmaceutical applications. The remaining fraction of fatty acid alkyl esters (FAAE) has a wide range of industrial uses and could be used as a promising alternative to conventional fuels, as it complies with EN14214, the European Biodiesel Norm. The separation of JA from FAAE was carried out by crystallization using a binary mixture of hexane and diethyl ether. After the separation step, the main properties of FAAE were determined. The results showed that the cold flow properties and oxidation stability of the FAAE fraction, obtained as co-product during the process, have been improved with respect to transesterified JO. The use of 2-ethylhexanol as an alcohol in the transesterification reaction increases the amount of the most valuable fraction containing JAs. In vitro cell viability was measured in HEK293T cells using the tetrazolium dye reduction (MTT) assay. The results showed that this oily liquid mixture of JA components (cis-11-eicosenol, cis-13-docosenol, and cis-15-tetracosenol) had a cytotoxic effect at concentrations of 10 and 100 μmol L−1 and no cytotoxic activity at 1 μmol L−1. The concentration of 1 μmol L−1 does not, therefore, modify the cell viability, does not produce toxic effects in the tested cells and could be used as a therapeutic compound.
  • Publication
    Papel de la convertasa de TNF-[alfa] en modelos de isquemia cerebral
    (Universidad Complutense de Madrid, Servicio de Publicaciones, 2004) Hurtado Moreno, Olivia; Moro Sánchez, María Ángeles; Lizasoain Hernández, Ignacio
    Mediante el uso de dos modelos in vitro de isquemia cerebral, que son las rebanadas de cerebro anterior de rata y el cultivo primario de neuronas corticales hemos estudiado lo siguiente: * Si existe liberación de TNF-alfa tras una isquemia experimental. * Si este TNF-alfa liberado procede del enzima TACE * Que ocurre con la expresión y actividad TACE después de una isquemia experimental. * Cuál es el papel del glutámico en la expresión de TACE. * Estudiar las acciones tóxicas y neuroprotectoras del TNF-alfa
  • Publication
    Propuesta de mejora de la asignatura TFG del Grado en Nutrición Humana y Diétetica. Implantación de la prueba ECOE
    (2019-05-28) Hurtado Moreno, Olivia; Calle Purón, María Elisa; Cuadrado Vives, María Carmen; Morales Gómez, Paloma; Lafuente Duarte, María Esther; Hierro Paredes, Eva; López Gallardo, Meritxell; Redondo Cuenca, Araceli; Villarino Marín, Antonio Luis; Matallana González, María Cruz; Calle Pascual, Alfonso Luis
  • Publication
    Toll-like receptor 4 regulates subventricular zone proliferation and neuroblast migration after experimental stroke
    (Elsevier, 2019) Palma-Tortosa, Sara; Ferreras-Martín, Raquel; García-Yébenes, Isaac; Moraga, Ana; Hurtado Moreno, Olivia; Pradillo Justo, Jesús Miguel; García Culebras, Alicia; Moro Sánchez, María Ángeles; Lizasoaín Hernández, Ignacio
    Ischemic stroke is one of the leading causes of death and disability with an urgent need for innovative therapies, especially targeting the chronic phase. New evidence has emerged showing that Toll-Like Receptor 4 (TLR4), a key mediator of brain damage after stroke, may be involved in brain repair by neurogenesis modulation. The aim of this study is to analyze the role of TLR4 in the different stages of neurogenesis initiated in the subventricular zone (SVZ) over time after stroke in mice. Wildtype and TLR4-deficient mice underwent experimental ischemia, and neural stem/progenitor cells (NSPCs) proliferation and migration were analyzed by using FACS analysis, fluorescence densitometry, RT-qPCR and in vitro assays. Our results show that both groups, wildtype and knock-out animals, present a similar pattern of bilateral cell proliferation at the SVZ, with a decrease in NSPCs pro-liferation in the acute phase of stroke. We also show that TLR4 activation, very likely mediated by ligands such as HMGB1 released to CSF after stroke, is necessary to keep an increased proliferation of NSCs as well as to promote differentiation from type C cells into neuroblasts promoting their migration. TLR4 activation was also implicated in earlier expression of SDF-1α and faster recovery of BDNF expression after stroke. These results support TLR4 as an important therapeutic target in the modulation of neurogenesis after stroke.
