Person:
Colilla Nieto, Montserrat

First Name

Montserrat

Last Name

Colilla Nieto

URI

https://hdl.handle.net/20.500.14352/77764

Affiliation

Universidad Complutense de Madrid

Faculty / Institute

Farmacia

Department

Química en Ciencias Farmacéuticas

Area

Química Inorgánica

Identifiers

Full item page

Search Results

Now showing 1 - 10 of 21

Preventing bacterial adhesion on scaffolds for bone tissue engineering
(International Journal of Bioprinting, 2016) Sánchez Salcedo, Sandra; Colilla Nieto, Montserrat; Izquierdo Barba, Isabel; Vallet Regí, María Dulce Nombre
Bone implant infection constitutes a major sanitary concern which is associated to high morbidity and health costs. This manuscript focused on overviewing the main research efforts committed up to date to develop innovative alternatives to conventional treatments, such as those with antibiotics. These strategies mainly rely on chemical modifi-cations of the surface of biomaterials, such as providing it of zwitterionic nature, and tailoring the nanostructure surface of metal implants. These surface modifications have successfully allowed inhibition of bacterial adhesion, which is the first step to implant infection, and preventing long-term biofilm formation compared to pristine materials. These strate-gies could be easily applied to provide three-dimensional (3D) scaffolds based on bioceramics and metals, of which its manufacture using rapid prototyping techniques was reviewed. This opens the gates for the design and development of advanced 3D scaffolds for bone tissue engineering to prevent bone implant infections. Keywords: Antibacterial adhesion, biofilm formation, zwitterionic surfaces, nanostructured surfaces, rapid prototyping 3D scaffolds, bone tissue engineering.
Project number: 52
Aprendizaje autónomo del laboratorio de Química Bioinorgánica y Biomateriales y técnicas de caracterización utilizadas a través del uso de herramientas e-learning. Potenciación de la comprensión de alumnos con diversidad, dificultades auditivas o idioma
(2022) Sanchez Salcedo, Sandra; Pablos, Jesús Luis; Peña Lopez, Juan; Arcos Navarrete, Daniel; Doadrio Villarejo, Antonio Luis; Garcia Fontecha, Ana; Colilla Nieto, Montserrat; Gonzalez Ortiz, Blanca; Cabañas Criado, María Victoria; Cicuéndez Maroto, Mónica; Salinas Sanchez, Antonio Jesús; Lozano Borregon, Daniel; Manzano Garcia, Miguel; Izquierdo Barba, Isabel; Jimenez Holguin, Javier; Estevez Amado, Manuel; Doadrio Villarejo, Juan Carlos; Moreno Pérez, José Manuel
El proyecto tiene como objetivo fundamental la elaboración de material audiovisual para la mejora de las prácticas de la asignatura de Química Bioinorgánica y Biomateriales de cuarto curso del grado en Farmacia para su satisfactorio desarrollo tanto en un escenario totalmente on-line como presencial. Para el buen desarrollo de las prácticas en el laboratorio es necesario que el alumno disponga de una información adecuada que le permita conocer los distintos aspectos del proceso experimental a realizar a través de la elaboración de una página web disponible en el campus virtual. Para ello, la práctica habitual es utilizar un manual de prácticas de laboratorio en el que se aporta información sobre conceptos básicos, material a utilizar, procedimiento experimental, seguridad, etc… de cada práctica. Hay tres aspectos de gran importancia desde el punto de vista formativo: 1) afianzar bien los conceptos básicos en los que se apoya el trabajo experimental posterior, 2) el manejo de los software que permitan el tratamiento de los resultados e interpretación de los datos utilizando herramientas e-learning y 3) facilitar la comprensión de los dos objetivos anteriores a personas con discapacidades auditivas y/o idioma incluyendo subtítulos en español e inglés en los videos elaborados. En dicha elaboración se tendrá muy en cuenta, la inclusión de los alumnos con diversidad presentando dicha información de manera visual, estructurada, secuencial y en pasos ordenados. Estas herramientas de aprendizaje estarán dirigidas inicialmente a los alumnos de cursos superiores del Grado en Farmacia, Química, Ingeniería de Materiales y Máster Universitario en Biomateriales por lo que para comprobar su impacto y eficacia seleccionaemos a estudiantes de los