Person:
Pedrero Muñoz, María

First Name

María

Last Name

Pedrero Muñoz

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Ciencias Químicas

Department

Química Analítica

Area

Química Analítica

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Now showing 1 - 10 of 21

Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials
(Wiley, 2022-04-28) Campuzano Ruiz, Susana; Pedrero Muñoz, María; Barderas, Rodrigo; Pingarrón Carrazón, José Manuel
Electrochemical biosensors continue to evolve at an astonishing pace, consolidating as competitive tools for determining a wide range of targets and relentlessly strengthening their attributes in terms of sensitivity, selectivity, simplicity, response time, and antifouling ability, making them suitable for getting a foothold in real-world applications. The design and exploitation of nanostructured or multifunctional nucleic acid or peptide biomaterials is playing a determinant role in these achievements. With the aim of highlighting the potential and opportunities of these biomaterials, this perspective article critically discusses and overviews the electrochemical biosensors reported since 2019 involving nanostructured and multifunctional DNA biomaterials, multifunctional aptamers, modern peptides, and CRISPR/Cas systems. The use of these biomaterials as recognition elements, electrode modifiers (acting as linkers or creating scaffolds with antifouling properties), enzyme substrates, and labeling/carrier agents for signal amplification is discussed through rationally and strategically selected examples, concluding with a personal perspective about the challenges to be faced and future lines of action.
Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers
(MDPI, 2016-11-21) Pedrero Muñoz, María; Manuel de Villena Rueda, Francisco Javier; Muñoz San Martín, Cristina; Campuzano Ruiz, Susana; Garranzo Asensio, María; Barderas Manchado, Rodrigo; Pingarrón Carrazón, José Manuel
An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. an Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as a redox mediator and H2O2 as the enzyme substrate. The magnetoimmunosensing platform was successfully applied for the detection of p53 protein in different cell lysates without any matrix effect after a simple sample dilution. The results correlated accurately with those provided by a commercial ELISA kit, thus confirming the immunosensor as an attractive alternative for rapid and simple determination of this protein using portable and affordable instrumentation.
Beyond Sensitive and Selective Electrochemical Biosensors: Towards Continuous, Real-Time, Antibiofouling and Calibration-Free Devices
(MDPI, 2020-06-16) Campuzano Ruiz, Susana; Pedrero Muñoz, María; Gamella Carballo, Maria; Serafín González-Carrato, Verónica; Yáñez Sedeño, Paloma; Pingarrón Carrazón, José Manuel
Nowadays, electrochemical biosensors are reliable analytical tools to determine a broad range of molecular analytes because of their simplicity, affordable cost, and compatibility with multiplexed and point-of-care strategies. There is an increasing demand to improve their sensitivity and selectivity, but also to provide electrochemical biosensors with important attributes such as near real-time and continuous monitoring in complex or denaturing media, or in vivo with minimal intervention to make them even more attractive and suitable for getting into the real world. Modification of biosensors surfaces with antibiofouling reagents, smart coupling with nanomaterials, and the advances experienced by folded-based biosensors have endowed bioelectroanalytical platforms with one or more of such attributes. With this background in mind, this review aims to give an updated and general overview of these technologies as well as to discuss the remarkable achievements arising from the development of electrochemical biosensors free of reagents, washing, or calibration steps, and/or with antifouling properties and the ability to perform continuous, real-time, and even in vivo operation in nearly autonomous way. The challenges to be faced and the next features that these devices may offer to continue impacting in fields closely related with essential aspects of people’s safety and health are also commented upon.
Affinity-Based Wearable Electrochemical Biosensors: Natural versus Biomimetic Receptors
(Wiley, 2022-11-02) Campuzano Ruiz, Susana; Pedrero Muñoz, María; Torrente Rodríguez, Rebeca Magnolia; Pingarrón Carrazón, José Manuel
This review delves into the titanic research efforts carried out during the last years on affinity-based wearable electrochemical biosensors, using both natural (antibodies) and biomimetic (aptamers, peptides and molecular imprinted polymers) receptors. The rationale and application of selected representative strategies is critically discussed, ending with realistic and futuristic visions of the technical barriers, challenges and prospects in the development and adoption of these biodevices in daily routines to ensure well-being against known, unknown and unexpected threats.
Diseño y preparación de un laboratorio virtual de Química Analítica: Técnicas instrumentales de análisis