  • Publication
    Gestión de exámenes online. Base de datos de preguntas y desarrollo de software
    (2021-06-28) Pérez Vizcaíno, Francisco; Esquivel Ruiz, Sergio Antonio; Gil De Biedma Elduayen, Leticia; Macías Montero, Miguel; Morales Puerto, Nuria; Núñez De La Calle, Carlos; Olivencia Plaza, Miguel Ángel; Bas Caro, Manuel; Caso Fernandez, Javier Rubén; Cogolludo Torralba, Ángel Luis; García Bueno, Borja; Gutiérrez Lopez, María Dolores; Hurtado Moreno, Olivia; Leza Cerro, Juan Carlos; Moreno Gutiérrez, Laura; O'Shea Gaya, María Esther; Pradillo Justo, Jesús Miguel; Vidal Casado, Rebeca
  • Publication
    Defective hippocampal neurogenesis underlies cognitive impairment by carotid stenosis-induced cerebral hypoperfusion in mice
    (2023-08-11) Fraga, Enrique; Medina, Violeta; Cuartero Desviat, María Isabel; García Culebras, Alicia; Bravo Ferrer, Isabel; Hernández Jiménez, Macarena; García Segura, Juan Manuel; Hurtado Moreno, Olivia; Pradillo Justo, Jesús Miguel; Lizasoaín Hernández, Ignacio; Moro Sánchez, María Ángeles
    Chronic cerebral hypoperfusion due to carotid artery stenosis is a major cause of vascular cognitive impairment and dementia (VCID). Bilateral carotid artery stenosis (BCAS) in rodents is a well-established model of VCID where most studies have focused on white matter pathology and subsequent cognitive deficit. Therefore, our aim was to study the implication of adult hippocampal neurogenesis in hypoperfusion-induced VCID in mice, and its relationship with cognitive hippocampal deficits. Mice were subjected to BCAS; 1 and 3 months later, hippocampal memory and neurogenesis/cell death were assessed, respectively, by the novel object location (NOL) and spontaneous alternation performance (SAP) tests and by immunohistology. Hypoperfusion was assessed by arterial spin labeling-magnetic resonance imaging (ASL-MRI). Hypoperfused mice displayed spatial memory deficits with decreased NOL recognition index. Along with the cognitive deficit, a reduced number of newborn neurons and their aberrant morphology indicated a remarkable impairment of the hippocampal neurogenesis. Both increased cell death in the subgranular zone (SGZ) and reduced neuroblast proliferation rate may account for newborn neurons number reduction. Our data demonstrate quantitative and qualitative impairment of adult hippocampal neurogenesis disturbances associated with cerebral hypoperfusion-cognitive deficits in mice. These findings pave the way for novel diagnostic and therapeutic targets for VCID.
  • Publication
    Cannabinoid Type-2 Receptor Drives Neurogenesis and Improves Functional Outcome After Stroke
    (American Heart Association, 2017-01) Bravo Ferrer, Isbel; Cuartero Desviat, María Isabel; Zarruk, Juan G.; Pradillo, Jesús M.; Hurtado Moreno, Olivia; Romera, Victor G.; Díaz Alonso, Javier; García Segura, Juan Manuel; Guzmán, Manuel; Lizasoaín, Ignacio; Galve Roperh, Ismael; Moro, María A.
    Background and Purpose—Stroke is a leading cause of adult disability characterized by physical, cognitive, and emotional disturbances. Unfortunately, pharmacological options are scarce. The cannabinoid type-2 receptor (CB2R) is neuroprotective in acute experimental stroke by anti-inflammatory mechanisms. However, its role in chronic stroke is still unknown. Methods—Stroke was induced by permanent middle cerebral artery occlusion in mice; CB2R modulation was assessed by administering the CB2R agonist JWH133 ((6aR,10aR)-3-(1,1-dimethylbutyl)-6a,7,10,10a-tetrahydro-6,6,9-trimethyl-6Hdibenzo[b,d]pyran) or the CB2R antagonist SR144528 (N-[(1S)-endo-1,3,3-trimethylbicyclo-[2.2.1]-heptan-2-yl]-5-(4- chloro-3-methylphenyl)-1-(4-methylbenzyl)-pyrazole-3-carboxamide) once daily from day 3 to the end of the experiment or by CB2R genetic deletion. Analysis of immunofluorescence-labeled brain sections, 5-bromo-2´-deoxyuridine (BrdU) staining, fluorescence-activated cell sorter analysis of brain cell suspensions, and behavioral tests were performed. Results—SR144528 decreased neuroblast migration toward the boundary of the infarct area when compared with vehicletreated mice 14 days after middle cerebral artery occlusion. Consistently, mice on this pharmacological treatment, like mice with CB2R genetic deletion, displayed a lower number of new neurons (NeuN+ /BrdU+ cells) in peri-infarct cortex 28 days after stroke when compared with vehicle-treated group, an effect accompanied by a worse sensorimotor performance in behavioral tests. The CB2R agonist did not affect neurogenesis or outcome in vivo, but increased the migration of neural progenitor cells in vitro; the CB2R antagonist alone did not affect in vitro migration. Conclusions—Our data support that CB2R is fundamental for driving neuroblast migration and suggest that an endocannabinoid tone is required for poststroke neurogenesis by promoting neuroblast migration toward the injured brain tissue, increasing the number of new cortical neurons and, conceivably, enhancing motor functional recovery after stroke.
  • Publication
    Base de datos de preguntas y actualización del software. Herramientas para la generación de exámenes presenciales u online
    (2022-09-30) O'Shea Gaya, María Esther; Pérez Vizcaíno, Francisco; Caso Fernández, Javier Rubén; Cogolludo Torralba, Ángel Luis; García Bueno, Borja; Gutiérrez López, María Dolores; Hurtado Moreno, Olivia; Leza Cerro, Juan Carlos; Moreno Gutiérrez, Laura; Pradillo Justo, Jesús Miguel; Vidal Casado, Rebeca; Esquivel Ruiz, Sergio Antonio; Gil De Biedma Elduayen, Leticia; Macías Montero, Miguel; Morales Puerto, Nuria; Núñez De La Calle, Carlos; Olivencia Plaza, Miguel Ángel; Bas Caro, Manuel; Morales Cano, Daniel