departamentos implicados antes de ponerlos a disposición de los alumnos. Este proyecto se basa en tres Competencias transversales instrumentales del Espacio Europeo de Educación Superior (EEES): Comunicación oral y escrita en la/s lengua/s materna/s, Comunicación en lengua extranjera, y Utilización de las Tecnologías de la información y la Comunicación (TIC) en el ámbito de estudio y contexto profesional. Estas competencias son clave para el desarrollo académico y profesional de los alumnos/as, especialmente para alumnos/as con algún tipo de discapacidad. Creemos que, mediante este proyecto, se realiza un esfuerzo para mejorar la inclusión de estos alumnos/as tanto en un contexto online como presencial en un laboratorio de Química. Por las razones anteriormente expuestas, un grupo de profesores de los Departamentos de Química en Ciencias Farmacéuticas y el de Bioquímica y Biología Molecular se propone elaborar un material didáctico que permita el autoaprendizaje del alumno en la parte experimental de la asignatura optativa de Química Bioinorgánica y Biomateriales del Grado en Farmacia, si bien, este material también será de utilidad para complementar otras asignaturas presentes en los Grados de Biología o de Ingeniería de Materiales e, incluso en el Máster en Biomateriales de la UCM.
Amine-Functionalized Mesoporous Silica Nanoparticles: A New Nanoantibiotic for Bone Infection Treatment
(Biomedical Glasses, 2018) Pedraza, Daniel; Díez, Jaime; Izquierdo Barba, Isabel; Colilla Nieto, Montserrat; Vallet Regí, María Dulce Nombre
This manuscript reports an effective new alternative for the management of bone infection by the 5 development of an antibiotic nanocarrier able to penetrate bacterial biofilm, thus enhancing antimicrobial effectiveness. This nanosystem, also denoted as “nanoantibiotic”, consists in mesoporous silica nanoparticles (MSNs) loaded with an antimicrobial agent (levofloxacin, LEVO) 10 and externally functionalized with N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (DAMO) as targeting agent. This amine functionalization provides MSNs of positive charges, which improves the affinity towards the negatively charged bacteria wall and biofilm. Physical and 15 chemical properties of the nanoantibiotic were studied using different characterization techniques, including Xray diffraction (XRD), transmission electron microscopy (TEM), N2 adsorption porosimetry, elemental chemical analysis, dynamic light scattering (DLS), zeta (� )-potential 20 and solid-state nuclear magnetic resonance (NMR). “In vial” LEVO release profiles and the in vitro antimicrobial effectiveness of the different released doses were investigated. The efficacy of the nanoantibiotic against a S. aureus biofilm was also determined, showing the practically total 25 destruction of the biofilmdue to the high penetration ability of the developed nanosystem. These findings open up promising expectations in the field of bone infection treatment.
Concanavalin A-targeted mesoporous silica nanoparticles for infection treatment.
(Acta Biomaterialia, 2019) Martínez Carmona, Marina; Izquierdo Barba, Isabel; Colilla Nieto, Montserrat; Vallet Regí, María Dulce Nombre
The ability of bacteria to form biofilms hinders any conventional treatment for chronic infections and has serious socio-economic implications. For this purpose, a nanocarrier capable of overcoming the barrier of the mucopolysaccharide matrix of the biofilm and releasing its loadedantibiotic within this matrix would be desirable. Herein, we developed a new nanosystem based on levofloxacin (LEVO)-loaded mesoporous silica nanoparticles (MSNs) decorated with the lectin concanavalin A (ConA). The presence of ConA promotes the internalization of this nanosystem into the biofilm matrix, which increases the antimicrobial efficacy of the antibiotic hosted within the mesopores. This nanodevice is envisioned as a promising alternative to conventional treatments for infection by improving the antimicrobial efficacy and reducing side effects.
Impact of the antibiotic-cargo from MSNs on gram-positive and gram-negative bacterial biofilms.
(Microporous and Mesoporous Materials, 2020) Aguilar Colomer, Anna; Colilla Nieto, Montserrat; Izquierdo Barba, Isabel; Jiménez Jiménez, Carla; Mahillo, Ignacio; Esteban, Jaime; Vallet Regí, María Dulce Nombre