(2022-01-30) Benito Peña, Elena; Burgueño Arjona, Maria José; Campuzano Ruiz, Susana; Madrid Albarrán, María Yolanda; Marazuela Lamata, María Dolores; Mateos Briz, María Raquel; Moreno Bondi, María Cruz; Navarro Villoslada, Fernando; Pedrero Muñoz, María; Reviejo García, Ángel Julio; Tortajada Pérez, José; Urraca Ruiz, Javier; Villalonga Santana, Reynaldo; Blanco Asenjo, Miriam; del Valle Ávila, Marcos; Molla Escudero, David; Navarro Duro, Marina; del Rosario García-Marcos, Beatriz; Merino Sierra, Miguel Ángel; García López, Patricia
La reciente pandemia del COVID-19 ha supuesto un cambio excepcional y drástico de la concepción tradicional del aprendizaje, tanto para los estudiantes como para los docentes. Ante esta situación se requiere no sólo acciones que faciliten la adaptación de los estudiantes y profesores a las plataformas educativas en línea, sino también a que éstas se conviertan en auténticas herramientas para potenciar y mejorar de forma significativa el aprendizaje del alumno. El presente proyecto de Innovación Educativa y Mejora de la Calidad Docente pretende mejorar la calidad del aprendizaje de varias asignaturas de los Grados de Química e Ingeniería Química que llevan asociado un Laboratorio de Técnicas Instrumentales. Tradicionalmente esa docencia práctica ha sido presencial, pero la situación excepcional surgida con la pandemia en el curso 2019/2020, ha demostrado que disponer de unas prácticas virtualizadas es de gran ayuda para facilitar el aprendizaje de los alumnos y facilitar una transferencia del conocimiento constructivo y colaborativo. Desde hace años, el personal docente e investigador (PDI), personal de administración y servicios (PAS) y los estudiantes de la UCM, disponemos de la plataforma de enseñanza online Moodle (Campus Virtual, CV). Sin embargo, la pandemia que vivimos ha evidenciado la brecha digital en lo referente a cómo usar Moodle y, por ende, la utilización de las Tecnologías de Información y Comunicación (TICs) como facilitadoras didácticas. A pesar de nuestras limitaciones, tanto profesores como estudiantes, hemos desarrollado una enorme capacidad resiliente, lo que permitió, durante el pasado mes de mayo, la puesta en marcha de los primeros laboratorios en línea en el Departamento de Química Analítica de la UCM. Fruto de esta experiencia, así como de la situación actual de incertidumbre para el curso próximo, algunos profesores, estudiantes y PAS del departamento hemos decidido adelantarnos a un escenario futuro en el que se contemple nuevamente la impartición de Docencia Experimental en línea y solicitar el presente proyecto de Innovación Educativa y Mejora de la Calidad Docente.
Implementación de la metodología flipped classroom en los laboratorios de Química Analítica
(2023-05-31) Reviejo García, Ángel Julio; Agüí Chicharro, María Lourdes; Campuzano Ruiz, Susana; Gamella Carballo, Maria; García Martín, Ángel Felipe; González Cortés, Araceli; Guerrero Blanco, José Ignacio; Mateos Briz, María Raquel; Miguel Bravo, María; Pérez Ginés, Víctor; Reviejo Martínez, Eva; Romano Martín, Santiago; Ruiz-Valdepeñas Montiel, Víctor; Sánchez Tirado, Esther; Santiago Sáez, Andrés Sebastián; Serafín González-Carrato, Verónica; Torrente Rodríguez, Rebeca Magnolia; Yáñez-Sedeño, Paloma; Pedrero Muñoz, María
Adaptar el sistema tradicional de aprendizaje a las necesidades actuales del alumnado empleando la metodología flipped classroom en el laboratorio de Química Analítica I, con el objetivo de fomentar el aprendizaje utilizando herramientas digitales.
Quantum Dots as Components of Electrochemical Sensing Platforms for the Detection of Environmental and Food Pollutants: a Review
(AOAC, 2017) Pedrero Muñoz, María; Campuzano, Susana; Pingarrón Carrazón, José Manuel
The determination of organic and inorganic environmental and food pollutants is a key matter of concern in analytical chemistry due to their effects as a serious threat to human health. Focusing on this issue, several methodologies involving the use of nanostructured electrochemical platforms have been recently reported in the literature. Among these methods, those employing the use of quantum dots (QDs) stand out because of features such as signal amplification, good reproducibility and selectivity, and the possibility for multiplexed detection, and because they preserve the outstanding characteristics of electrochemical methodologies with respect to simplicity, ease-of-use, and cost-effective instrumentation. This review describes recent electrochemical strategies, in which design QDs play a key role, for the determination of pollutants in food and environmental samples. The particular role of QDs in the reported methodologies, their preparation, and the electrochemical platform design, as well as the advantages that QDs provide in the analysis of target analytes, are critically discussed.
Biosensing and Delivery of Nucleic Acids Involving Selected Well-Known and Rising Star Functional Nanomaterials
(MDPI, 2019-11-14) Campuzano Ruiz, Susana; Gamella Carballo, Maria; Serafín González-Carrato, Verónica; Pedrero Muñoz, María; Yáñez Sedeño, Paloma; Pingarrón Carrazón, José Manuel
