Person:
García Linares, Sara

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First Name
Sara
Last Name
García Linares
URI
https://hdl.handle.net/20.500.14352/85452
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Bioquímica y Biología Molecular
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2020 - 20226
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Now showing 1 - 6 of 6
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    Structural foundations of sticholysin functionality
    (BBA - Proteins and Proteomics, 2021) Palacios Ortega, Juan; García Linares, Sara; Rivera de Torre, Esperanza; Heras Márquez, Diego; Gavilanes, José G.; Peter, Slotte; Martínez del Pozo, Álvaro
    Actinoporins constitute a family of α pore-forming toxins produced by sea anemones. The soluble fold of these proteins consists of a β-sandwich flanked by two α-helices. Actinoporins exert their activity by specifically recognizing sphingomyelin at their target membranes. Once there, they penetrate the membrane with their N-terminal α-helices, a process that leads to the formation of cation-selective pores. These pores kill the target cells by provoking an osmotic shock on them. In this review, we examine the role and relevance of the structural features of actinoporins, down to the residue level. We look at the specific amino acids that play significant roles in the function of actinoporins and their fold. Particular emphasis is given to those residues that display a high degree of conservation across the actinoporin sequences known to date. In light of the latest findings in the field, the membrane requirements for pore formation, the effect of lipid composition, and the process of pore formation are also discussed.
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    Project number: 350
    EChemTest: sistema de evaluación de la Calidad en Química
    (2022) Sánchez Benítez, Francisco Javier; Díaz Blanco, Cristina; Guerrero Martínez, Andrés; Gutiérrez Alonso, Ángel; Lacadena García-Gallo, Francisco Javier; Lainez Ferrando, Alfredo; Pilo Santos, Miguel; Villalba Díaz, MaríaTeresa; García Linares, Sara
    Este proyecto plantea la herramienta EChemTest como mecanismo de evaluación de la Calidad de un Grado relacionado con la Química. También presenta la oportunidad de evaluar cómo ha influido la docencia online en la adquisición de conocimientos, comparando con cursos anteriores.
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    Jornadas sobre la carrera investigadora: Módulo I, primera sesión: Etapas de la carrera Investigadora (predoctoral y postdoctoral).
    (2020) Martínez Rodrigo, Abel; Salazar Roa, María; Valdés Mora, Fátima; García Linares, Sara; Arias Álvarez, María; Briones Dieste, Víctor; San Andrés Moya, Margarita; Varela Nieto, Isabel; Serres Dalmau, María Consolacion; Gascón Inchausti, Fernando
    La Facultad de Veterinaria y la Sociedad Española de Bioquímica y Biología Molecular (SEBBM) organizan de manera conjunta unas I Jornadas sobre la carrera investigadora que comenzarán en Enero de 2021. En la organización se ha colaborado de manera conjunta con otras Facultades de la UCM con similitud en sus programas de doctorado como son la Facultad de CC. Biológicas, la Facultad de CC. Químicas, la Facultad de Medicina y la Facultad de Farmacia. Las jornadas están encuadradas dentro de las Actividades Formativas de la Escuela de Doctorado de la UCM y están orientadas principalmente a Estudiantes de Doctorado, pero también a Investigadores en distintas etapas de su carrera investigadora, en el área de Ciencias de la Salud y Experimentales principalmente. Asimismo, hay sesiones que pueden resultar de interés para otras disciplinas debido a su carácter transversal.
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    Functional and Structural Variation among Sticholysins, Pore-Forming Proteins from the Sea Anemone Stichodactyla helianthus
    (International journal of molecular sciences, 2020) Rivera de la Torre, Esperanza; Palacios Ortega, Juan; Slotte, J. Peter; Gavilanes, José G.; Martínez del Pozo, Álvaro; García Linares, Sara
    Venoms constitute complex mixtures of many different molecules arising from evolution in processes driven by continuous prey–predator interactions. One of the most common compounds in these venomous cocktails are pore-forming proteins, a family of toxins whose activity relies on the disruption of the plasmatic membranes by forming pores. The venom of sea anemones, belonging to the oldest lineage of venomous animals, contains a large amount of a characteristic group of pore-forming proteins known as actinoporins. They bind specifically to sphingomyelin-containing membranes and suffer a conformational metamorphosis that drives them to make pores. This event usually leads cells to death by osmotic shock. Sticholysins are the actinoporins produced by Stichodactyla helianthus. Three different isotoxins are known: Sticholysins I, II, and III. They share very similar amino acid sequence and three-dimensional structure but display different behavior in terms of lytic activity and ability to interact with cholesterol, an important lipid component of vertebrate membranes. In addition, sticholysins can act in synergy when exerting their toxin action. The subtle, but important, molecular nuances that explain their different behavior are described and discussed throughout the text. Improving our knowledge about sticholysins behavior is important for eventually developing them into biotechnological tools.
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    Sticholysin I–II oligomerization in the absence of membranes
    (FEBS Letters, 2022) García Linares, Sara; Amigot Sánchez, Rafael; García Montoya, Carmen; Alfonso, Carlos; Luque Ortega, Juan Román; Gavilanes, José G.; Martínez del Pozo, Álvaro; Palacios Ortega, Juan
    Sticholysins are pore-forming toxins produced by the sea anemone Stichodactyla helianthus. When they encounter a sphingomyelin-containing membrane, these proteins bind to it and oligomerize, a process that ends in pore formation. Mounting evidence indicates that StnII can favour the activity of StnI. Previous results have shown that these two isotoxins can oligomerize together. Furthermore, StnII appeared to potentiate the activity of StnI through the membrane-binding step of the process. Hence, isotoxin interaction should occur prior to membrane encounter. Here, we have used analytical ultracentrifugation to investigate the oligomerization of Stns in solution, both separately and together. Our results indicate that while StnI seems to be more prone to oligomerize in water solution than StnII, a small percentage of StnII in StnI–StnII mixtures promotes oligomerization.
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    Project number: 34
    La conciencia social es la vacuna
    (2021) García Linares, Sara; Ferrari Puerta, Alberto; Rivera de Torre, Esperanza; Castillo Sánchez, José Carlos