Person:
Callejas Hervás, Carmen

First Name

Carmen

Last Name

Callejas Hervás

URI

https://hdl.handle.net/20.500.14352/74364

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Ciencias Biológicas

Department

Genética, Fisiología y Microbiología

Area

Genética

Identifiers

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Now showing 1 - 10 of 27

Genetic structure of Spanish white-clawed crayfish (Austropotamobius pallipes) populations as determined by RAPD analysis: reasons for optimism
(Aquatic conservation: marine and freshwater ecosystems, 2008) Beroiz Remírez, Beatriz; Callejas Hervás, Carmen; Alonso, Fernando; Ochando González, María Dolores
1. Spanish populations of the white-clawed crayfish have declined sharply over the last three decades. Although Austropotamobius pallipes was once widely distributed and very abundant in most of the limestone basins of the country, outbreaks of crayfish plague since 1978 have reduced its populations, and now only some 500–600 small populations are left. 2. Consequently, the species now enjoys protection under national legislation. Management decisions regarding the conservation of a threatened species require an understanding of the genetic structure of its populations. 3. Using random amplified polymorphic DNA (RAPD) fingerprinting the genetic variability of 11 populations of A. pallipes was assessed over the species' range in Spain, and their phylogenetic relationships determined. 4. Substantial genetic differentiation was detected among the populations tested; no clear relationship was found between patterns of genetic variability and hydrological basin. The RAPD markers showed the degree of genetic variability of these populations to be similar to, and in some cases slightly higher than, that reported in previous studies on other Spanish and European populations of A. pallipes. 5. The results offer hope for the recovery of this species in Spain, and provide information that might be useful in the management of crayfish reintroduction programmes.
Project number: 74/2014
Desarrollo de un glosario de términos biológicos en lengua de signos para alumnos de educación secundaria y universitaria.
(2015) Marquina Díaz, Domingo; Santos de la Sen, Antonio; Alonso Conde, Rafael Alejandro; Portillo Corcho, Alberto; Caballero Gómez, Ana; Arahuetes Portero, Rosa María; Pérez Gomáriz, Rosa; Carballo Cuervo, Serafín; Calvo de Pablo, Pilar; Garcia Moreno, Ana; Arriero Higueras, Elena; Gómez Miguel, Begoña; Pérez Urria Carril, Elena; Gómez Flechoso, María De Los Ángeles; Callejas Hervás, Carmen; Belda Aguilar, Ignacio
Este proyecto ha permitido crear un glosario de términos biológicos en lengua de signos española. Este glosario permitirá a los alumnos sordos de enseñanza media y superior tener una mayor accesibilidad a los términos específicos de las distintas disciplinas que constituyen las Ciencias Biológicas.
New information about genetic structure of B. oleae species revealed by ISSR markers
(Proceedings of the IOBC/WPRS Working Group "Integrated Protection of Olive Crops", 2012) Hernández, Sofía; Callejas Hervás, Carmen; Matallanas, Beatriz; Ochando González, María Dolores; D. Perdikis, P. Weintraub, A. Lucchi
The olive fruit fly, Bactrocera oleae, is a major pest of olive crops and its expansion isrestricted to the geographic areas where olive trees are grown. The knowledge of the within andbetween populations genetic variability can help to understand the history of a species andmonitoring the origin and spread of invading populations. Such information could be crucial todefine appropriate strategies for eradication or control.In the present work, PCR amplification ofinter-simple sequence repeats (ISSR technique) was applied to the analysis of the geneticvariability of four Iberian populations of B. oleae. Flies from four different geographical areas,representing the Iberian distribution range of the species, were collected by harvesting infestedfruit and allowing the larvae to pupate in the laboratory. Four random primers (817, 820, 820,847, UBC primer set no. 9) were used to assess their genetic variation. The results showconsiderable levels of genetic polymorphism in the analysed samples, ranging from 78.5% to82.1%. Regarding the distribution of this variability, most of the genetic variation was foundwithin populations (92.6%). Likewise, a substantial level of gene flow (5.36) was deduced. Thus,ISSR are highly sensitive markers for variability detection in B. oleae and could help to answerfundamental questions related to the population structure and dynamics of the fly and, hence, toimprove management control.
Project number: 238
Zoología. Un modelo para la enseñanza no presencial
