Person: Colilla Nieto, Montserrat
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First Name
Montserrat
Last Name
Colilla Nieto
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
Química Inorgánica
Identifiers
33 results
Search Results
Now showing 1 - 10 of 33
Item Preventing bacterial adhesion on scaffolds for bone tissue engineering(International Journal of Bioprinting, 2016) Sánchez Salcedo, Sandra; Colilla Nieto, Montserrat; Izquierdo Barba, Isabel; Vallet Regí, María Dulce NombreBone implant infection constitutes a major sanitary concern which is associated to high morbidity and health costs. This manuscript focused on overviewing the main research efforts committed up to date to develop innovative alternatives to conventional treatments, such as those with antibiotics. These strategies mainly rely on chemical modifi-cations of the surface of biomaterials, such as providing it of zwitterionic nature, and tailoring the nanostructure surface of metal implants. These surface modifications have successfully allowed inhibition of bacterial adhesion, which is the first step to implant infection, and preventing long-term biofilm formation compared to pristine materials. These strate-gies could be easily applied to provide three-dimensional (3D) scaffolds based on bioceramics and metals, of which its manufacture using rapid prototyping techniques was reviewed. This opens the gates for the design and development of advanced 3D scaffolds for bone tissue engineering to prevent bone implant infections. Keywords: Antibacterial adhesion, biofilm formation, zwitterionic surfaces, nanostructured surfaces, rapid prototyping 3D scaffolds, bone tissue engineering.Item Engineering Mesoporous Silica Nanoparticles for Drug Delivery: where are we after two decades?(Chemical Society Reviews, 2022) Vallet Regí, María Dulce Nombre; Schüth, Ferdi; Lozano Borregón, Daniel; Colilla Nieto, Montserrat; Manzano García, MiguelThe present review details a chronological description of the events that took place during the development of mesoporous materials, their different synthetic routes and their use as drug delivery systems. The outstanding textural properties of these materials quickly inspired their translation to the nanoscale dimension leading to mesoporous silica nanoparticles (MSNs). The different aspects of introducing pharmaceutical agents into the pores of these nanocarriers, together with their possible biodistribution and clearance routes, would be here described. The development of smart nanocarriers able to release high local concentration of the therapeutic cargo on-demand after the application of certain stimuli would be here reviewed, together with the ability of delivering the therapeutic cargo to precise locations in the body. The huge progress in the design and development of MSNs for biomedical applications, including the potential treatment of different diseases, during the last 20 years will be here collected, together with the required work that still needs to be done to achieve the clinical translation of these materials. This review was conceived to stand out from past reports since aims to tell the story of the development of mesoporous materials and their use as drug delivery systems by some of the story makers, who could be considered to be among the pioneers in this area.- Project number: 52
Item Aprendizaje autónomo del laboratorio de Química Bioinorgánica y Biomateriales y técnicas de caracterización utilizadas a través del uso de herramientas e-learning. Potenciación de la comprensión de alumnos con diversidad, dificultades auditivas o idioma(2022) Sanchez Salcedo, Sandra; Pablos, Jesús Luis; Peña Lopez, Juan; Arcos Navarrete, Daniel; Doadrio Villarejo, Antonio Luis; Garcia Fontecha, Ana; Colilla Nieto, Montserrat; Gonzalez Ortiz, Blanca; Cabañas Criado, María Victoria; Cicuéndez Maroto, Mónica; Salinas Sanchez, Antonio Jesús; Lozano Borregon, Daniel; Manzano Garcia, Miguel; Izquierdo Barba, Isabel; Jimenez Holguin, Javier; Estevez Amado, Manuel; Doadrio Villarejo, Juan Carlos; Moreno Pérez, José ManuelEl proyecto tiene como objetivo fundamental la elaboración de material audiovisual para la mejora de las prácticas de la asignatura de Química Bioinorgánica y Biomateriales de cuarto curso del grado en Farmacia para su satisfactorio desarrollo tanto en un escenario totalmente on-line como presencial. Para el buen desarrollo de las prácticas en el laboratorio es necesario que el alumno disponga de una información adecuada que le permita conocer los distintos aspectos del