Person: Rodilla González, Beatriz Loreto
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First Name
Beatriz Loreto
Last Name
Rodilla González
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Físicas
Department
Física de Materiales
Area
Identifiers
4 results
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Item Nanostructured gold electrodes promote neural maturation and network connectivity(Biomaterials, 2021) Dominguez-Bajo, Ana; Rosa, Juliana M.; González-Mayorga, Ankor; Rodilla González, Beatriz Loreto; Arché-Nuñez, Ana; Benayas, Esther; Ocón, Pilar; Pérez García, Lucas; Camarero, Julio; Miranda, Rodolfo; González, M. Teresa; Aguilar, Juan; Lopez-Dolado, Elisa; Serrano, María C.Progress in the clinical application of recording and stimulation devices for neural diseases is still limited, mainly because of suboptimal material engineering and unfavorable interactions with biological entities. Nanotechnology is providing upgraded designs of materials to better mimic the native extracellular environment and attain more intimate contacts with individual neurons, besides allowing for the miniaturization of the electrodes. However, little progress has been done to date on the understanding of the biological impact that such neural interfaces have on neural network maturation and functionality. In this work, we elucidate the effect of a gold (Au) highly ordered nanostructure on the morphological and functional interactions with neural cells and tissues. Alumina-templated Au nanostructured electrodes composed of parallel nanowires of 160 nm in diameter and 1.2 mu m in length (Au-NWs), with 320 nm of pitch, are designed and characterized. Equivalent non-structured Au electrodes (Au-Flat) are used for comparison. By using diverse techniques in in vitro cell cultures including live calcium imaging, we found that Au-NWs interfaced with primary neural cortical cells for up to 14 days allow neural networks growth and increase spontaneous activity and ability of neuronal synchronization, thus indicating that nanostructured features favor neuronal network. The enhancement in the number of glial cells found is hypothesized to be behind these beneficial functional effects. The in vivo effect of the implantation of these nanostructured electrodes and its potential relevance for future clinical applicability has been explored in an experimental model of rat spinal cord injury. Subacute responses to implanted Au-NWs show no overt reactive or toxic biological reactions besides those triggered by the injury itself. These results highlight the translational potential of Au-NWs electrodes for in vivo applications as neural interfaces in contact with central nervous tissues including the injured spinal cord.Item Flexible metallic core–shell nanostructured electrodes for neural interfacing(Scientific Reports, 2024) Rodilla González, Beatriz Loreto; Arché-Nuñez, Ana; Ruiz-Gómez, Sandra; Domínguez-Bajo, Ana; Fernández-González, Claudia; Guillén-Colomer, Clara; González-Mayorga, Ankor; Rodríguez-Díez, Noelia; Camarero, Julio; Miranda, Rodolfo; López-Dolado, Elisa; Ocón, Pilar; Serrano, María Concepción; Pérez García, Lucas; González, M. TeresaElectrodes with nanostructured surface have emerged as promising low-impedance neural interfaces that can avoid the charge‐injection restrictions typically associated to microelectrodes. In this work, we propose a novel approximation, based on a two-step template assisted electrodeposition technique, to obtain flexible nanostructured electrodes coated with core–shell Ni–Au vertical nanowires. These nanowires benefit from biocompatibility of the Au shell exposed to the environment and the mechanical properties of Ni that allow for nanowires longer and more homogeneous in length than their only-Au counterparts. The nanostructured electrodes show impedance values, measured by electrochemical impedance spectroscopy (EIS), at least 9 times lower than those of flat reference electrodes. This ratio is in good accordance with the increased effective surface area determined both from SEM images and cyclic voltammetry measurements, evidencing that only Au is exposed to the medium. The observed EIS profile evolution of Ni–Au electrodes over 7 days were very close to those of Au electrodes and differently from Ni ones. Finally, the morphology, viability and neuronal differentiation of rat embryonic cortical cells cultured on Ni–Au NW electrodes were found to besimilar to those on control (glass) substrates and Au NW electrodes, accompanied by a lower glial cell differentiation. This positive in-vitro neural cell behavior encourages further investigation to explore the tissue responses that the implantation of these nanostructured electrodes might elicit in healthy (damaged) neural tissues in vivo, with special emphasis on eventual tissue encapsulation.Item Electrodeposited magnetic nanowires with radial modulation of composition(Nanomaterials, 2022) Fernández González, Claudia; Guedeja-Marrón Gil, Alejandra; Rodilla González, Beatriz Loreto; Arché Nuñez, Ana; Corcuera, Rubén; Lucas, Irene; González, María Teresa; Varela del Arco, María; Presa Muñoz del Toro, Patricia de la; Aballe, Lucía; Pérez García, Lucas; Ruíz Gómez, SandraIn the last few years, magnetic nanowires have gained attention due to their potential implementation as building blocks in spintronics applications and, in particular, in domain-wall- based devices. In these devices, the control of the magnetic properties is a must. Cylindrical magnetic nanowires can be synthesized rather easily by electrodeposition and the control of their magnetic properties can be achieved by modulating the composition of the nanowire along the axial direction. In this work, we report the possibility of introducing changes in the composition along the radial direction, increasing the degrees of freedom to harness the magnetization. In particular, we report the synthesis, using template-assisted deposition, of FeNi (or Co) magnetic nanowires, coated with a Au/Co (Au/FeNi) bilayer. The diameter of the nanowire as well as the thickness of both layers can be tuned at will. In addition to a detailed structural characterization, we report a preliminary study on the magnetic properties, establishing the role of each layer in the global collective behavior of the system.Item Fabrication and characterization of nanostructured electrodes for more efficient low‐invasiveness neural interfaces(2022) Rodilla González, Beatriz Loreto; Pérez García, Lucas; González Pérez, María TeresaNeurological disorders produce serious cognitive and motor disabilities, and they account for 7% of total global disease burden, measured in disability‐adjusted life years. In addition, the life expectancy has been continuously growing during the last decades and, as a consequence, neurodegenerative disorders are becoming more prevalent representing a larger part of the healthcare efforts and expenses. Every year, treating brain conditions accounts for 35% of Europe’s disease burden with a yearly cost of €798.000 million. Despite the efforts performed in the medical and science fields, the cure for most of the neurological disorders is far from being achieved. One of the most efficient strategies to treat neurological disorders is the use of implanted electrodes to produce neural electrical stimulation. Furthermore, electrodes are one of the main neural interfaces used in diagnostics techniques, and for the study of the neural activity in basic investigation, in vitro and in vivo. Despite their enormous potential, these electrodes face nowadays limitations. Generally, they are too big, producing unspecific stimulation that can lead to secondary effects. Their size reduction is limited by the associated impedance increase, which restricts their charge‐injection to the tissue...