(2021) García Moreno, Ana María; Callejas Hervás, Carmen; González Jaén, María Teresa; Martín Ortí, Rosario; Muñoz Araujo, Benito; Outerelo Domínguez, Raimundo; Pardos Martínez, Fernando; Refoyo Román, Pablo Alberto; de La Cruz Orobio, Raúl; González Cánovas, Rosario V.; Ruiz Piña, Eduardo; Cepeda Gómez, Diego; Castelló Fortet, José Ramón; Roca Juncosa, Joan
Memoria Proyecto de innovación nº 238 curso 2020/21
Specific and sensitive primers for the detection of predated olive fruit flies, Bactrocera oleae (Diptera: Tephritidae)
(Spanish Journal of Agricultural Research, 2017) Lantero, Esther; Matallanas Peñas, Beatriz; Ochando González, María Dolores; Pascual, Susana; Callejas Hervás, Carmen
Bactrocera oleae, the olive fruit fly, is a major pest of olive (Olea europaea L.) trees worldwide. Its presence can cause important losses, with consequences for the economies of countries that produce and export table olives and olive oil. Efforts to control olive fruit fly populations have, however, been insufficient. Now more than ever, environmentally friendly alternatives need to be considered in potential control programs. Generalist predators could provide a way of managing this pest naturally. However, the identification of candidate predator species is essential if such a management system is to be introduced. The present paper describes a set of species-specific primers for detecting the presence of B. oleae DNA in the gut of predatory arthropods. All primers were tested for checking cross-reactive amplification of other fruit fly DNA and evaluated in heterospecific mixes of nucleic acids. All were found to be very sensitive for B. oleae. Subsequent feeding trials were conducted using one of the most abundant species of ground dwelling carabids in olive groves in south-eastern Madrid, Spain. These trials allowed determining that 253F-334R and 334F-253R primer pairs had the highest detection efficiency with an ID50 of around 78 h. These primers therefore provide a very useful tool for screening the gut contents of potential predators of B. oleae, and can thus reveal candidate species for the pest's biological control.
Mitochondrial DNA variability in Spanish populations of A. italicus inferred from the analysis of a COI region
(Knowledge and Management of Aquatic Ecosystems, 2011) Matallanas Peñas, Beatriz; Ochando González, María Dolores; Vivero, Almudena; Beroiz Remírez, Beatriz; Alonso, Fernando; Callejas Hervás, Carmen
Austropotamobius italicus was once widely distributed throughout most of the country’s limestone basins in Spain. But its populations have shown a very strong decline over the last thirty years, due to different factors. Thus, the species now enjoys protection under regional, national and international legislation. Therefore, knowledge of the levels and patterns of distribution of genetic diversity in crayfish populations is critical when making conservation management decisions. In the present work, the current genetic structure of Spanish populations of white-clawed crayfish, A. italicus, was analyzed. Eleven Spanish populations and an Italian sample were studied through an 1184 bp-lentgh sequence of cytochrome oxidase subunit I mitochondrial gene. Data analysis revealed the existence of eight haplotypes in the Iberian Peninsula, the highest diversity reported to date in Spanish crayfish. Also a substantial genetic differentiation among populations was found, with a clear geographic pattern. The genetic variability found in these populations is similar to, and even higher, than that reported in previous studies on other Spanish and European populations of A. italicus. Thus, given the current risk status of the species across its range, this variability in certain populations offers some hope for the species from a management point of view.
Project number: 106
Diseño y utilización de unas prácticas de Regulación del Metabolismo como herramienta integradora de conocimientos multidisciplinares en el Grado en Biología (II)
(2017) Guillén Maestro, Alberto; Pérez Uz, María Blanca; Serrano Barrero, Susana Lourdes; Arregui García-Rovés, Lucía; Callejas Hervás, Carmen; Linacero de la Fuente, Rosario; Acebal Sarabia, Carmen; Megías Fresno, Alicia; Gómez Miguel, Begoña
Se pretende la creación de espacios multidisdiplinares en el Grado en Biología,con prácticas integradas de asignaturas que se cursan simultáneamente, que pudieran servir de base a propuestas de Trabajos de Fin de Grado.
Genetic structure of Spanish populations of Ceratitis capitata revealed by RAPD and ISSR markers: implications for resistance management
(Spanish Journal of Agricultural Research, 2012) Beroiz Remírez, Beatriz; Ortego, Félix; Callejas Hervás, Carmen; Hernández-Crespo, Pedro; Castañera, Pedro; Ochando González, María Dolores