proceso experimental a realizar a través de la elaboración de una página web disponible en el campus virtual. Para ello, la práctica habitual es utilizar un manual de prácticas de laboratorio en el que se aporta información sobre conceptos básicos, material a utilizar, procedimiento experimental, seguridad, etc… de cada práctica. Hay tres aspectos de gran importancia desde el punto de vista formativo: 1) afianzar bien los conceptos básicos en los que se apoya el trabajo experimental posterior, 2) el manejo de los software que permitan el tratamiento de los resultados e interpretación de los datos utilizando herramientas e-learning y 3) facilitar la comprensión de los dos objetivos anteriores a personas con discapacidades auditivas y/o idioma incluyendo subtítulos en español e inglés en los videos elaborados. En dicha elaboración se tendrá muy en cuenta, la inclusión de los alumnos con diversidad presentando dicha información de manera visual, estructurada, secuencial y en pasos ordenados. Estas herramientas de aprendizaje estarán dirigidas inicialmente a los alumnos de cursos superiores del Grado en Farmacia, Química, Ingeniería de Materiales y Máster Universitario en Biomateriales por lo que para comprobar su impacto y eficacia seleccionaemos a estudiantes de los departamentos implicados antes de ponerlos a disposición de los alumnos. Este proyecto se basa en tres Competencias transversales instrumentales del Espacio Europeo de Educación Superior (EEES): Comunicación oral y escrita en la/s lengua/s materna/s, Comunicación en lengua extranjera, y Utilización de las Tecnologías de la información y la Comunicación (TIC) en el ámbito de estudio y contexto profesional. Estas competencias son clave para el desarrollo académico y profesional de los alumnos/as, especialmente para alumnos/as con algún tipo de discapacidad. Creemos que, mediante este proyecto, se realiza un esfuerzo para mejorar la inclusión de estos alumnos/as tanto en un contexto online como presencial en un laboratorio de Química. Por las razones anteriormente expuestas, un grupo de profesores de los Departamentos de Química en Ciencias Farmacéuticas y el de Bioquímica y Biología Molecular se propone elaborar un material didáctico que permita el autoaprendizaje del alumno en la parte experimental de la asignatura optativa de Química Bioinorgánica y Biomateriales del Grado en Farmacia, si bien, este material también será de utilidad para complementar otras asignaturas presentes en los Grados de Biología o de Ingeniería de Materiales e, incluso en el Máster en Biomateriales de la UCM. Item Lectin-Conjugated pH-Responsive Mesoporous Silica Nanoparticles for Targeted Bone Cancer Treatment.(Acta Biomaterialia, 2017) Martínez Carmona, Marina; Lozano Borregón, Daniel; Colilla Nieto, Montserrat; Vallet Regí, María Dulce NombreA novel multifunctional nanodevice based in doxorubicin (DOX)-loaded mesoporous silica nanoparticles (MSNs) as nanoplatforms for the assembly of different building blocks has been developed for bone cancer treatment. These building blocks consists of: i) a polyacrylic acid (PAA) capping layer grafted to MSNs via an acid-cleavable acetal linker, to minimize premature cargo release and provide the nanosystem of pH-responsive drug delivery ability; and ii) a targeting ligand, the plant lectin concanavalin A (ConA), able to selectively recognize, bind and internalize owing to certain cell-surface glycans, such as sialic acids (SA), overexpressed in given tumor cells. This multifunctional nanosystem exhibits a noticeable higher internalization degree into human osteosarcoma cells (HOS), overexpressing SA, compared to healthy preosteoblast cells (MC3T3-E1). Moreover, the results indicate that small DOX loading (2.5 µg mL−1) leads to almost 100% of osteosarcoma cell death in comparison with healthy bone cells, which significantly preserve their viability. Besides, this nanodevice has a cytotoxicity on tumor cells 8-fold higher than that caused by the free drug. These findings demonstrate that the synergistic combination of different building blocks into a unique nanoplatform increases antitumor effectiveness and decreases toxicity towards normal cells. This line of attack opens up new insights in targeted bone cancer therapy. Statement of Significance The development of highly selective and efficient tumor-targeted smart drug delivery nanodevices remains a great challenge in nanomedicine. This work reports the design and optimization of a multifunctional nanosystem based on mesoporous silica nanoparticles (MSNs) featuring selectivity towards human osteosarcoma cells and pH-responsive antitumor drug delivery capability. The novelty and originality of this manuscript relies on proving that the synergistic assembly of different building blocks into a unique nanoplatform increases antitumor effectiveness and decreases toxicity towards healthy cells, which constitutes a new paradigm in targeted bone cancer therapy.Item Recent advances in mesoporous silica nanoparticles for antitumor therapy: our contribution(Biomaterials Science, 2016) Baeza García, Alejandro; Manzano García, Miguel; Colilla Nieto, Montserrat; Vallet Regí, María Dulce NombreSince 2001, when our research group proposed for the first time MCM-41 as a drug release system, the scientific community has demonstrated a growing interest in mesoporous silica nanoparticles (MSNs) for their revolutionary potential in nanomedicine. Among the diverse pathologies that can be treated with MSNs, cancer has received increasing attention. MSNs can be loaded with large amounts of therapeutic cargoes and once intravenously administrated preferentially accumulate in solid tumours via the enhanced permeation and retention (EPR) effect. Herein we report the recent developments achieved by our research group as a pioneer in this field, highlighting: the design of sophisticated MSNs as stimuli-responsive drug delivery systems to release the entrapped cargo upon exposure to a given stimulus while preventing the premature release of highly cytotoxic drugs before reaching the target; transporting non-toxic prodrugs and the enzyme responsible for its conversion into cytotoxic agents into the same MSNs; improving the selectivity and cellular uptake by cancer cells by active targeting of MSNs; increasing the penetration of MSNs within the tumour mass, which is one of the major challenges in the use of NPs to treat solid tumours.Item Amine-Functionalized Mesoporous Silica Nanoparticles: A New Nanoantibiotic for Bone Infection Treatment(Biomedical Glasses, 2018) Pedraza, Daniel; Díez, Jaime; Izquierdo Barba, Isabel; Colilla Nieto, Montserrat; Vallet Regí, María Dulce NombreThis manuscript reports an effective new alternative for the management of bone infection by the 5 development of an antibiotic nanocarrier able to penetrate bacterial biofilm, thus enhancing antimicrobial effectiveness. This nanosystem, also denoted as “nanoantibiotic”, consists in mesoporous silica nanoparticles (MSNs) loaded with an antimicrobial agent (levofloxacin, LEVO) 10 and externally functionalized with N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (DAMO) as targeting agent. This amine functionalization provides MSNs of positive charges, which improves the affinity towards the negatively charged bacteria wall and biofilm. Physical and 15 chemical properties of the nanoantibiotic were studied using different characterization techniques, including Xray diffraction (XRD), transmission electron microscopy (TEM), N2 adsorption porosimetry, elemental chemical analysis, dynamic light scattering (DLS), zeta (� )-potential 20 and solid-state nuclear magnetic resonance (NMR). “In vial” LEVO release profiles and the in vitro antimicrobial effectiveness of the different released doses were investigated. The efficacy of the nanoantibiotic against a S. aureus biofilm was also determined, showing the practically total 25 destruction of the biofilmdue to the high penetration ability of the developed nanosystem. These findings open up promising expectations in the field of bone infection treatment.Item Concanavalin A-targeted mesoporous silica nanoparticles for infection treatment.(Acta Biomaterialia, 2019) Martínez Carmona, Marina; Izquierdo Barba, Isabel; Colilla Nieto, Montserrat; Vallet Regí, María Dulce NombreThe ability of bacteria to form biofilms hinders any conventional treatment for chronic infections and has serious socio-economic implications. For this purpose, a nanocarrier capable of overcoming the barrier of the mucopolysaccharide matrix of the biofilm and releasing its loadedantibiotic within this matrix would be desirable. Herein, we developed a new nanosystem based on levofloxacin (LEVO)-loaded mesoporous silica nanoparticles (MSNs) decorated with the lectin concanavalin A (ConA). The presence of ConA promotes the internalization of this nanosystem into the biofilm matrix, which increases the antimicrobial efficacy of the antibiotic hosted within the mesopores. This nanodevice is envisioned as a promising alternative to conventional treatments for infection by improving the antimicrobial efficacy and reducing side effects.Item Impact of the antibiotic-cargo from MSNs on gram-positive and gram-negative bacterial biofilms.