Person: Moreno García, Nerea
Universidad Complutense de Madrid
Faculty / Institute
Now showing 1 - 10 of 19
PublicationAgustín González, an inspirational leader in spanish comparative neuroanatomy(Karger, 2021-10-13) Marín, Oscar; Moreno García, NereaIt is our pleasure to open this Festschrift dedicated to our mentor Prof. Agustín González (Fig. 1) on his retirement after more than 40 years of academic work at the Universidad Complutense de Madrid. Agustín is a key figure in the field of comparative neuroanatomy, essential to understanding the evolution of this field in Spain and beyond over the last decades. This special issue collects a rich number of contributions by collaborators, pupils, and colleagues from all over the world, reflecting the impact of their achievements and the respect and admiration of the entire international community. The volume also serves as a homage to Prof. Luis Puelles, an occasional collaborator of Agustín and another giant of Spanish neuroscience. In this commentary, we summarize Agustín’s work as a researcher, teacher, mentor, and member of the scientific community. We also consider the broader impact of Agustín’s activity, primarily when it comes to its ability to influence others. As alumni from his laboratory, we do this from a very personal point of view, reflecting on our imperfect memories of the time we spent together. We wish that this imperfect historical glimpse on Agustín’s professional career will bring a smile to the face of the many colleagues who have worked with Agustín over the past few decades and inspire those who have not personally known him. PublicationDigitalización de la histoteca de las prácticas de Organografía Microscópica(2018-05-31) Morona Arribas, Ruth; González Gallegos, Agustín; Gutiérrez Cañas, Irene; García-Ceca Hernández, José Javier; López Redondo, Jesús María; Alfaro Sánchez, David; Muñiz Hernando, Enriqueta; Carrión Caballo, Mar; Moreno García, Nerea; Pérez García, Selene; Pérez Gomáriz, Rosa MaríaHistoteca digital para que los alumnos puedan consultar un material gráfico actualizado y concreto, similar a lo que observan en el microscopio cuando realizan sus prácticas de Organografía de la asignatura de “Organografía microscópica” del Grado en Biología. PublicationThe Application of 3D Anatomy for Teaching Veterinary Clinical Neurology(MDPI, 2023-05-10) Blázquez Llorca, Lidia; Morales de Paz, Lubna; Martín Ortí, Rosario; Santos Álvarez, Inmaculada; Fernández Valle, María E.; Castejón, David; García Real, María Isabel; Salguero Fernández, Raquel; Pérez Lloret, Pilar; Moreno García, Nerea; Jímenez, Sara; Herrero Fernández, María Josefa; González Soriano, Juncal MilagrosNeuroanatomy is always a challenging topic for veterinary students. It is widely accepted that understanding the anatomy of the central nervous system (CNS) is essential to explain many of the pathological processes that affect the brain. Although its study has varied over time to achieve this goal, in human and veterinary medicine it is difficult to find a teaching method that associates normal anatomy with pathological alterations of the brain. For the first time, we have created an educational tool that combines neuroanatomy and neuropathology, using different magnetic resonance (MR) images as a basis and EspINA software as analyzer, to obtain segmented structures and 3D reconstructions of the dog brain. We demonstrate that this combination is an optimal tool to help anatomists to understand the encephalon, and additionally to help clinicians to recognize illness including a multitude of neurological problems. In addition, we have tried to see whether photogrammetry, which is a common technique in other sciences, for example geology, could be useful to teach veterinary neuroanatomy. Although we still need further investigations, we have been able to generate 3D reconstructions of the whole brain, with very promising results to date. PublicationPax6 expression highlights regional organization in the adult brain of lungfishes, the closest living relatives of land vertebrates(Wiley, 2019-07-12) López Redondo, Jesús María; Morona Arribas, Ruth; Moreno García, Nerea; Lozano Rebollo, Daniel; Jiménez Álvarez, Sara; González Gallegos, AgustínThe Pax6 gene encodes a regulatory transcription factor that is key in brain develop-ment. The molecular structure of Pax6, the roles it plays and its patterns of expres-sion in the brain have been highly conserved during vertebrate evolution. Asneurodevelopment proceeds, the Pax6 expression changes from the mitotic germinalzone in the ventricular zone to become distributed in cell groups in the adult brain.Studies in various vertebrates, from fish to mammals, found that the Pax6 expressionis maintained in adults in most regions that express it during development. Specifi-cally, in amphibians, Pax6 is widely expressed in the adult brain and its distributionpattern serves to highlight regional organization of the brain. In the present study, weanalyzed the detailed distribution of Pax6 cells in the adult central nervous systemof lungfishes, the closest living relatives of all tetrapods. Immunohistochemistryperformed using double labeling techniques with several neuronal