The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is considered one of the most economically damaging pests of citrus orchards in Spain. The characterization of C. capitata population structure, at a large geographical scale, by using a combination of RAPD and ISSR markers can allow analyzing the genetic variability of this species, and provide some insight in decision making for resistance management, recently recorded in Spain. We compared six Spanish populations along the Mediterranean area (Gerona, Amposta, Tortosa, Castellón, Valencia and Málaga) with populations from other geographical areas where this pest is widely distributed (Africa, Middle East, South America and Atlantic Islands) and two laboratory strains. The results obtained with both types of molecular markers were similar. A dendrogram based on Nei genetic distances showed that all Mediterranean Spanish populations, except the population collected in Gerona, were clearly separated from the rest. However, no clear differentiation among Spanish populations was found, probably as a result of the high levels of gene flow (Nm value of 2.8 for RAPD and 3.9 for ISSR). Implications of these findings on resistance management of C. capitata are discussed.
Genetic polymorphism at the cytochrome oxidase I gene in Mediterranean populations of Batrocera oleae (Diptera: Tephritidae)
(Journal of Applied Entomology, 2013) Matallanas Peñas, Beatriz; Lantero, Esther; M'Saad, Meriem; Callejas Hervás, Carmen; Ochando González, María Dolores
The tephritid Bactrocera oleae (Gmelin) is a harmful pest of olive crops that cause important agricultural and economic losses in the Mediterranean area where 90% of the world olive trees are cultivated. The knowledge of the genetic diversity in insect pest species populations is critical for decisions concerning appropriate control management strategies. In the presnt work, the genetic variability within and among 7 populations -five from Spain, one from Italy and one from Tunisia-, was assessed by sequencing 1151 bp of the COI gene. A total of 21 haplotypes were observed. The intraspecific diversity was high, particularly in the Spanish populations (haplotype and nucleotide diversity 0.84 and 0.00137, respec tively). However, the genetic differentiation among the populations was low in the case of Spanish ones (Fst between 0 and 0.041), and higher – and statistically significant– when comparing with the Italian and Tuni sian samples. The haplotypes distribution and the PCoA analysis show three clear groups of populations: Spanish, Italian and Tunisian. The results might indicate the length of time elapsed since B. oleae became established in the Mediterranean region, the large effective sizes expected of its populations and the high gene flow among Iberian populations. The information could be relevant for integrated control programmes coordination.
Current Status of the Main Olive Pests: Useful Integrated Pest Management Strategies and Genetic Tools
(Applied Sciences, 2023) Lantero, Esther ; Matallanas Peñas, Beatriz; Callejas Hervás, Carmen
Mediterranean olive cultivation faces challenges in the global environmental change context. Pests and diseases caused by arthropods such as Bactrocera oleae, Prays oleae, and certain vectors of Xylella fastidiosa are expected to increase and spread in part due to this global scenario. The control of these arthropods has relied on synthetic pesticides, the misuse of which has led to pest population resistance and concerns about their negative impacts on biodiversity and global health. Integrated pest management (IPM) methods have emerged through the careful consideration of all available control techniques and the subsequent integration of appropriate measures that discourage the development of pest populations. This paper reviews the IPM guidelines for olive cultivation, prioritizing the use of biological control methods, and the integration of genetics and biotechnology, which bring precision, efficacy, and safety. It evidences the importance of genetic analysis in pest populations, pesticide resistance and in the contributions of predators to pest control. Advances in formulations and delivery systems for pesticides such as Bacillus thuringiensis, plant-incorporated protectants, improved SIT techniques, and the specific efficacy of biologicals pesticides are covered. Finally, this paper explores promising tools such as RNAi and gene drive while recognizing the ethical, environmental, and regulatory challenges associated with their use. Shortly, these innovations have the potential to reduce the environmental impacts of pests while ensuring the long-term viability of the olive industry.