Mesoporous silica nanoparticles (MSNs) are promising drug nanocarriers for infection treatment. Many investigations have focused on evaluating the capacity of MSNs to encapsulate antibiotics and release them in a controlled fashion. However, little attention has been paid to determine the antibiotic doses released from these nanosystems that are effective against biofilm during the entire release time. Herein, we report a systematic and quantitative study of the direct effect of the antibiotic-cargo released from MSNs on Gram-positive and Gram-negative bacterial biofilms. Levofloxacin (LVX), gentamicin (GM) and rifampin (RIF) were separately loaded into pure-silica and amino-modified MSNs. This accounts for the versatility of these nanosystems since they were able to load and release different antibiotic molecules of diverse chemical nature. Biological activity curves of the released antibiotic were determined for both bacterial strains, which allowed to calculate the active doses that are effective against bacterial biofilms. Furthermore, in vitro biocompatibility assays on osteoblast-like cells were carried out at different periods of times. Albeit a slight decrease in cell viability was observed at the very initial stage, due to the initial burst antibiotic release, the biocompatibility of these nanosystems is evidenced since a recovery of cell viability was achieved after 72 h of assay. Biological activity curves for GM released from MSNs exhibited sustained patterns and antibiotic doses in the 2-6 µg/mL range up to 100 h, which were not enough to eradicate biofilm. In the case of LVX and RIF first-order kinetics featuring an initial burst effect followed by a sustained release above the MIC up to 96 h were observed. Such doses reduced by 99.9% bacterial biofilm and remained active up to 72 h with no emergence of bacterial resistance. This pioneering research opens up promising expectations in the design of personalized MSNs-based nanotherapies to treat chronic bone infection.
Project number: 138
Elaboración de una tabla periódica interactiva de los elementos químicos y de sus propiedades ordenadas con acceso por internet
(2017) Doadrio Villarejo, Antonio Luis; Izquierdo Barba, Isabel; Colilla Nieto, Montserrat; Martínez Alonso, África; Sánchez Salcedo, Sandra; García Fontecha, Ana; Cabañas Criado, María Victoria; Peña López, Juan; Román Zaragoza, Jesús; Salinas Sánchez, Antonio J.; Doadrio Villarejo, Juan Carlos; Gutierrez Rios, Maria Teresa; Arcos Navarrete, Daniel; Moreno Pérez, José Manuel; Vallet Regí, María Dulce Nombre; Sánchez Montero, José
Se ha construido una base de datos con las propiedades de los elementos químicos, isótopos naturales y artificiales de los elementos y el tiempo de semivida de los radiactivos, así como, la serie de la tabla periódica a la que pertenecen, carácter metálico/no metálico/metaloide, configuración electrónica y número atómico, etimología, descubridor y año de descubrimiento de cada elemento químico y de alguna/s de sus características o curiosidades más significativas.
Zwitterionic ceramics for biomedical applications
(Acta Biomaterialia, 2016) Izquierdo Barba, Isabel; Colilla Nieto, Montserrat; Vallet Regí, María Dulce Nombre
Bioceramics for bone tissue regeneration, local drug delivery and nanomedicine, are receiving growing attention by the biomaterials scientific community. The design of bioceramics with improved surface properties able to overcome clinical issues is a great scientific challenge. Zwitterionization of surfaces has arisen as a powerful alternative in the design of biocompatible bioceramics capable to inhibit bacterial and non-specific protein adsorption, which opens up new insights into the biomedical applications of these materials. This manuscript reviews the different approaches reported up to date for the synthesis and characterization of zwitterionic bioceramics with potential clinical applications. Statement of Significance Zwitterionic bioceramics are receiving growing attention by the biomaterials scientific community due to their great potential in bone tissue regeneration, local drug delivery and nanomedicines. Herein, the different strategies developed so far to synthesize and characterize zwitterionic bioceramics with potential clinical applications are summarized. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Magnetic colloidal nanoformulations to remotely trigger mechanotransduction for osteogenic differentiation