markers of knowndistribution patterns served to evaluate the actual location of Pax6 cells. Our resultsshow that the Pax6 expression is maintained in the adult brain of lungfishes, in dis-tinct regions of the telencephalon (pallium and subpallium), diencephalon, mesen-cephalon, hindbrain, spinal cord, and retina. The pattern of Pax6 expression is largelyshared with amphibians and helps to understand the primitive condition that wouldhave characterized the common ancestors to all sarcopterygians (lobe-finned fishes and tetrapods), in which Pax6 would be needed to maintain specific entities of sub-populations of neurons. PublicationSex determination in two species of anuran amphibians by magnetic resonance imaging and ultrasound techniques(MDPI, 2020-11-20) Ruiz Fernández, María José; Jiménez, Sara; Fernández Valle, Encarnación; García Real, María Isabel; Castejón Ferrer, David; Moreno García, Nerea; Ardiaca García, María; Montesinos, Andrés; Ariza, Salvador; González Soriano, JuncalThe objective of the present study was to evaluate whether gender determination in two amphibian species (Kaloula pulchra and Xenopus laevis) can be reliably carried out by means of magnetic resonance imaging (benchtop magnetic resonance imaging; BT-MRI) or ultrasound (high-resolution ultrasound; HR-US) techniques. Two species of healthy, sexually mature anurans have been used in the present study. Eight Kaloula (blind study) and six Xenopus were used as controls. Magnetic resonance imaging experiments were carried out on a low-field (1 Tesla) benchtop-MRI (BT-MRI) system. HR-US examination was performed with high-resolution equipment. Low-field BT-MRI images provided a clear and quantifiable identification of all the sexual organs present in both genders and species. The HR-US also allowed the identification of testes and ovaries in both species. Results indicate that BT-MRI allowed a very precise sex identification in both anuran species, although its use is limited by the cost of the equipment and the need for anesthesia. HR-US allowed an accurate identification of ovaries of both species whereas a precise identification of testes is limited by the ultrasonographer experience. The main advantages of this technique are the possibility of performing it without anesthesia and the higher availability of equipment in veterinary and zoo institutions. PublicationExpression of SATB1 and SATB2 in the brain of bony fishes: what fish reveal about evolution(Springer, 2023-04-01) Lozano Reboll0, Daniel; López Redondo, Jesús María; Jiménez Álvarez, Sara; Morona Arribas, Ruth; Ruiz López, Víctor; Martínez, Ana; Moreno García, NereaSatb1 and Satb2 belong to a family of homeodomain proteins with highly conserved functional and regulatory mechanisms and posttranslational modifications in evolution. However, although their distribution in the mouse brain has been analyzed, few data exist in other non-mammalian vertebrates. In the present study, we have analyzed in detail the sequence of SATB1 and SATB2 proteins and the immunolocalization of both, in combination with additional neuronal markers of highly conserved populations, in the brain of adult specimens of different bony fish models at key evolutionary points of vertebrate diversification, in particular including representative species of sarcopterygian and actinopterygian fishes. We observed a striking absence of both proteins in the pallial region of actinopterygians, only detected in lungfish, the only sarcopterygian fish. In the subpallium, including the amygdaloid complex, or comparable structures, we identified that the detected expressions of SATB1 and SATB2 have similar topologies in the studied models. In the caudal telencephalon, all models showed significant expression of SATB1 and SATB2 in the preoptic area, including the acroterminal domain of this region, where the cells were also dopaminergic. In the alar hypothalamus, all models showed SATB2 but not SATB1 in the subparaventricular area, whereas in the basal hypothalamus the cladistian species and the lungfish presented a SATB1 immunoreactive population in the tuberal hypothalamus, also labeled with SATB2 in the latter and colocalizing with the gen Orthopedia. In the diencephalon, all models, except the teleost fish, showed SATB1 in the prethalamus, thalamus and pretectum, whereas only lungfish showed also SATB2 in prethalamus and thalamus. At the midbrain level of actinopterygian fish, the optic tectum, the torus semicircularis and the tegmentum harbored populations of SATB1 cells, whereas lungfish housed SATB2 only in the torus and tegmentum. Similarly, the SATB1 expression in the rhombencephalic central gray and reticular formation was a common feature. The presence of SATB1 in the solitary tract nucleus is a peculiar feature only observed in non-teleost actinopterygian fishes. At these levels, none of the detected populations were catecholaminergic or serotonergic. In conclusion, the protein sequence analysis revealed a high degree of conservation of both proteins, especially in the functional domains, whereas the neuroanatomical pattern of SATB1 and SATB2 revealed significant differences between sarcopterygians and actinopterygians, and these divergences