(Microporous and Mesoporous Materials, 2020) Aguilar Colomer, Anna; Colilla Nieto, Montserrat; Izquierdo Barba, Isabel; Jiménez Jiménez, Carla; Mahillo, Ignacio; Esteban, Jaime; Vallet Regí, María Dulce NombreMesoporous silica nanoparticles (MSNs) are promising drug nanocarriers for infection treatment. Many investigations have focused on evaluating the capacity of MSNs to encapsulate antibiotics and release them in a controlled fashion. However, little attention has been paid to determine the antibiotic doses released from these nanosystems that are effective against biofilm during the entire release time. Herein, we report a systematic and quantitative study of the direct effect of the antibiotic-cargo released from MSNs on Gram-positive and Gram-negative bacterial biofilms. Levofloxacin (LVX), gentamicin (GM) and rifampin (RIF) were separately loaded into pure-silica and amino-modified MSNs. This accounts for the versatility of these nanosystems since they were able to load and release different antibiotic molecules of diverse chemical nature. Biological activity curves of the released antibiotic were determined for both bacterial strains, which allowed to calculate the active doses that are effective against bacterial biofilms. Furthermore, in vitro biocompatibility assays on osteoblast-like cells were carried out at different periods of times. Albeit a slight decrease in cell viability was observed at the very initial stage, due to the initial burst antibiotic release, the biocompatibility of these nanosystems is evidenced since a recovery of cell viability was achieved after 72 h of assay. Biological activity curves for GM released from MSNs exhibited sustained patterns and antibiotic doses in the 2-6 µg/mL range up to 100 h, which were not enough to eradicate biofilm. In the case of LVX and RIF first-order kinetics featuring an initial burst effect followed by a sustained release above the MIC up to 96 h were observed. Such doses reduced by 99.9% bacterial biofilm and remained active up to 72 h with no emergence of bacterial resistance. This pioneering research opens up promising expectations in the design of personalized MSNs-based nanotherapies to treat chronic bone infection.Item Advances in mesoporous silica-based nanocarriers for co-delivery and combination therapy against cancer(Expert Opinion on Drug Delivery, 2016) Castillo Romero, Rafael; Colilla Nieto, Montserrat; Vallet Regí, María Dulce NombreIntroduction: Nanocarriers have emerged as a powerful alternative for cancer therapy.Indeed, they are promising candidates to tackle the acquired resistance of surviving cells against antiproliferative drugs – the so-called multidrug resistance (MDR) phenomenon – which has arisen as one of the major clinical issues of chemotherapy. Among nanocarriers, this review focuses on the recent approaches based on tailored mesoporous silica nanoparticles (MSNs) that could overcome this problem. Areas covered: Herein we summarize the current efforts developed to provide MSNbased nanosystems of enhanced dual therapeutic action against diseased cells. This can be accomplished by three main approaches: i) increasing nanosystems’ killing capability towards particular cells by enhancing both recognition and specificity; ii)increasing the apoptotic effect throughout co-delivery of several drugs; or iii)combining drug delivery with apoptosis induced by physical methods. Expert Opinion: The development of multifunctional nanosystems able to exert the optimal therapeutic action through the minimal administration constitutes a major challenge in nanomedicine. Recent developments in advanced MSN-based platforms for drug delivery represent promising avenues in the management of MDR associated with cancer therapy. All strategies discussed in this manuscript demonstrate improvements against difficult-to-treat tumors.Item Advances in mesoporous silica nanoparticles for targeted stimuli-responsive drug delivery(Expert Opinion on Drug Delivery, 2015) Baeza, Alejandro; Colilla Nieto, Montserrat; Varas Muriel, María José; Vallet Regí, María Dulce NombreMesoporous silica nanoparticles (MSNPs)are one of the most promising inorganic drug delivery systems(DDSs). The design and development of tumour-targeted MSNPs with stimuli-responsive drug release capability aim at enhancing the efficiency and minimising the side effects of antitumour drugs for cancer therapy.