(Journal of Colloid And Interface Science, 2024) Gutiérrez Estévez, Manuel; Cicuéndez Maroto, Mónica; Colilla Nieto, Montserrat; Vallet Regí, María Dulce Nombre; González Ortiz, Blanca; Izquierdo Barba, Isabel
Nowadays, diseases associated with an ageing population, such as osteoporosis, require the development of new biomedical approaches to bone regeneration. In this regard, mechanotransduction has emerged as a discipline within the field of bone tissue engineering. Herein, we have tested the efficacy of superparamagnetic iron oxide nanoparticles (SPIONs), obtained by the thermal decomposition method, with an average size of 13 nm, when exposed to the application of an external magnetic field for mechanotransduction in human bone marrowderived mesenchymal stem cells (hBM-MSCs). The SPIONs were functionalized with an Arg-Gly-Asp (RGD) peptide as ligand to target integrin receptors on cell membrane and used in colloidal state. Then, a comprehensive and comparative bioanalytical characterization of non-targeted versus targeted SPIONs was performed in terms of biocompatibility, cell uptake pathways and mechanotransduction effect, demonstrating the osteogenic
The role of zwitterionic materials in the fight against proteins and bacteria
(MEDICINES, 2018) Colilla Nieto, Montserrat; Izquierdo Barba, Isabel; Vallet Regí, María Dulce Nombre
Zwitterionization of biomaterials has been heightened to a potent tool to develop biocompatible materials able to inhibit bacterial and non-specific proteins adhesion. This constitutes a major progress in the biomedical field. This manuscript overviews the main functionalization strategies that have been reported up to date to design and develop these advanced biomaterials. On this regard, the recent research efforts dedicated to provide their surface of zwitterionic nature are summarized by classifying biomaterials in two main groups. First, we centre on biomaterials in clinical use, concretely bioceramics and metallic implants. Finally, we revise emerging nanostructured biomaterials, which are receiving growing attention due to their multifunctionality and versatility mainly in the local drug delivery and bone tissue regeneration scenarios.
Achievements in Mesoporous Bioactive Glasses for Biomedical Applications
(Pharmaceutics, 2022) Vallet Regí, María Dulce Nombre; Colilla Nieto, Montserrat; Izquierdo Barba, Isabel; Vitale Brovarone, Chiara; Fiorilli, Sonia
Nowadays, mesoporous bioactive glasses (MBGs) are envisaged as promising candidates in the field of bioceramics for bone tissue regeneration. This is ascribed to their singular chemical composition, structural and textural properties and easy-to-functionalize surface, giving rise to accelerated bioactive responses and capacity for local drug delivery. Since their discovery at the beginning of the 21st century, pioneering research efforts focused on the design and fabrication of MBGs with optimal compositional, textural and structural properties to elicit superior bioactive behavior. The current trends conceive MBGs as multitherapy systems for the treatment of bonerelated pathologies, emphasizing the need of fine-tuning surface functionalization. Herein, we focus on the recent developments in MBGs for biomedical applications. First, the role of MBGs in the design and fabrication of three-dimensional scaffolds that fulfil the highly demanding requirements for bone tissue engineering is outlined. The different approaches for developing multifunctional MBGs are overviewed, including the incorporation of therapeutic ions in the glass composition and the surface functionalization with zwitterionic moieties to prevent bacterial adhesion. The bourgeoning scientific literature on MBGs as local delivery systems of diverse therapeutic cargoes (osteogenic/antiosteoporotic, angiogenic, antibacterial, anti-inflammatory and antitumor agents) is addressed. Finally, the current challenges and future directions for the clinical translation of MBGs are discussed.