Person: Martín Espada, María Del Carmen
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First Name
María Del Carmen
Last Name
Martín Espada
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
Identifiers
8 results
Search Results
Now showing 1 - 8 of 8
Item In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species(Mycoses, 2013) Carrillo‐Muñoz, Alfonso Javier; Rojas, Florencia; Tur‐Tur, Cristina; Sosa, María de los Ángeles; Ortiz Díez, Gustavo; Martín Espada, María Del Carmen; Paya Vicens, María Jesús; Giusiano, GustavoThe strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27-A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more-susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.case">Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.Item Producción de fosfolipasa y proteinasa en cepas de Malassezia pachydermatis aisladas de perros con otitis y sin otitis(Revista Iberoamericana de Micología, 2013) Ortiz Díez, Gustavo; Martín Espada, María Del Carmen; Carrillo Muñoz, Alfonso J.; Paya Vicens, María JesúsBackground: Malassezia pachydermatis is part of the skin microbiota of dogs and cats. M. pachydermatis has been associated with external otitis and seborrhoeic dermatitis, reported more often in dogs than in cats. When the physical, chemical or immunological mechanisms of the skin are altered, M. pachydermatis could act as a pathogen. Thus, several virulence factors, such as the ability to produce esterase, lipase, lipoxygenase, protease, chondroitin sulphatase, and hyaluronidase, have been studied. Aims: In the present study, we aim to identify the phospholipase activity measured at pH 6.3, and the proteinase activity measured at pH 6.3 and pH 6.8 (pH from ears of dogs with external otitis) of M. pachydermatis strains isolated from dogs with and without external otitis. Methods: The phospholipase activity was measured using a semi-quantitative method with egg yolk, and the proteinase activity with a semi-quantitative method using bovine serum albumin agar. The study was performed on 96 isolates of M. pachydermatis, 43 isolated from dogs without clinical symptoms of otitis, and 52 isolated from dogs with otitis. Results: In our study, 75.8% of the isolates showed phospholipase activity at pH 6.3, and 81 and 97.9% of them showed proteinase activity measured at pH 6.3 and 6.8, respectively. A higher phospholipase activity was detected in strains isolated from dogs with otitis. The proteinase activity was increased at a pH of 6.8 (97.9%) in comparison to a pH of 6.3 (81%). Conclusions: Our results suggest that the phospholipase activity may play an important role in the invasion of host tissues in chronic canine otitis cases. The proteinase activity results obtained in this study suggest that a reduction in the pH of the treatment may improve its efficacy in the resolution of M. pachydermatis otitis.Item Klebsiella pneumoniae sequence type 11 from companion animals bearing ArmA methyltransferase, DHA-1 β-lactamase, and QnrB4(Antimicrobial agents and chemotherapy, 2013) Hidalgo, Laura; Gutiérrez, Belén; Martínez Ovejero, Cristina; Carrilero Aguado, Laura; Matrat, Stephanie; Saba, Courage K S; Santos López, Alfonso; Thomas López, Daniel; Hoefer, Andreas; Suárez Rodríguez, Mónica; Santurde Sánchez, Gloria; Martín Espada, María Del Carmen; González Zorn, BrunoSeven Klebsiella pneumoniae isolates from dogs and cats in Spain were found to be highly resistant to aminoglycosides, and ArmA methyltransferase was responsible for this phenotype. All isolates were typed by multilocus sequence typing (MLST) as ST11, a human epidemic clone reported worldwide and associated with, among others, OXA-48 and NDM carbapenemases. In the seven strains, armA was borne by an IncR plasmid, pB1025, of 50 kb. The isolates were found to coproduce DHA-1 and SHV-11 β-lactamases, as well as the QnrB4 resistance determinant. This first report of the ArmA methyltransferase in pets illustrates their importance as a reservoir for human multidrug-resistant K. pneumoniae.- Project number: 106
Item Elaboración de una galería fotográfica de microorganismos aislados de procesos patológicos animales(2019) García Sánchez, Marta Eulalia; Martín Espada, María Del Carmen; Santos Ocaña, Sandra; Neila Mota, Carlos; Blanco Cancelo, José LuisSe ha elaborado una galería de fotografías a partir de 77 casos clínicos diferentes. Dentro de cada uno de ellos se dispone tanto de fotos macroscópicas como microscópicas, hasta un total de 383 fotografías. - Project number: 96
Item Atlas QR de procedimientos básicos en clínica hospitalaria equina(2018) López San Román, Francisco Javier; Fores Jackson, Paloma; Manso Díaz, Gabriel; Varela Del Arco, Marta; Troya Portillo, Lucas; Santiago Llorente, Isabel; Martín Espada, María Del Carmen; Rocha Lopesino, Luis Alberto de la; López Abradelo, PatriciaLos códigos QR se utilizan actualmente como herramienta docente de gran utilidad ya que permiten al alumno emplear las nuevas tecnologías para el aprendizaje. Con este proyecto pretendemos acercar la actividad clínica equina al alumno de veterinaria. Item Highly Tigecycline-Resistant Klebsiella pneumoniae Sequence Type 11 (ST11) and ST147 Isolates from Companion Animals(Antimicrobial Agents and Chemotherapy, 2017) Martinez Ovejero, Cristina; Thomas-Lopez, Daniel; Hoefer, Andreas; Moyano, Gabriel; Escudero García-Calderón, José Antonio; González Zorn, Bruno; Martín Espada, María Del Carmen; Montero Serra, NataliaIn this study, we characterized two tigecycline-resistant Klebsiella pneumoniae isolates from dog urine samples. The isolates were genetically unrelated, belonging to sequence type 11 (ST11) and ST147, both classically related to human isolates. To the best of our knowledge, this is the first identification of tigecycline-resistant isolates from animals. We unveil here the worrisome circulation among animals of bacterial clones resistant to this last-resort antibiotic.Item Description of postoperative complications and bacterial contamination of wound soaker catheters used to administer postoperative local analgesia after mastectomy in 11 dogs: case series(Veterinary Research Communications, 2024) Suárez Redondo, María; Fuertes Recuero, Manuel; Guzmán Soltero, Alba; Aguado Domínguez, Delia; Martín Espada, María Del Carmen; Espinel Rupérez, Jorge; Ortiz Díez, GustavoMastectomy is a common and painful procedure in dogs. Wound soaker catheters (WSC) are frequently used to reduce postoperative pain, including pain after mastectomy. The objectives of this case series were to describe the use of WSC for owner administration of postoperative local analgesia in dogs with mammary tumors treated surgically, to identify complications associated with WSC and to determine the frequency of bacterial colonization of the catheters. Twelve WSC were placed in 11 dogs during mastectomy surgery, left in place for three days, protected by a dressing and successfully managed by owners at home. No postoperative antibiotics were administered. No complications were identified in any cases. No bacterial growth was identified on bacteriological analysis of the twelve WSC. These results suggest that the use of WSC is a safe alternative for postoperative analgesia administration following mastectomy in dogs. Future studies comparing dogs with or without WSC with a larger number of dogs are needed to further evaluate efficacy and complications.Item Sin aliento: Histopatología de las enfermedades respiratorias transmisibles de los pequeños rumiantes(2013) Aguilar Moreno, Marta; Carrillo de Albornoz, Jorge; García Palencia, María Del Pilar; Martín Espada, María Del Carmen; Cid Vázquez, María DoloresElaboración de material docente