may be related to the different functional involvement of both in the acquisition of various neural phenotypes. PublicationDigitalización de la histoteca de las prácticas de Biología Celular e Histología(2017-06-29) Gutiérrez Cañas, Irene; Moreno García, Nerea; Morona Arribas, Ruth; López Redondo, Jesús María; González Gallegos, Agustín PublicationConserved localization of Pax6 and Pax7 transcripts in the brain of representatives of sarcopterygian vertebrates during development supports homologous brain regionalization(Frontiers Media, 2014-08-06) Moreno García, Nerea; Joven Araus, Alberto; Morona Arribas, Ruth; Bandín Carazo, Sandra; López Redondo, Jesús María; González Gallegos, AgustínMany of the genes involved in brain patterning during development are highly conserved in vertebrates and similarities in their expression patterns help to recognize homologous cell types or brain regions. Among these genes, Pax6 and Pax7 are expressed in regionally restricted patterns in the brain and are essential for its development. In the present immunohistochemical study we analyzed the distribution of Pax6 and Pax7 cells in the brain of six representative species of tetrapods and lungfishes, the closest living relatives of tetrapods, at several developmental stages.The distribution patterns of these transcription factors were largely comparable across species. In all species only Pax6 was expressed in the telencephalon, including the olfactory bulbs, septum, striatum, and amygdaloid complex. In the diencephalon, Pax6 and Pax7 were distinct in the alar and basal parts, mainly in prosomeres 1 and 3. Pax7 specifically labeled cells in the optic tectum (superior colliculus) and Pax6, but not Pax7, cells were found in the tegmentum. Pax6 was found in most granule cells of the cerebellum and Pax7 labeling was detected in cells of the ventricular zone of the rostral alar plate and in migrated cells in the basal plate, including the griseum centrale and the interpeduncular nucleus. Caudally, Pax6 cells formed a column, whereas the ventricular zone of the alar plate expressed Pax7. Since the observed Pax6 and Pax7 expression patterns are largely conserved they can be used to identify subdivisions in the brain across vertebrates that are not clearly discernible with classical techniques. PublicationDistribution of the transcription factor islet-1 in the central nervous system of nonteleost actinopterygian fish: Relationshipwith cholinergic and catecholaminergic systems(Wiley, 2023-04-18) Lozano Rebollo, Daniel; Moreno García, Nerea; Jiménez Álvarez, Sara; Chinarro Sánchez, Adrián; Morona Arribas, Ruth; López Redondo, Jesús MaríaIslet-1 (Isl1) is one of the most conserved transcription factors in the evolution of vertebrates, due to its continuing involvement in such important functions as the differentiation of motoneurons, among other essential roles in cell fate in the forebrain. Although its functions are thought to be similar in all vertebrates, the knowledge about the conservation of its expression pattern in the central nervous system goes as far as teleosts, leaving the basal groups of actinopterygian fishes overlooked, despite their important phylogenetic position. In order to assess the extent of its conservation among vertebrates, we studied its expression pattern in the central nervous system of selected nonteleost actinopterygian fishes. By means of immunohistochemical techniques, we analyzed the Isl1 expression in the brain, spinal cord, and sensory ganglia of the cranial nerves of young adult specimens of the cladistian species Polypterus senegalus and Erpetoichthys calabaricus, the chondrostean Acipenser ruthenus, and the holostean Lepisosteus oculatus. We also detected the presence of the transcription factor Orthopedia and the enzymes tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT) to better locate all the immunoreactive structures in the different brain areas and to reveal the possible coexpression with Isl1. Numerous conserved features in the expression pattern of Isl1 were observed in these groups of fishes, such as populations of cells in the subpallial nuclei, preoptic area, subparaventricular and tuberal hypothalamic regions, prethalamus, epiphysis, cranial motor nuclei and sensory ganglia of the cranial nerves, and the ventral horn of the spinal cord. Double labeling of TH and Isl1 was observed in cells of the preoptic area, the subparaventricular and tuberal hypothalamic regions, and the prethalamus, while virtually all motoneurons in the hindbrain and the spinal cord coexpressed ChAT and Isl1. Altogether, these results show the high degree of conservation of the expression pattern of the transcription factor Isl1, not only among fish, but in the subsequent evolution of vertebrates. PublicationDigitalización de la histoteca para las prácticas de Neuroanatomía Microscópica(2019-05-28) López Redondo, Jesús Mª; Morona Arribas, Ruth; Moreno García, Nerea; González Gallegos, AgustínEl objetivo de este proyecto es la elaboración de un banco digital de imágenes útil como material docente para los profesores, y como material didáctico para los alumnos de la asignatura de Neuroanatomía y Neurofisiología del Máster en Neurociencia.