In the last fifteen years, the nucleic acid biosensors and delivery area has seen a breakthrough due to the interrelation between the recognition of nucleic acid’s high specificity, the great sensitivity of electrochemical and optical transduction and the unprecedented opportunities imparted by nanotechnology. Advances in this area have demonstrated that the assembly of nanoscaled materials allows the performance enhancement, particularly in terms of sensitivity and response time, of functional nucleic acids’ biosensing and delivery to a level suitable for the construction of point-of-care diagnostic tools. Consequently, this has propelled detection methods using nanomaterials to the vanguard of the biosensing and delivery research fields. This review overviews the striking advancement in functional nanomaterials’ assisted biosensing and delivery of nucleic acids. We highlight the advantages demonstrated by selected well-known and rising star functional nanomaterials (metallic, magnetic and Janus nanomaterials) focusing on the literature produced in the past five years.
Magnetic Beads-Based Sensor with Tailored Sensitivity for Rapid and Single-Step Amperometric Determination of miRNAs
(MDPI, 2017-11-09) Vargas Orgaz, Eva; Torrente Rodríguez, Rebeca Magnolia; Ruiz Valdepeñas Montiel, Víctor; Povedano Muñumel, Eloy; Pedrero Muñoz, María; Montoya, Juan; Campuzano Ruiz, Susana; Pingarrón Carrazón, José Manuel
This work describes a sensitive amperometric magneto-biosensor for single-step and rapid determination of microRNAs (miRNAs). The developed strategy involves the use of direct hybridization of the target miRNA (miRNA-21) with a specific biotinylated DNA probe immobilized on streptavidin-modified magnetic beads (MBs), and labeling of the resulting heteroduplexes with a specific DNA–RNA antibody and the bacterial protein A (ProtA) conjugated with an horseradish peroxidase (HRP) homopolymer (Poly-HRP40) as an enzymatic label for signal amplification. Amperometric detection is performed upon magnetic capture of the modified MBs onto the working electrode surface of disposable screen-printed carbon electrodes (SPCEs) using the H2O2/hydroquinone (HQ) system. The magnitude of the cathodic signal obtained at −0.20 V (vs. the Ag pseudo-reference electrode) demonstrated linear dependence with the concentration of the synthetic target miRNA over the 1.0 to 100 pM range. The method provided a detection limit (LOD) of 10 attomoles (in a 25 μL sample) without any target miRNA amplification in just 30 min (once the DNA capture probe-MBs were prepared). This approach shows improved sensitivity compared with that of biosensors constructed with the same anti-DNA–RNA Ab as capture instead of a detector antibody and further labeling with a Strep-HRP conjugate instead of the Poly-HRP40 homopolymer. The developed strategy involves a single step working protocol, as well as the possibility to tailor the sensitivity by enlarging the length of the DNA/miRNA heteroduplexes using additional probes and/or performing the labelling with ProtA conjugated with homopolymers prepared with different numbers of HRP molecules. The practical usefulness was demonstrated by determination of the endogenous levels of the mature target miRNA in 250 ng raw total RNA (RNAt) extracted from human mammary epithelial normal (MCF-10A) and cancer (MCF-7) cells and tumor tissues.
Determinación de herbicidas nitrofenoles y s-triazinas por voltamperometría de adsorción-redisolución
(Universidad Complutense de Madrid, Servicio de Publicaciones, 2002) Pedrero Muñoz, María; Manuel de Villena Rueda, Francisco Javier; Pingarrón Carrazón, José Manuel
En este trabajo se han desarrollado métodos para determinar los herbicidas dinoseb, metoprotrina y terbutrina empleando polarografía diferencial de impulsos (dpp) y voltamperometria de adsorción-redisolución (adsv). A partir del comportamiento polarográfico de los tres herbicidas se han establecido las caracteristicas del proceso electrodo proponiéndose los mecanismos de reducción correspondientes y métodos para su determinación mediante dpp que se han aplicado para determinar dinoseb en manzanas y metoprotrina y terbutrina en peras. Tras verificar la posibilidad de utilizar la adsorción como etapa de preconcentración efectiva, se han desarrollado métodos adsv para determinar los tres herbicidas sobre el electrodo de gota colgante de mercurio (hmde). Se han establecido sus caracteristicas analíticas y se han aplicado a la determinación de dinoseb en manzanas y zumo de manzana y de metoprotrina y terbutrina individualmente en aguas potables y procedentes de riego. Asimismo, se ha desarrollado un método para determinar dinoseb por adsv sobre el electrodo de película de mercurio, mejorando el límite de determinación del orden de 10 veces con respecto al obtenido sobre el hmde. Este método se ha aplicado a la determinación del herbicida en muestras de zumo de manzana. Finalmente, se ha evaluado la posibilidad de aplicar la técnica adsv en medios dispersos. Determinando terbutrina en medio micelar formado con pentanosulfonato sódico y en medio emulsionado constituido con acetato de etilo se han obtenido limites de determinación del mismo orden que en disoluciones acuosas.